Responses of biological characteristics and detoxification enzymes in the fall armyworm to methoxyfenozide stress

Journal of Economic Entomology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 20, 2025

Abstract Methoxyfenozide is an insecticide with a unique mode of action on the insect ecdysone receptor and has been registered for control pests all over world. In present work, Spodoptera frugiperda was exposed to sublethal lethal concentrations methoxyfenozide determine its impact specific biological traits, metabolic enzyme activity, expression detoxification enzymes. The result showed that 72-h posttreatment LC50 LC70 significantly reduced fecundity (eggs/female) F0 generation compared those group. However, duration prepupal period increased. exposure LC10, LC30, LC50, extended developmental larvae in F1 individuals. decreased, population life table parameters were also affected. activity carboxylesterases little significant change, whereas glutathione S-transferases (GSTs) cytochrome P450 monooxygenases (P450s) altered after methoxyfenozide. total, 24-, 48-, 96-h could cause upregulation genes such as CYP6AE44, CYP6B39, CYP9A26, CYP9A58, CYP9A59, CYP9A60, well GST including GSTe3, GSTe9, GSTe10, GSTe15, GSTo2, GSTs1, GSTs5, GSTm2, GSTm3. These findings be instrumental elucidating molecular mechanisms underlying effects S. frugiperda.

Language: Английский

Transcriptome Analysis of Insecticide Resistance Mechanisms in Field Populations of the Bean Flower Thrips, Megalurothrips Usitatus (Bagnall)

Published: Jan. 1, 2025

Language: Английский

Functional Analysis of Insecticide Inhibition and Metabolism of Six Glutathione S-Transferases in the Rice Stem Borer, Chilo suppressalis

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(22), P. 12489 - 12497

Published: May 22, 2024

The glutathione S-transferases (GSTs) are important detoxifying enzymes in insects. Our previous studies found that the susceptibility of Chilo suppressalis to abamectin was significantly increased when CsGST activity inhibited by (GSH) depletory. In this study, potential detoxification mechanisms CsGSTs were explored. Six C. expressed vitro. Enzymatic kinetic parameters including Km and Vmax recombinant determined, results showed all six catalytically active displaying transferase activity. Insecticide inhibitions revealed a low concentration could effectively inhibit activities CsGSTd1, CsGSTe4, CsGSTo2, CsGSTs3, CsGSTu1. However, vitro metabolism assay not metabolize directly. Additionally, post-treatment with abamectin. Comprehensive analysis present our demonstrated play an role catalyzing conjugation GSH suppressalis, high binding affinities CsGSTu1 might also suggest involvement via noncatalytic passive sequestration instead direct metabolism. These helpful better understand GSTs

Language: Английский