Discovery and characterization of cyclotides, plant-based peptides from <i>Viola dalatensis</i> Gadnep DOI
Son H. Pham, Tran Thi Thanh Tam,

Pham Nguyen Dong Yen

et al.

Vietnam Journal of Biotechnology, Journal Year: 2024, Volume and Issue: 22(3), P. 450 - 469

Published: Sept. 30, 2024

Cyclotides demonstrate remarkable stability due to their unique characteristic - the cyclic cystine knot motif. exhibit a wide range of biological activities. This study aims explore presence cyclotides in Viola dalatensis Gadnep, plant indigenous Vietnam, through utilization LC-MS and LC-MS/MS techniques. We conducted comprehensive analysis three extraction methods: 50% acetonitrile with 1% formic acid, 70% ethanol, methanol. The initial method is extremely efficient for cyclotide when utilizing analysis. An ammonium sulfate salt concentration 30% used enhance content optimize RP-HPLC purification procedure. precipitates notable advantage terms antibacterial properties compared extracts, particularly decreased by factor four comparison extracts. combination demonstrated potent antimicrobial activity against Bacillus subtilis Pseudomonas aeruginosa. impact was most noticeable mixture ten times lower than precipitates. inhibition zones these bacteria measured 17.17 ± 2.24 mm 20.23 0.84 mm, respectively. identification primary structure nine successfully achieved.

Language: Английский

Advances in cyclotide research: bioactivity to cyclotide-based therapeutics DOI

Ankita Grover,

Sawraj Singh,

Sonal Sindhu

et al.

Molecular Diversity, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 25, 2025

Language: Английский

Citations

0
Exploring the fumigant potential of Artemisia subg. Seriphidium essential oils and their dominant constituents against the red imported fire ants Solenopsis invicta DOI Creative Commons
Syed Arif Hussain Rizvi, Ying Li,

Rana Muhammad Kaleem Ullah

et al.

Industrial Crops and Products, Journal Year: 2025, Volume and Issue: 226, P. 120603 - 120603

Published: Feb. 4, 2025

Language: Английский

Citations

0
Transgenic Innovation: Harnessing Cyclotides as Next Generation Pesticides DOI Creative Commons

Sathira Deegala,

Hiruni C. Rathnapala,

Sanjeevan Rajendran

et al.

ACS Omega, Journal Year: 2025, Volume and Issue: 10(7), P. 6323 - 6336

Published: Feb. 17, 2025

Cyclotides are unique cyclic mini proteins derived from plants which recognized for the distinctive cystine knot (CCK) structure and cyclized backbone. To date, more than 760 sequences of cyclotides have been identified across five major families, making them largest known group peptides. These peptides garnered significant attention due to their remarkable structural stability diverse bioactivities, including potent insecticidal properties, offer a promising alternative conventional pesticides that often associated with environmental toxicity resistance development in pests. Advances transgenic technology opened new avenues sustainable targeted deployment pest management. By incorporating cyclotide genes into crops, can gain enhanced self-defense mechanisms against insect pests, reducing reliance on chemical mitigating ecological impact. This review explores molecular features essential cyclotides' activity, latest breakthroughs strategies expression potential challenges future prospects this innovative approach. highlighting synergy between natural bioactive compounds genetic engineering, work underscores as next-generation, eco-friendly biopesticides address global agricultural challenges.

Language: Английский

Citations

0
Cyclotides from Violaceae: Isolation, characterization, and potential applications DOI

Uyen N. P. Nguyen,

Vũ Đình Hoàng, Phạm Thị Năm

et al.

Vietnam Journal of Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 17, 2025

Abstract Cyclotides are cyclic polypeptides consisting of 28 to 37 residues, distinguished by six cysteine residues organized in a specific arrangement three disulfide linkages. This distinctive structure provides remarkable structural stability and resilience biological deterioration, thermal stress, chemical denaturation. plants function as natural defense mechanism against pests. exhibit various actions that could be applied agriculture pharmaceuticals. Numerous cyclotides have been discovered plant families including Violaceae, Rubiaceae, Solanaceae, Cucurbitaceae, Poaceae, Fabaceae. They predominantly located succeeded with limited occurrences other families. The Violaceae family, 98% species categorized within the genera Viola , Rinorea Hybanthus exhibits notable diversity Vietnam. Although genus is prevalent, also has considerable diversity. Despite abundance Vietnam, there significant deficiency studies about extracting chemicals from this family. These underscore relevance cyclotide research highlighting their extensive potential across multiple domains emphasizing necessity advancing such research, especially

Language: Английский

Citations

0
Enzymes, Proteins, and Peptides as Promising Biosolutions for Crop Production DOI

Baptiste Kergaravat,

Laure Plener,

Magali Castan

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: May 1, 2025

The development of environmentally friendly alternatives to plant protection products is essential for sustainable agriculture. In this context, natural compounds, such as enzymes, proteins, and peptides, represent a promising reservoir bioactive molecules. Although little represented on the market, their potential fertilizers, biostimulants, biopesticides highlighted by numerous reports patents, which present review aims bring together. While fertilization biostimulation are primarily aimed at improving yield health, can be used target wide range microbial pathogens pests. nature effects proteinaceous molecules discussed in light restrictive regulations.

Language: Английский

Citations

0
Gene-guided identifications of a structure-chimeric cyclotide viphi I from Viola philippica: potential functions against cadmium and nematodes DOI
Xiaojie Zhang, Ruohong Chen, Haoyue Shu

et al.

Plant Science, Journal Year: 2024, Volume and Issue: 347, P. 112185 - 112185

Published: July 8, 2024

Language: Английский

Citations

1
Potential and Limitation of Peptides from Native Plants of Uttarakhand DOI
Neha Kamboj, Rahul Kumar, Navin Kumar

et al.

International Journal of Peptide Research and Therapeutics, Journal Year: 2024, Volume and Issue: 30(5)

Published: Sept. 3, 2024

Language: Английский

Citations

1
Characterization of cyclotides Mra30 and cycloviolacin O17 derived from Viola dalatensis Gadnep DOI
Tien T. Dang, Tam T. T. Tran, Son H. Pham

et al.

Archives of Microbiology, Journal Year: 2024, Volume and Issue: 206(10)

Published: Sept. 9, 2024

Language: Английский

Citations

1
New Insights into Involvement of Low Molecular Weight Proteins in Complex Defense Mechanisms in Higher Plants DOI Open Access
Magdalena Ruszczyńska, Hubert Sytykiewicz

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(15), P. 8531 - 8531

Published: Aug. 5, 2024

Dynamic climate changes pose a significant challenge for plants to cope with numerous abiotic and biotic stressors of increasing intensity. Plants have evolved variety biochemical molecular defense mechanisms involved in overcoming stressful conditions. Under environmental stress, generate elevated amounts reactive oxygen species (ROS) and, subsequently, modulate the activity antioxidative enzymes. In addition, an increase biosynthesis important plant compounds such as anthocyanins, lignin, isoflavonoids, well wide range low weight stress-related proteins (e.g., dehydrins, cyclotides, heat shock pathogenesis-related proteins), was evidenced. The induced expression these improves survival rate under unfavorable stimuli enhances their adaptation sequentially interacting stressors. Importantly, may also potential use medical applications agriculture biopesticides). Therefore, it is gain more thorough understanding complex biological functions proteins. It will help devise new cultivation strategies, including development genotypes characterized by better adaptations adverse review presents latest research findings on selected

Language: Английский

Citations

0
Discovery and characterization of cyclotides, plant-based peptides from <i>Viola dalatensis</i> Gadnep DOI
Son H. Pham, Tran Thi Thanh Tam,

Pham Nguyen Dong Yen

et al.

Vietnam Journal of Biotechnology, Journal Year: 2024, Volume and Issue: 22(3), P. 450 - 469

Published: Sept. 30, 2024

Cyclotides demonstrate remarkable stability due to their unique characteristic - the cyclic cystine knot motif. exhibit a wide range of biological activities. This study aims explore presence cyclotides in Viola dalatensis Gadnep, plant indigenous Vietnam, through utilization LC-MS and LC-MS/MS techniques. We conducted comprehensive analysis three extraction methods: 50% acetonitrile with 1% formic acid, 70% ethanol, methanol. The initial method is extremely efficient for cyclotide when utilizing analysis. An ammonium sulfate salt concentration 30% used enhance content optimize RP-HPLC purification procedure. precipitates notable advantage terms antibacterial properties compared extracts, particularly decreased by factor four comparison extracts. combination demonstrated potent antimicrobial activity against Bacillus subtilis Pseudomonas aeruginosa. impact was most noticeable mixture ten times lower than precipitates. inhibition zones these bacteria measured 17.17 ± 2.24 mm 20.23 0.84 mm, respectively. identification primary structure nine successfully achieved.

Language: Английский

Citations

0