Regulatory Mechanisms in Biosystems,
Journal Year:
2024,
Volume and Issue:
15(4), P. 868 - 874
Published: Oct. 26, 2024
Lung
cancer
is
the
leading
cause
of
death
from
in
Ukraine
and
worldwide.
The
impact
on
tumor
microenvironment
most
promising
direction
for
lung
therapy.
Tumor-associated
macrophages
type
M2
have
powerful
immunosuppressive
properties.
A
new
potential
way
influencing
signaling
protein
activator
transcription
6
(STAT6).
aim
our
study
was
to
evaluate
role
STAT6
formation
prognosis
patients
with
radically
treated
non-small
cell
(NSCLC).
We
performed
an
immunohistochemical
examination
tissue
42
NSCLC
antibodies
CD8+,
forkhead
box
P3
(FOXP3+),
CD163+,
STAT6.
survival
assessed
by
Cox
regression
analysis.
median
follow-up
period
studied
cohort
57.9
±
4.2
months.
50.0%
high
expression
established
that
correlates
histology
gender
patients.
High
significantly
more
determined
squamous
carcinomas
than
adenocarcinomas.
In
carcinomas,
metastasis
regional
lymph
nodes
associated
immune
exclusion
phenotype
mediated
a
infiltration
stroma
macrophages.
An
inflammatory
low
FOXP3+,
typical
adenocarcinoma.
Low
are
common
women;
men.
Females
CD8+
clusters,
CD163+
stroma,
better
overall
survival.
Conclusions:
overexpression
has
negative
recurrence-free
To
obtain
accurate
results,
it
necessary
conduct
includes
larger
patients,
particular,
female
Chemical Biology & Drug Design,
Journal Year:
2025,
Volume and Issue:
105(1)
Published: Jan. 1, 2025
ABSTRACT
Bazhen
Decoction
(Eight
Treasures
Decoction)
has
demonstrated
efficacy
in
the
treatment
of
colorectal
cancer
(CRC),
yet
active
ingredients
it
and
mechanisms
underlying
their
anti‐cancer
properties
are
not
well
understood.
Through
network
pharmacology,
effective
components
against
CRC
corresponding
key
genes
were
delineated.
Molecular
docking
was
executed
to
identify
component
targeting
gene
CXCL8,
which
led
discovery
Quercetin.
The
cellular
thermal
shift
assay
method
then
used
verify
binding
interaction.
cells
treated
with
incremental
concentrations
Quercetin,
cell
viability
evaluated
by
Cell
Counting
Kit‐8
calculate
IC
50
,
apoptosis
rates
determined
flow
cytometry.
Expression
apoptosis‐related
proteins
Bcl‐2
Cleaved
caspase‐3
measured
using
western
blot.
impact
Quercetin
on
macrophage
polarization
studied
co‐culturing
macrophages,
assessing
M1
M2
distribution
via
cytometry,
quantifying
cytokine
levels
(IL‐6,
IL‐10,
IL‐12,
CXCL8)
enzyme‐linked
immunosorbent
(ELISA).
ingredient
from
exhibited
a
targeted
affinity
enabled
inhibit
proliferation
induce
apoptosis.
overexpression
CXCL8
associated
promotion
malignancy,
presence
could
lessen
cells.
Moreover,
leads
diminished
abundance
macrophages
an
increase
cytokines
IL‐6
while
reducing
IL‐10
indicates
that
inhibitory
effect
polarization.
is
known
for
anti‐CRC
effects,
targets
inhibits
impede
malignant
behaviors
macrophages.
Thus,
may
be
utilized
as
immunomodulatory
agent
treatment.
Discover Oncology,
Journal Year:
2025,
Volume and Issue:
16(1)
Published: March 25, 2025
M2-like
tumor-associated
macrophages
(M2-like
TAMs)
function
crucially
in
the
tumor
microenvironment
(TME)
and
cancer
development.
This
study
developed
a
prognostic
signature
based
on
TAM-related
genes
for
breast
(BRCA)
applying
transcriptome
scRNA-seq
analysis.
TCGA-BRCA,
GSE20685,
GSE176078
datasets
were
downloaded
from
UCSC
xena
GEO
databases.
AUCell
score
of
immune-related
(IRGs)
was
calculated
using
R
package.
Genes
related
to
TAMs
screened
by
WGCNA.
Prognostic
further
identified
univariate
Cox
LASSO
regression
analyses
form
RiskScore
model,
which
validated
external
dataset.
Furthermore,
nomogram
established
integrating
clinical
characteristics,
correlation
analysis
between
TME
or
chemotherapeutic
drugs
conducted.
Finally,
mRNA
expression
levels
key
verified
quantitative
real
time
polymerase
chain
reaction
(qRT-PCR).
As
exhibited
highest
IRGs
single-cell
transcriptomic
atlas
BRCA,
cells
classified
into
Macrophages
C1
C2
subtypes,
with
subtype
showing
high
M2
macrophage
marker
genes.
ARHGAP26,
RILP,
KLRB1,
CSTA,
KLHDC7B,
PSMB8,
KYNU,
RNASE1,
LONRF3,
TRPM2
as
total
903
establish
robust
model.
strong
predictive
performance
constructed
combining
stage,
Age,
RiskScore,
we
found
that
most
immune
showed
negative
RiskScore.
Multiple
closely
associated
notably,
Ribociclib_1632
had
higher
half-maximal
inhibitory
concentration
(IC50)
value
high-risk
group.
qRT-PCR
demonstrated
10
significantly
different
control
BRCA
cell
lines.
We
providing
potential
therapeutic
targets
treatment
cancer.
Biomedicine & Pharmacotherapy,
Journal Year:
2024,
Volume and Issue:
172, P. 116269 - 116269
Published: Feb. 16, 2024
AGS-30,
a
new
andrographolide
derivative,
showed
significant
anticancer
and
anti-angiogenic
characteristics.
However,
its
role
in
controlling
macrophage
polarization
tumor
immune
response
is
unknown.
Thus,
the
main
goals
of
this
study
are
to
investigate
how
AGS-30
regulates
it
suppresses
breast
cancer
metastasis.
inhibited
IL-4
IL-13-induced
RAW
264.7
THP-1
macrophages
into
M2-like
phenotype.
did
not
affect
LPS
IFN-γ-induced
M1-like
macrophages.
reduced
mRNA
expressions
CD206,
Arg-1,
Fizz-1,
Ym-1,
VEGF,
IL-10,
MMP2,
MMP9
concentration-dependent
manner.
In
contrast,
treatment
at
5
μM
polarization.
The
conditioned
medium
from
increased
4T1
cell
migration
invasion,
whereas
these
effects.
xenograft
mice,
volume
weight
were
without
affecting
body
after
receiving
AGS-30.
also
lung
liver
metastasis,
with
STAT6,
CD31,
Ki67
protein
expressions.
Moreover,
tumors
had
considerably
fewer
Arg-1
expression,
but
proportion
iNOS
expression
treatment.
Same
results
found
tail
vein
metastasis
model.
conclusion,
shows
that
inhibits
growth
probably
through
inhibiting
Our
findings
suggest
may
be
potential
immunotherapeutic
alternative
for
metastatic
cancer.
