Fraxin Alleviates Atherosclerosis by Inhibiting Oxidative Stress and Inflammatory Responses via the TLR4/PI3K/Akt Pathway DOI Creative Commons
Yaru Wang,

Bailing Wei,

Mingyang Leng

et al.

Current Issues in Molecular Biology, Journal Year: 2025, Volume and Issue: 47(5), P. 308 - 308

Published: April 27, 2025

Fraxin is a bioactive compound derived from Cortex Fraxini. It known for its diverse biological activities and numerous benefits, including anti-inflammatory, antioxidant, analgesic, antimicrobial, antiviral, immunomodulatory effects. Despite growing interest in natural compounds cardiovascular diseases Fraxin’s atheroprotective properties molecular targets have not yet been fully elucidated. To address this gap, our research employed an integrated approach combining network pharmacology, docking simulations, vitro validation to systematically unravel therapeutic mechanisms against atherosclerosis (AS). The results showed that 84 potential AS were predicted through public databases, the key target TLR4 was identified by protein–protein interaction analysis. GO enrichment KEGG pathway analysis revealed these significantly enriched PI3K-Akt oxidative stress responses pathways. Subsequently conducted studies validated modulates TLR4/PI3K/Akt signaling suppress reactive oxygen species generation downregulate pro-inflammatory cytokines Il1b, Il6, Tnf thereby slowing atherosclerotic disease advancement. This investigation methodically delineates underlying management, establishing scientific foundation translation into clinical practice.

Language: Английский

Fraxin inhibits ovariectomized-induced bone loss and osteoclastogenesis by suppressing ROS activity DOI
Han Zhou,

Pianpian Chen,

Chengyi Zhao

et al.

International Immunopharmacology, Journal Year: 2025, Volume and Issue: 147, P. 113871 - 113871

Published: Jan. 10, 2025

Language: Английский

Citations

0
Lipid peroxidation inhibition by icaritin and its glycosides as a strategy to combat iron overload-induced osteoporosis in zebrafish DOI
Chang‐Yu Yan,

Xiao‐yuan Gu,

Si Tan

et al.

Food Research International, Journal Year: 2025, Volume and Issue: 203, P. 115900 - 115900

Published: Feb. 1, 2025

Language: Английский

Citations

0
Astaxanthin Prevents Glucocorticoid-Induced Femoral Head Osteonecrosis by Targeting Ferroptosis through the JAK2/STAT3 Signaling Pathway DOI

Yuzhe Lin,

Zi-hao Chen,

Jianfeng Yang

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 4, 2025

Glucocorticoid (GC) is extensively used in clinical practice, and the osteonecrosis of femoral head caused by them a common issue orthopedic surgery, yet underlying mechanisms remain unclear. Astaxanthin (AST), potent natural antioxidant, has an unexplored impact on GC-induced (GIONFH). This study explores effects AST counteracting dexamethasone (Dex)-induced ferroptosis GIONFH. We developed rat model GIONFH using intraperitoneal Dex injections conducted vitro analysis culturing osteoblasts (OBs) with treatment. assessed Dex-treated OBs C11-BODIPY FerroOrange staining, mitochondrial functionality tests, protein expression analyses through Western blot immunofluorescence. The influence bone microarchitecture was micro-CT, hematoxylin eosin immunofluorescence, immunohistochemistry at imaging histological levels. Our findings suggest that exerts inhibitory effect Dex-induced In vitro, treatment increased glutathione decreased malondialdehyde, lipid peroxidation, mitochondrial-reactive oxygen species. Additionally, also enhances phosphorylation STAT3, upregulates peroxidase 4 osteogenic-related proteins, stimulates formation. To delve deeper into mechanism, revealed triggered activation JAK2/STAT3 signaling. Moreover, use siRNA-STAT3 blocked beneficial cultivated Dex. brief, combats activating pathway to inhibit ferroptosis.

Language: Английский

Citations

0
Fraxin Alleviates Atherosclerosis by Inhibiting Oxidative Stress and Inflammatory Responses via the TLR4/PI3K/Akt Pathway DOI Creative Commons
Yaru Wang,

Bailing Wei,

Mingyang Leng

et al.

Current Issues in Molecular Biology, Journal Year: 2025, Volume and Issue: 47(5), P. 308 - 308

Published: April 27, 2025

Fraxin is a bioactive compound derived from Cortex Fraxini. It known for its diverse biological activities and numerous benefits, including anti-inflammatory, antioxidant, analgesic, antimicrobial, antiviral, immunomodulatory effects. Despite growing interest in natural compounds cardiovascular diseases Fraxin’s atheroprotective properties molecular targets have not yet been fully elucidated. To address this gap, our research employed an integrated approach combining network pharmacology, docking simulations, vitro validation to systematically unravel therapeutic mechanisms against atherosclerosis (AS). The results showed that 84 potential AS were predicted through public databases, the key target TLR4 was identified by protein–protein interaction analysis. GO enrichment KEGG pathway analysis revealed these significantly enriched PI3K-Akt oxidative stress responses pathways. Subsequently conducted studies validated modulates TLR4/PI3K/Akt signaling suppress reactive oxygen species generation downregulate pro-inflammatory cytokines Il1b, Il6, Tnf thereby slowing atherosclerotic disease advancement. This investigation methodically delineates underlying management, establishing scientific foundation translation into clinical practice.

Language: Английский

Citations

0