ProAKAP4 as Indicator of Long-Lasting Motility Marker in Post-Thaw Conditions in Stallions DOI Creative Commons
Marta Dordas‐Perpinyà, Iván Yánez-Ortiz, Nicolas Sergeant

et al.

Animals, Journal Year: 2024, Volume and Issue: 14(9), P. 1264 - 1264

Published: April 23, 2024

ProAKAP4, a precursor of AKAP4 (A-kinase anchor protein) found in the flagellum mammalian and non-mammalian spermatozoa, serves as structural protein with established correlations to motility parameters across diverse species. This study aimed determine proAKAP4 level evolution thawed stallion semen over 3 h period, examining its correlation descriptors mitochondrial membrane potential. Utilizing sixteen ejaculates from four French warmblood stallions, this involved maintaining samples at 37 °C for h, conducting enzyme-linked immunosorbent assays (ELISA), computer-assisted sperm analysis (CASA), potential by JC-1 probe flow cytometry 0, 1, post-thawing. The findings indicate significant positive (p ≤ 0.05) between levels total or progressive all time points analyzed. Spermatozoa velocity (VAP, VCL, VSL) spermatozoa lateral head displacement (ALH) display ProAKAP4 0 concentration exhibits no discernible difference batches without cryoprotectant. Notably, consumption remains insignificant within initial hour after thawing but becomes 1 In summary, demonstrates up thawing, albeit showing noticeable decrease starting first post-thawing, indicating result motile activity.

Language: Английский

ProAKAP4 as Indicator of Post-thaw Motility in Stallion DOI Open Access
Marta Dordas‐Perpinyà, Iván Yánez-Ortiz, Nicolas Sergeant

et al.

Published: March 25, 2024

ProAKAP4, a precursor of AKAP4 (A-kinase anchor protein) found in the flagellum mammalian and non-mammalian spermatozoa, serves as structural protein with established correlations to motility parameters across diverse species. This study aimed determine proAKAP4 levels evolution thawed stallion semen over 3-hour period, examining its correlation descriptors mitochondrial membrane potential. Utilizing sixteen ejaculates from four French warmblood stallions, involved maintaining samples at 37°C for 3 hours, conducting enzyme-linked immunosorbent assay (ELISA), computer-assisted sperm analysis (CASA), potential by JC-1 probe flow cytometry 0, 1, hours post-thawing. The findings indicate significant positive (p≤0.05) between total or progressive all time points analyzed. Spermatozoa velocity (VAP, VCL, VSL) spermatozoa lateral head displacement (ALH) display ProAKAP4 0-hour concentration exhibits no discernible difference batches without cryoprotectant. Notably, consumption remains insignificant within initial hour after thawing but becomes 1 In summary, demonstrates up thawing, albeit showing noticeable decrease starting first post-thawing, indicating result motile activity.

Language: Английский

Citations

4
Elevated Lipid Concentrations in Seminal Plasma Can Reduce Sperm Motility in Simmental Bulls DOI Creative Commons
Zhuo Yang, Fang Luo, Chenglei Song

et al.

Animals, Journal Year: 2025, Volume and Issue: 15(2), P. 276 - 276

Published: Jan. 20, 2025

Sperm motility is a key factor influencing male fertility and associated with metabolic lipid profiles across species. The aim of this study was to investigate the relationship between sperm seminal plasma profile in Simmental bulls, identify lipids potentially motility. Semen samples were collected from 26 healthy bulls an average age 4.9 years. quality evaluated using computer-assisted analysis (CASA). Based on motility, divided into two groups: high (HSM > 65%) low (LSM < 65%). Compared LSM group, HSM group exhibited significantly higher viability, straight-line velocity, beat-cross frequency, acrosome integrity, while malformation rate lower (p 0.05). Lipid determined LC-MS/MS, 40 differential identified by multivariate statistical analysis. Among them, 39 upregulated compared group. They primarily triglycerides carnitines, mainly involved four pathways related glycerophospholipid linoleic acid metabolism. Notably, PC (16:0/20:4; 14:0/18:3), LPC (22:4/0:0; 22:6/0:0), PE (14:0/18:1; 18:1/20:3) diagnosed great accuracy (AUC 0.7), which means they may serve as potential biomarkers for

Language: Английский

Citations

0
ProAKAP4 protein marker: Towards a functional approach to male fertility DOI

Sara Carracedo,

Lamia Briand‐Amirat,

Marta Dordas‐Perpinyà

et al.

Animal Reproduction Science, Journal Year: 2022, Volume and Issue: 247, P. 107074 - 107074

Published: Sept. 22, 2022

Language: Английский

Citations

17
ProAKAP4 Concentration Is Related to Sperm Motility and Motile Sperm Subpopulations in Frozen–Thawed Horse Semen DOI Creative Commons
Marta Dordas‐Perpinyà, Iván Yánez-Ortiz, Nicolas Sergeant

et al.

Animals, Journal Year: 2022, Volume and Issue: 12(23), P. 3417 - 3417

Published: Dec. 4, 2022

ProAKAP4 is the precursor of AKAP4 (A-kinase Anchor protein 4), main structural fibrous sheath sperm. The amount proAKAP4 reflects ability spermatozoa to maintain flagellum activity and functionality up site fertilization positively correlated with progressive motility in several mammalian species. aim this study was investigate relationship between concentration horse sperm descriptors spermatic motile subpopulations. For purpose, a total 48 ejaculates from 13 different stallions were analyzed. Spermatic obtained by CASA system, four subpopulations (SP) specific patterns statistically identified. concentrations evaluated ELISA. evaluated. Following hierarchical cluster statistical analysis, divided into two groups according their concentrations, either having low (5.06−35.61 ng/10M spz; n = 23) or high (39.92−82.23 25) (p < 0.001). 0.05) motility, as well parameters velocity. also showed negative correlation subpopulation number 3, which lowest velocity parameters. In conclusion, stallion semen functional kinematic descriptors. Concentrations higher than 37.77 spz very good quality frozen/thawed semen.

Language: Английский

Citations

14
Influence of sperm cryopreservation on sperm motility and proAKAP4 concentration in mice DOI
Auke Boersma,

Jasmin Primus,

Bettina Wagner

et al.

Reproductive Medicine and Biology, Journal Year: 2022, Volume and Issue: 21(1)

Published: Jan. 1, 2022

The protein proAKAP4 is crucial for sperm motility and has been suggested as an indicator of male fertility. We determined the relationship between concentration parameters in mice, investigated effects cryopreservation on these variables.Computer-assisted analysis ELISA were applied to determine fresh frozen-thawed epididymal SWISS, B6D2F1, C57BL/6N, BALB/c mice.ProAKAP4 levels ranged 12 89 ng/ml did not differ samples, or strains. found a negative some parameters. Sperm traits differed strains, negatively affected velocity but direction parameters.ProAKAP4 mouse spermatozoa unaffected by cryopreservation, highlighting robustness this parameter potentially time-independent marker high individual variation supports potential role quality, though we no positive, even relationships Future studies have investigate significance fertility mice.

Language: Английский

Citations

8
Evaluation of a commercial proAKAP4 kit for the assessment of fresh and frozen–thawed feline spermatozoa DOI
Sylwia Prochowska, Maria Eberhardt, Wojciech Niżański

et al.

Reproduction in Domestic Animals, Journal Year: 2024, Volume and Issue: 59(3)

Published: March 1, 2024

Abstract ProAKAP4 is gaining increasing attention as a potential marker of semen quality in many species, but while there commercial kit for assessing proAKAP4 the domestic cat, are no publications about its use. The aim this study was to evaluate – Cat 4MID® Kit (SPQI 4BioDx, Lille, France) assessment feline semen. Semen collected from 54 male cats by urethral catheterization. After basic evaluation (subjective motility, CASA, viability, morphology), levels each sample were assessed using according manufacturer's protocol or with some modifications related incubation time, storage conditions and number spermatozoa used. Finally, Spearman correlation concentration sperm motility parameters calculated. most reliable results (acceptable intraassay coefficient variation) obtained an optimized overnight isolation protein 1 × 10 6 stored at −80°C. For fresh semen, significant correlations between parameters, despite strong viability morphology. A predominant effect other highly variable performance lysis buffer question usefulness frozen–thawed moderate, negative two CASA VAP VSL. As cryopreserved samples, cannot be used freezability cats. More studies needed establish long‐lasting motility.

Language: Английский

Citations

1
Semen characteristics, freezability, and application of motility-based protein markers (proAKAP4) in assessing the suitability of superior Bali bulls (Bos sondaicus) at the Regional AI Center DOI Creative Commons
Kudratullah Kudratullah, Raden Iis Arifiantini,

Enny Yuliani

et al.

Reproduction and Breeding, Journal Year: 2024, Volume and Issue: 4(4), P. 279 - 286

Published: Oct. 15, 2024

Language: Английский

Citations

0
ProAKAP4 as Indicator of Long-Lasting Motility Marker in Post-Thaw Conditions in Stallions DOI Creative Commons
Marta Dordas‐Perpinyà, Iván Yánez-Ortiz, Nicolas Sergeant

et al.

Animals, Journal Year: 2024, Volume and Issue: 14(9), P. 1264 - 1264

Published: April 23, 2024

ProAKAP4, a precursor of AKAP4 (A-kinase anchor protein) found in the flagellum mammalian and non-mammalian spermatozoa, serves as structural protein with established correlations to motility parameters across diverse species. This study aimed determine proAKAP4 level evolution thawed stallion semen over 3 h period, examining its correlation descriptors mitochondrial membrane potential. Utilizing sixteen ejaculates from four French warmblood stallions, this involved maintaining samples at 37 °C for h, conducting enzyme-linked immunosorbent assays (ELISA), computer-assisted sperm analysis (CASA), potential by JC-1 probe flow cytometry 0, 1, post-thawing. The findings indicate significant positive (p ≤ 0.05) between levels total or progressive all time points analyzed. Spermatozoa velocity (VAP, VCL, VSL) spermatozoa lateral head displacement (ALH) display ProAKAP4 0 concentration exhibits no discernible difference batches without cryoprotectant. Notably, consumption remains insignificant within initial hour after thawing but becomes 1 In summary, demonstrates up thawing, albeit showing noticeable decrease starting first post-thawing, indicating result motile activity.

Language: Английский

Citations

0