Herpes simplex virus type 1 origin binding protein UL9 tethers and loops origin- and non-origin-DNA intra- and intermolecularly DOI Creative Commons
Anita F. Meier,

Joško Vučković,

Paul Girvan

et al.

Published: Oct. 30, 2024

Abstract Herpesviruses are ubiquitous human pathogens, which the causative agent of mild to severe symptoms ranging from cold sore nasopharyngeal carcinoma. Even though replication linear dsDNA genome has been studied for decades, we still lack a complete molecular understanding its mechanism. It proposed, but never shown directly, that HSV-1 origin binding protein UL9 binds two closely located sites within oriS sequence, thereby mediating looping, in turn facilitates initiation. Here, used an array single-molecule approaches test this long-standing hypothesis directly. Surprisingly, data show does not loop efficiently. However, demonstrate can form large DNA loops at non-origin sequences very efficiently, as well tether molecules intermolecularly. Contrary bending hypothesis, our findings indicate DNA, rather may play alternative role initiation, such tethering separate facilitate recombination.

Language: Английский

Extraction methods, structural features and bioactivity diversity of polysaccharides from the genus Chrysanthemum: A review DOI
Peiling Yu,

Yue Zhou,

Jia Liu

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 139423 - 139423

Published: Jan. 1, 2025

Language: Английский

Citations

1
Novel Hsp90α inhibitor inhibits HSV-1 infection by suppressing the Akt/β-catenin pathway DOI
Zexu Wang,

Weixiangmin Zou,

Qiongzhen Zeng

et al.

International Journal of Antimicrobial Agents, Journal Year: 2025, Volume and Issue: 65(3), P. 107448 - 107448

Published: Jan. 23, 2025

Language: Английский

Citations

0
HSV-1 virions and related particles: biogenesis and implications in the infection DOI Creative Commons
Maria Kalamvoki

Journal of Virology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 3, 2025

ABSTRACT Virion formation and egress are sophisticated processes that rely on the spatial temporal organization of host cell membranes manipulation machineries involved in protein sorting, membrane bending, fusion, fission. These result infectious virions, defective particles, various vesicle-like structures. In herpes simplex virus 1 (HSV-1) infections, virions capsid-less known as light (L)-particles, formed. HSV-1 infection also stimulates release particles resemble extracellular vesicles (EVs). productively infected cells, most EVs generated through CD63 tetraspanin biogenesis pathway lack viral components. A smaller subset EVs, endosomal sorting complexes required for transport (ESCRT) pathway, contains both factors. Viral mechanisms tightly regulate EV biogenesis, including inhibition autophagy—a process critical increased production CD63+ during infection. Mutant viruses fail to suppress autophagy instead promote microvesicle from plasma membrane. Additionally, ICP0 (Infected Cell Protein 0) enhances The different types can be separated by density gradients due their distinct biophysical properties. L-particles ESCRT+ display a pro-viral role, supporting replication, whereas exhibit antiviral effects. Overall, these studies highlight yields numerous diverse with type composition shaped ability evade responses. likely shape microenvironment determine disease outcomes.

Language: Английский

Citations

0
Infections, Genetics, and Alzheimer's Disease: Exploring the Pathogenic Factors for Innovative Therapies DOI

Ramesh Kordi,

T. John Andrews,

Mark D. Hicar

et al.

Virology, Journal Year: 2025, Volume and Issue: unknown, P. 110523 - 110523

Published: March 1, 2025

Language: Английский

Citations

0
Research on the mechanism and application of plant essential oils in improving Alzheimer’s disease DOI Creative Commons

Zhihao Wang,

Dong Yang,

Song Zhang

et al.

BIO Web of Conferences, Journal Year: 2024, Volume and Issue: 124, P. 02016 - 02016

Published: Jan. 1, 2024

Alzheimer’s disease (AD) is a neurodegenerative disease, mainly caused by brain cell aging, impaired autophagy function, and reduction of nicotinic acetylcholine receptors. Amyloid-β (Aβ) peptide aggregation Tau protein hyperphosphorylation, as well memory impairment cognitive dysfunction, are hallmarks the disease. Clinical current traditional anti-AD drugs have shortcomings such ineffective efficacy large side effects. Therefore, research new effective therapeutic has very broad space for development value significance. Plant essential oil (EO) obvious advantages in treatment diseases Because their lipophilicity, small molecular weight, easy to cross blood-brain barrier (BBB), rich active ingredients, they significant effects on regulating AD-related neurotransmitters hormone release, expression brain-derived neurotrophic factors pro-inflammatory cytokines. This article describes pathogenesis AD, summarizes recent studies anti-Alzheimer’s mechanisms oils, provides review AD chemical constituents plant oils. The theoretical basis oils alleviating treating

Language: Английский

Citations

0
Herpes simplex virus type 1 origin binding protein UL9 tethers and loops origin- and non-origin-DNA intra- and intermolecularly DOI Creative Commons
Anita F. Meier,

Joško Vučković,

Paul Girvan

et al.

Published: Oct. 30, 2024

Abstract Herpesviruses are ubiquitous human pathogens, which the causative agent of mild to severe symptoms ranging from cold sore nasopharyngeal carcinoma. Even though replication linear dsDNA genome has been studied for decades, we still lack a complete molecular understanding its mechanism. It proposed, but never shown directly, that HSV-1 origin binding protein UL9 binds two closely located sites within oriS sequence, thereby mediating looping, in turn facilitates initiation. Here, used an array single-molecule approaches test this long-standing hypothesis directly. Surprisingly, data show does not loop efficiently. However, demonstrate can form large DNA loops at non-origin sequences very efficiently, as well tether molecules intermolecularly. Contrary bending hypothesis, our findings indicate DNA, rather may play alternative role initiation, such tethering separate facilitate recombination.

Language: Английский

Citations

0