Eco-friendly biosynthesis of silver nanoparticles using marine-derived Fusarium exquisite: optimization, characterization, and evaluation of antimicrobial, antioxidant, and cytotoxic activities DOI Creative Commons
Sally A. Ali, Mohamed Osman, Eslam Mohamed

et al.

World Journal of Microbiology and Biotechnology, Journal Year: 2025, Volume and Issue: 41(5)

Published: May 1, 2025

Abstract Fungi serve as efficient biocatalysts for the eco-friendly synthesis of metal nanoparticles, yielding stable and bioactive nanomaterials. In this study, silver nanoparticles were synthesized (AgNPs) using marine-derived fungus Fusarium equiseti characterized them with various analytical methods. UV–Vis spectroscopy detected a surface plasmon resonance peak at 420 nm, confirming AgNP formation, while X-ray diffraction (XRD) verified their crystalline structure. Scanning electron microscopy (SEM) transmission (TEM) revealed spherical averaging 50 nm. FTIR analysis confirmed that fungal metabolites cap stabilize AgNPs. We optimized extracellular biosynthesis 30°C, pH 8, 2 mM AgNO₃ over 72 h. The marine-adapted F. was selected its robust metabolic capacity enzyme secretion, enhancing nanoparticle stability bioactivity. Biological assessments showed these AgNPs outperformed in antimicrobial activity, minimum inhibitory concentrations (MICs) 6.5 µg/mL against Staphylococcus aureus 7.5 Escherichia coli , plus antifungal effects on Candida albicans solani . They also displayed strong antioxidant activity (IC₅₀ = 56.98 µg/mL) cytotoxicity MCF-7 breast cancer cells 24.38 µg/mL). These enhanced likely stem from acting natural capping agents, minimizing aggregation boosting bioavailability biological interactions. Molecular docking studies reinforced results, revealing binding to microbial cell wall proteins, human apoptotic regulator Bcl-2 (an anticancer target), peroxiredoxin-5 (PRDX5). This green method provides sustainable, non-toxic alternative conventional chemical approaches, avoiding hazardous reagents delivering stable, multifunctional Future vivo validation biocompatibility are planned explore clinical pharmaceutical potential -derived Graphical

Language: Английский

Eco-friendly biosynthesis of silver nanoparticles using marine-derived Fusarium exquisite: optimization, characterization, and evaluation of antimicrobial, antioxidant, and cytotoxic activities DOI Creative Commons
Sally A. Ali, Mohamed Osman, Eslam Mohamed

et al.

World Journal of Microbiology and Biotechnology, Journal Year: 2025, Volume and Issue: 41(5)

Published: May 1, 2025

Abstract Fungi serve as efficient biocatalysts for the eco-friendly synthesis of metal nanoparticles, yielding stable and bioactive nanomaterials. In this study, silver nanoparticles were synthesized (AgNPs) using marine-derived fungus Fusarium equiseti characterized them with various analytical methods. UV–Vis spectroscopy detected a surface plasmon resonance peak at 420 nm, confirming AgNP formation, while X-ray diffraction (XRD) verified their crystalline structure. Scanning electron microscopy (SEM) transmission (TEM) revealed spherical averaging 50 nm. FTIR analysis confirmed that fungal metabolites cap stabilize AgNPs. We optimized extracellular biosynthesis 30°C, pH 8, 2 mM AgNO₃ over 72 h. The marine-adapted F. was selected its robust metabolic capacity enzyme secretion, enhancing nanoparticle stability bioactivity. Biological assessments showed these AgNPs outperformed in antimicrobial activity, minimum inhibitory concentrations (MICs) 6.5 µg/mL against Staphylococcus aureus 7.5 Escherichia coli , plus antifungal effects on Candida albicans solani . They also displayed strong antioxidant activity (IC₅₀ = 56.98 µg/mL) cytotoxicity MCF-7 breast cancer cells 24.38 µg/mL). These enhanced likely stem from acting natural capping agents, minimizing aggregation boosting bioavailability biological interactions. Molecular docking studies reinforced results, revealing binding to microbial cell wall proteins, human apoptotic regulator Bcl-2 (an anticancer target), peroxiredoxin-5 (PRDX5). This green method provides sustainable, non-toxic alternative conventional chemical approaches, avoiding hazardous reagents delivering stable, multifunctional Future vivo validation biocompatibility are planned explore clinical pharmaceutical potential -derived Graphical

Language: Английский

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