Recent Progress in Detection and Profiling of Cancer Cell‐Derived Exosomes DOI
Huiwen Xiong, Zhipeng Huang,

Zhejun Yang

et al.

Small, Journal Year: 2021, Volume and Issue: 17(35)

Published: June 2, 2021

Exosomes, known as nanometer-sized vesicles (30-200 nm), are secreted by many types of cells. Cancer-derived exosomes have great potential to be biomarkers for early clinical diagnosis and evaluation cancer therapeutic efficacy. Conventional detection methods limited low sensitivity reproducibility. There hundreds papers published with different in recent years address these challenges. Therefore, this review, pioneering researches about various strategies comprehensively summarized the analytical performance tests is evaluated. Furthermore, exosome molecular composition (protein nucleic acid) profiling, a single their application reviewed. Finally, principles applications machine learning method presented.

Language: Английский

Salivary Biomarkers for Oral Squamous Cell Carcinoma Diagnosis and Follow-Up: Current Status and Perspectives DOI Creative Commons
Marta Cristaldi, Rodolfo Mauceri, Olga Di Fede

et al.

Frontiers in Physiology, Journal Year: 2019, Volume and Issue: 10

Published: Dec. 10, 2019

Oral cancer is the sixth most common type in world, and 90% of it represented by oral squamous cell carcinoma (OSCC). Despite progress preventive therapeutic strategies, delay OSCC diagnosis remains one major causes high morbidity mortality; indeed majority has been lately identified advanced clinical stage (i.e., III or IV). Moreover, after primary treatment, recurrences and/or metastases are found more than half patients (80% cases within first 2 years) 5-year survival rate still lower 50%, resulting a serious issue for public health. Currently, histological investigation represents "gold standard" diagnosis; however, recent studies have evaluated potential use non-invasive methods, such as "liquid biopsy," detection diagnostic prognostic biomarkers body fluids patients. Saliva biofluid containing factors cytokines, DNA RNA molecules, circulating tissue-derived cells, extracellular vesicles (EVs) that may be used biomarkers; their analysis give us useful information to do early improve prognosis. Therefore, aim this review reporting data on saliva biomarker liquid biopsy from patients, with particular attention tumor (ctDNA), EVs, microRNAs (miRNAs). Our results highlight several promising uses management; painless, accessible, low cost very helpful source detection. Even if standardized protocols isolation, characterization, evaluation needed, suggest successfully included future processes, considerable impact treatment strategies favorable outcome.

Language: Английский

Citations

149

Exosomes as Naturally Occurring Vehicles for Delivery of Biopharmaceuticals: Insights from Drug Delivery to Clinical Perspectives DOI Creative Commons
Arun Butreddy, Nagavendra Kommineni,

Narendar Dudhipala

et al.

Nanomaterials, Journal Year: 2021, Volume and Issue: 11(6), P. 1481 - 1481

Published: June 3, 2021

Exosomes as nanosized vesicles are emerging drug delivery systems for therapeutics owing to their natural origin, ability mediate intercellular communication, and potential encapsulate various biological molecules such proteins nucleic acids within the lipid bilayer membrane or in lumen. contain endogenous components (proteins, lipids, RNA) that could be used deliver cargoes target cells, offering an opportunity diagnose treat diseases. Owing travel safely extracellular fluid transport cells with high efficacy, exosomes offer enhanced of vivo. However, several challenges related stabilization exosomes, production sufficient amounts safety efficient loading drugs into clearance from circulation, transition bench scale clinical may limit development use. For use it is important understand molecular mechanisms behind function exosome vesicles. This review exploits techniques isolation characterization enhance therapeutic outcome methods. Further, routes administration, trials, regulatory aspects will discussed this review.

Language: Английский

Citations

148

Electrochemical Biosensors for Whole Blood Analysis: Recent Progress, Challenges, and Future Perspectives DOI
Shaoguang Li, Hongyuan Zhang, Man Zhu

et al.

Chemical Reviews, Journal Year: 2023, Volume and Issue: 123(12), P. 7953 - 8039

Published: June 1, 2023

Whole blood, as one of the most significant biological fluids, provides critical information for health management and disease monitoring. Over past 10 years, advances in nanotechnology, microfluidics, biomarker research have spurred development powerful miniaturized diagnostic systems whole blood testing toward goal monitoring treatment. Among techniques employed whole-blood diagnostics, electrochemical biosensors, known to be rapid, sensitive, capable miniaturization, reagentless washing free, become a class emerging technology achieve target detection specifically directly complex media, e.g., or even living body. Here we are aiming provide comprehensive review summarize over decade sensors analysis. Further, address remaining challenges opportunities integrate sensing platforms.

Language: Английский

Citations

130

Exosomes in atherosclerosis: performers, bystanders, biomarkers, and therapeutic targets DOI Creative Commons
Chen Wang, Zhelong Li,

Yunnan Liu

et al.

Theranostics, Journal Year: 2021, Volume and Issue: 11(8), P. 3996 - 4010

Published: Jan. 1, 2021

Exosomes are nanosized lipid vesicles originating from the endosomal system that carry many macromolecules their parental cells and play important roles in intercellular communication. The functions underlying mechanisms of exosomes atherosclerosis have recently been intensively studied. In this review, we briefly introduce exosome biology then focus on advances atherosclerosis, specifically exosomal changes associated with cellular origins potential functional cargos, detailed impacts recipient cells. We also discuss as biomarkers drug carriers for managing atherosclerosis.

Language: Английский

Citations

126

Recent Progress in Detection and Profiling of Cancer Cell‐Derived Exosomes DOI
Huiwen Xiong, Zhipeng Huang,

Zhejun Yang

et al.

Small, Journal Year: 2021, Volume and Issue: 17(35)

Published: June 2, 2021

Exosomes, known as nanometer-sized vesicles (30-200 nm), are secreted by many types of cells. Cancer-derived exosomes have great potential to be biomarkers for early clinical diagnosis and evaluation cancer therapeutic efficacy. Conventional detection methods limited low sensitivity reproducibility. There hundreds papers published with different in recent years address these challenges. Therefore, this review, pioneering researches about various strategies comprehensively summarized the analytical performance tests is evaluated. Furthermore, exosome molecular composition (protein nucleic acid) profiling, a single their application reviewed. Finally, principles applications machine learning method presented.

Language: Английский

Citations

115