Effects of Univariate Stiffness and Degradation of DNA Hydrogels on the Transcriptomics of Neural Progenitor Cells

Journal of the American Chemical Society, Journal Year: 2023, Volume and Issue: 145(16), P. 8954 - 8964

Published: April 8, 2023

Mechanical interactions between cells and extracellular matrix (ECM) are critical for stem cell fate decision. Synthetic models of ECM, such as hydrogels, can be used to precisely manipulate the mechanical properties niche investigate how signals regulate behavior. However, it has long been a great challenge tune solely ECM-mimic hydrogels' since altering most materials is usually accompanied by chemical topological changes. Here, we employ DNA its enantiomers prepare series hydrogels with univariate stiffness regulation, which enables precise interpretation decision neural progenitor (NPCs) in three-dimensional environment. Using single-cell RNA sequencing techniques, Monocle pseudotime trajectory CellphoneDB analysis, demonstrate that hydrogel alone does not influence differentiation NPCs, but degradation enhances cell-cell possibly main reason. We also find ECM remodeling facilitates sense stimuli.

Language: Английский

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Programmable DNA Hydrogel Provides Suitable Microenvironment for Enhancing TSPCS Therapy in Healing of Tendinopathy

Small, Journal Year: 2023, Volume and Issue: 19(32)

Published: April 17, 2023

Abstract Tendon stem/progenitor cells (TSPCs) therapy is a promising strategy for enhancing cell matrix and collagen synthesis, regulating the metabolism of tendon microenvironment during injury repair. Nevertheless, barren gliding shear cause insufficient nutrition supply, damage, aggregation injected TSPCs around tissues, which severely hinders their clinical application in tendinopathy. In this study, delivery system developed by encapsulating within DNA hydrogel (TSPCs‐Gel) as offers an excellent artificial extracellular (ECM) providing proliferation protection against forces. This method restricts to tendons, significantly extending retention time. It also found that TSPCs‐Gel injections can promote healing rat tendinopathy vivo, where cross‐sectional area load failure injured tendons rats are improved compared free treatment group at 8 weeks. Furthermore, potential mechanism investigated RNA‐sequencing identify series gene signaling pathway targets further strategies. These findings suggest pathways using hydrogels ECMs protect TSPC therapy.

Language: Английский

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Bioengineered Silk Protein‐Based 3D In Vitro Models for Tissue Engineering and Drug Development: From Silk Matrix Properties to Biomedical Applications

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: 13(28)

Published: July 15, 2024

3D in vitro model has emerged as a valuable tool for studying tissue development, drug screening, and disease modeling. systems can accurately replicate microstructures physiological features, mirroring the vivo microenvironment departing from conventional 2D cell cultures. Various models utilizing biomacromolecules like collagen synthetic polymers have been developed to meet diverse research needs address complex challenges of contemporary research. Silk proteins, bearing structural functional similarities collagen, increasingly employed construct advanced systems, surpassing limitations This review examines silk proteins' composition, structure, properties, functions, elucidating their role models. Furthermore, recent advances biomedical applications involving silk-based organoid are discussed. In particular, unique attributes matrix constituents constructs highlighted, providing meticulous evaluation importance. Additionally, it outlines current hurdles complexities while contemplating future avenues, thereby paving way developing biomimetic protein-based microtissues.

Language: Английский

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Programming the Assembly of Oligo-Adenine with Coralyne into a pH-Responsive DNA Hydrogel

ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: 16(12), P. 15394 - 15404

Published: March 15, 2024

External stimuli-responsive DNA hydrogels present interesting platforms for drug loading and triggered release. Typically, molecules are encapsulated within three-dimensionally hybridized networks. However, the utilization of as cofactors to facilitate directed assembly strands into hydrogel frameworks their subsequent controlled release remains be explored. Herein, we introduce guided oligo-adenine (A-strand) an acidic pH-responsive using anticancer drug, coralyne (COR), a low-molecular-weight cofactor. At pH 7, COR orchestrates A-strand antiparallel duplex configuration cross-linked by A-COR-A units at stoichiometric ratio one cofactor per four adenine bases, resulting in characterized bridges. 4–5, instability results disintegration its constituent components, leading simultaneous dissociation matrix. This study introduces method which molecules, exemplified here COR, direct formation supramolecular cofactor-DNA complex, subsequently creation hydrogel. approach may inspire future investigations tailored encapsulation applications.

Language: Английский

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Convenient tumor 3D spheroid arrays manufacturing via acoustic excited bubbles for in situ drug screening

Lab on a Chip, Journal Year: 2023, Volume and Issue: 23(6), P. 1593 - 1602

Published: Jan. 1, 2023

Rapid construction of size-controllable 3D tumor spheroid arrays based on acoustic bubbles in microfluidic chips for situ drug response testing.

Language: Английский

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