Biomacromolecules,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 21, 2025
Sugar
beet
pectin,
an
anionic
polysaccharide,
and
silk
fibroin,
a
high
molecular
weight
protein,
undergo
gelation
through
ionic
interactions
conformational
changes,
leading
to
hydrogel
formation.
Although
many
studies
have
focused
on
bulk
gel
systems
involving
polysaccharides
proteins,
more
research
is
needed
investigate
their
properties
at
the
microscale
level.
In
this
context,
we
developed
microgel
system
based
pectin/fibroin
combination
investigated
its
properties.
We
two
mechanisms:
physical
cross-linking
enzymatic
covalent
cross-linking.
The
microgels
were
fabricated
using
droplet-based
microfluidics,
secondary
structure,
mechanical
properties,
degradation
profiles
investigated.
Our
experimental
results
show
that
exhibit
ordered
β-sheet
Young's
modulus
in
range
of
10
20
kPa,
can
be
promoted
protease
enzymes.
Finally,
biocompatibility
assessed
Alamar
Blue
cell
viability
assay
with
human
pulmonary
fibroblasts
(HPFs).
This
presents
highly
functional
hybrid
biomaterial
produced
from
waste
products
structural
demonstrating
compatibility
potential
tissue
engineering
applications.
Langmuir,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 19, 2025
Microgels,
which
are
submicrometer-
to
micrometer-sized
hydrogels,
have
been
investigated
for
more
than
four
decades
and
now
widely
applied
in
modern
advanced
smart
materials.
The
"smartness"
of
microgel-based
materials
is
attributed
their
material
composition,
cross-linking
strategy,
responsiveness
stimuli.
These
characteristics
inherently
influenced
constrained
by
the
fabrication
method,
which,
turn,
affects
properties
resulting
microgel
particles.
While
numerous
studies
reported
on
applications
microgels,
translation
fundamental
research
findings
into
practical
remains
limited.
For
example,
while
recent
biomedical
has
focused
controlled
drug
release
based
novel
environmentally
responsive
mechanisms,
this
Review
highlights
that
still
requires
further
refinement
terms
selectivity
precision.
Moreover,
variety
drugs
can
be
used
limited,
as
clarifies,
frequently
do
not
possess
adequate
biocompatibility
applications.
This
initially
summarizes
relationship
between
synthesis
techniques
properties.
Furthermore,
we
observe
reports
microgels
fall
primarily
categories
sensing,
separation,
applications,
additive
manufacturing.
highlight
advances
applications;
however,
several
challenges
specific
each
application
area
need
addressed.
instance,
improving
sensitivity
a
key
concern
sensing
field.
identifies
these
proposes
future
directions
advancement
Biomacromolecules,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 21, 2025
Sugar
beet
pectin,
an
anionic
polysaccharide,
and
silk
fibroin,
a
high
molecular
weight
protein,
undergo
gelation
through
ionic
interactions
conformational
changes,
leading
to
hydrogel
formation.
Although
many
studies
have
focused
on
bulk
gel
systems
involving
polysaccharides
proteins,
more
research
is
needed
investigate
their
properties
at
the
microscale
level.
In
this
context,
we
developed
microgel
system
based
pectin/fibroin
combination
investigated
its
properties.
We
two
mechanisms:
physical
cross-linking
enzymatic
covalent
cross-linking.
The
microgels
were
fabricated
using
droplet-based
microfluidics,
secondary
structure,
mechanical
properties,
degradation
profiles
investigated.
Our
experimental
results
show
that
exhibit
ordered
β-sheet
Young's
modulus
in
range
of
10
20
kPa,
can
be
promoted
protease
enzymes.
Finally,
biocompatibility
assessed
Alamar
Blue
cell
viability
assay
with
human
pulmonary
fibroblasts
(HPFs).
This
presents
highly
functional
hybrid
biomaterial
produced
from
waste
products
structural
demonstrating
compatibility
potential
tissue
engineering
applications.
