Protein Corona of Nanoparticles: Isolation and Analysis

Chem & Bio Engineering, Journal Year: 2024, Volume and Issue: 1(9), P. 757 - 772

Published: Oct. 3, 2024

Nanoparticles entering biological systems or fluids inevitably adsorb biomolecules, such as protein, on their surfaces, forming a protein corona. Ensuing, the corona endows nanoparticles with new identity and impacts interaction between systems. Hence, development of reliable techniques for isolation analysis is key understanding behaviors nanoparticles. First, this review systematically outlines approach isolating corona, including centrifugation, magnetic separation, size exclusion chromatography, flow-field-flow fractionation, other emerging methods. Next, we qualitative quantitative characterization methods Finally, underscore necessary steps to advance efficiency fidelity nanoparticle surfaces. We anticipate that these insights into methodologies will profoundly influence technologies aimed at elucidating bionano interactions role in various biomedical applications.

Language: Английский

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Deep Plasma Proteome Profiling by Modulating Single Nanoparticle Protein Corona with Small Molecules

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: March 8, 2024

The protein corona, a dynamic biomolecular layer that forms on nanoparticle (NP) surfaces upon exposure to biological fluids is emerging as valuable diagnostic tool for improving plasma proteome coverage analyzed by liquid chromatography-mass spectrometry (LC-MS/MS). Here, we show spiking small molecules, including metabolites, lipids, vitamins, and nutrients (namely, glucose, triglyceride, diglycerol, phosphatidylcholine, phosphatidylethanolamine, L-α-phosphatidylinositol, inosine 5′-monophosphate, B complex), into can induce diverse corona patterns otherwise identical NPs, significantly enhancing the depth of profiling. coronas polystyrene NPs when exposed treated with an array molecules (n=10) allowed detection 1793 proteins marking 8.25-fold increase in number quantified compared alone (218 proteins) 2.63-fold relative untreated (681 proteins). Furthermore, discovered adding 1000 µg/ml phosphatidylcholine could singularly enable 897 proteins. At this specific concentration, selectively depleted four most abundant proteins, albumin, thus reducing range enabling lower abundance. By employing optimized data-independent acquisition (DIA) approach, inclusion led 1436 single sample. Our molecular results revealed interacts albumin via hydrophobic interactions, h-bonds, water-bridges. Addition also enabled 337 additional proteoforms using top-down proteomics approach. These significant achievements are made utilizing only NP type one molecule analyze sample, setting new standard Given critical role biomarker discovery disease monitoring, anticipate widespread adoption methodology identification clinical translation proteomic biomarkers FDA approved diagnostics.

Language: Английский

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Perspectives on Protein–Nanoparticle Interactions at the In Vivo Level

Langmuir, Journal Year: 2024, Volume and Issue: 40(15), P. 7781 - 7790

Published: April 4, 2024

The distinct features of nanoparticles have provided a vast opportunity developing new diagnosis and therapy strategies for miscellaneous diseases. Although few nanomedicines are available in the market or translation stage, many important issues still unsolved. When entering body, nanomaterials will be quickly coated by proteins from their surroundings, forming corona on surface, so-called protein corona. Studies shown that has biological implications, particularly at vivo level. For example, they can promote immune system to rapidly clear these outer materials prevent playing designed role therapy. In this Perspective, techniques characterizing protein–nanoparticle interactions critically summarized. Effects nanoparticle properties environmental factors formation, which further regulate fate nanoparticles, highlighted discussed. Moreover, recent progress biomedical application corona-engineered is introduced, future directions yet challenging research area also briefly

Language: Английский

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Effect of Nanoparticle Curvature on Its Interaction with Serum Proteins

Langmuir, Journal Year: 2024, Volume and Issue: 40(29), P. 15205 - 15213

Published: July 11, 2024

The size or the curvature of nanoparticles (NPs) plays an important role in regulating composition protein corona. However, molecular mechanisms how affects interaction NPs with serum proteins still remain elusive. In this study, we employ all-atom dynamics simulations to investigate interactions between two typical and PEGylated Au three different surface curvatures (0, 0.1, 0.5 nm

Language: Английский

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Polyester nanoparticles delivering chemotherapeutics: Learning from the past and looking to the future to enhance their clinical impact in tumor therapy

Wiley Interdisciplinary Reviews Nanomedicine and Nanobiotechnology, Journal Year: 2024, Volume and Issue: 16(5)

Published: Sept. 1, 2024

Abstract Polymeric nanoparticles (NPs), specifically those comprised of biodegradable and biocompatible polyesters, have been heralded as a game‐changing drug delivery platform. In fact, poly(α‐hydroxy acids) such polylactide (PLA), poly(lactide‐ co ‐glycolide) (PLGA), poly(ε‐caprolactone) (PCL) heavily researched in the past three decades material basis polymeric NPs for applications. As materials, these polymers found success resorbable sutures, implants, even monolithic, platforms sustained release therapeutics (e.g., proteins small molecules) diagnostics. Few fields gained more attention through than cancer therapy. However, clinical translational nanomedicines treating solid tumors has not congruent with fervor or funding this particular field research. Here, we attempt to provide comprehensive snapshot polyester context chemotherapeutic delivery. This includes preliminary exploration nanomedicine research space. We examine various processes producing NPs, including methods surface‐functionalization, related challenges. After detailed overview multiple factors involved tumors, crosstalk between particle design interactions biological systems is discussed. Finally, report state‐of‐the‐art approaches toward effective aiming at identifying new areas re‐evaluating reasons why some avenues underdelivered. hope our effort will contribute better understanding gap fill delineate future work needed bring polyester‐based closer application. article categorized under: Therapeutic Approaches Drug Discovery > Nanomedicine Oncologic Disease Nanotechnology Biology Nanoscale Systems Emerging Technologies

Language: Английский

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