International Journal of Molecular Sciences,
Journal Year:
2024,
Volume and Issue:
25(24), P. 13540 - 13540
Published: Dec. 18, 2024
Gene
transfection
is
a
fundamental
technique
in
the
fields
of
biological
research
and
therapeutic
innovation.
Due
to
their
biocompatibility
membrane-mimetic
properties,
lipid
vectors
serve
as
essential
tools
transfection.
The
successful
delivery
genetic
material
into
cytoplasm
contingent
upon
fusion
vector
cellular
membranes,
which
enables
hydrophilic
polynucleic
acids
traverse
hydrophobic
barriers
two
intervening
membranes.
This
review
examines
critical
role
membrane
lipofection
efficiency,
with
particular
focus
on
molecular
mechanisms
that
govern
lipoplex–membrane
interactions.
analysis
will
examine
key
challenges
inherent
process,
from
achieving
initial
proximity
facilitating
final
content
release
through
remodeling.
In
contrast
viral
vectors,
utilize
specialized
proteins,
necessitate
strategic
formulation
environmental
optimization
enhance
fusogenicity.
discusses
recent
advances
design
fusion-promoting
strategies,
emphasizing
potential
improve
gene
yield.
It
highlights
importance
understanding
for
developing
next-generation
systems
emphasizes
need
continued
advance
lipid-mediated
technology.
ACS Nano,
Journal Year:
2024,
Volume and Issue:
18(29), P. 19283 - 19302
Published: July 11, 2024
Developing
strategies
to
target
injured
pancreatic
acinar
cells
(PACs)
in
conjunction
with
primary
pathophysiology-specific
pharmacological
therapy
presents
a
challenge
the
management
of
acute
pancreatitis
(AP).
We
designed
and
synthesized
trypsin-cleavable
organosilica
precursor
bridged
by
arginine-based
amide
bonds,
leveraging
trypsin's
ability
selectively
identify
guanidino
groups
on
arginine
via
Asp189
at
active
S1
pocket
cleave
carboxy-terminal
(C-terminal)
bond
catalytic
triads.
The
precursors
were
incorporated
into
framework
mesoporous
silica
nanoparticles
(MSNs)
for
encapsulating
membrane-permeable
Ca
Advanced Functional Materials,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 21, 2025
Abstract
Bone
defects
remain
challenging
to
treat,
with
common
therapies
still
relying
on
invasive
approaches.
Mesenchymal
stem
cell‐derived
extracellular
vesicles
(MSC‐EVs)
offer
a
promising
alternative
due
their
regenerative
and
immunomodulatory
properties,
but
challenges
in
scalable
EV
production
limit
clinical
translation.
Nanoghosts
(NGs)
are
an
emerging
class
of
EV‐mimetics
synthesized
through
the
physical
distortion
ghost
cells
that
innate
bioactivity
similar
EVs
while
having
more
yields.
In
this
study,
osteogenic
potential
NGs
made
from
MSC
ghosts
(MSC‐NGs)
is
explored
for
first
time
contrasted
conventional
MSC‐EVs.
MSC‐NGs
generated
sonication,
yielding
two‐fold
compared
MSC‐EVs
same
number
cells.
Unlike
MSC‐EVs,
significantly
enhanced
differentiation
MSCs,
evidenced
by
increased
alkaline
phosphatase
(ALP)
activity
early
mineralization.
Proteomic
analysis
further
revealed
enriched
osteogenesis‐related
proteins
than
vivo,
treatment
0.5
mm
mouse
femoral
osteotomy
accelerated
fracture
healing,
showing
callus
mineralization
day
14
improved
bone
marrow
reconstitution
21,
along
reduced
osteoclastic
activity.
These
findings
demonstrate
as
effective
therapeutics
tissue
engineering,
offering
advantages
over
future
healing
strategies.
Small Methods,
Journal Year:
2024,
Volume and Issue:
unknown
Published: March 10, 2024
Abstract
Although
there
are
various
advancements
in
biomedical
the
past
few
decades,
still
challenges
treatment
of
brain
diseases.
The
main
difficulties
inability
to
deliver
a
therapeutic
dose
drug
through
blood‐brain
barrier
(BBB)
and
serious
side
effects
drug.
Thus,
it
is
essential
select
biocompatible
carriers
novel
tools
better
enhance
effect
disease
treatment.
In
recent
years,
biomimetic
nanoparticles
(BNPs)
based
on
natural
cell
membranes,
which
have
excellent
biocompatibility
low
immunogenicity,
widely
used
diseases
enable
successfully
cross
BBB
target
lesions.
BNPs
can
prolong
circulation
time
vivo,
more
conducive
aggregation
Cell
membranes
(CMs)
from
cancer
cells
(CCs),
red
blood
(RBCs),
white
(WBCs),
so
as
coatings
for
(NPs)
achieve
ability
target,
evade
clearance,
or
stimulate
immune
system.
This
review
summarizes
application
different
sources
discusses
possibilities
clinical
translation.
Journal of Extracellular Vesicles,
Journal Year:
2024,
Volume and Issue:
13(7)
Published: June 28, 2024
Abstract
Extracellular
vesicles
have
gained
wide
momentum
as
potential
therapeutics
for
osteoarthritis,
a
highly
prevalent
chronic
disease
that
still
lacks
an
approved
treatment.
The
membrane‐bound
are
secreted
by
all
cells
carrying
different
cargos
can
serve
both
biomarkers
and
modifiers.
Nonetheless,
despite
significant
peak
in
research
regarding
EVs
OA
therapeutics,
clinical
implementation
seems
distant.
In
addition
to
scalability
standardization
challenges,
researchers
often
omit
focus
on
consider
the
proper
tropism
of
vesicles,
practicality
relevance
their
source,
low
native
therapeutic
efficacy,
whether
they
address
whole.
These
considerations
necessary
better
understand
light
been
comprehensively
discussed
ultimately
summarized
this
review
into
conceptualized
framework
termed
nanodiamond
concept.
Future
perspectives
also
discussed,
alternatives
presented
some
challenges
concerns.
Journal of Nanobiotechnology,
Journal Year:
2024,
Volume and Issue:
22(1)
Published: June 25, 2024
Abstract
Parkinson’s
disease
(PD)
is
the
second
largest
group
of
neurodegenerative
diseases,
and
its
existing
drug
treatments
are
not
satisfactory.
Natural
cell
membrane
drugs
used
for
homologous
targeting
to
enhance
efficacy.
In
this
study,
microfluidic
electroporation
chip
prepared
mesenchymal
stem
cell-derived
neuron-like
membrane-coated
curcumin
PLGA
nanoparticles
(MM-Cur-NPs)
was
synthesized
explored
therapeutic
effect
mechanism
in
PD.
MM-Cur-NPs
can
protect
neuron
from
damage,
restore
mitochondrial
potential
reduce
oxidative
stress
vitro.
PD
mice,
it
also
improve
movement
disorders
damaged
TH
neurons.
found
be
distributed
brain
metabolized
with
a
delay
within
24
h.
After
1
h
administration,
were
variety
neurotransmitters
significantly
upregulated,
such
as
dopamine.
Differentially
expressed
genes
RNA-seq
enriched
inflammation
regulation,
up-expression
anti-inflammatory
factors
inhibited
pro-inflammatory
Mechanically,
only
neuronal
apoptosis,
inhibit
microglial
marker
IBA-1
inflammation,
but
upregulate
expression
protein
VDAC1
potential.
This
study
proposes
strategy
provide
neuroprotective
effects
through
therapy
Journal of Nanobiotechnology,
Journal Year:
2025,
Volume and Issue:
23(1)
Published: Jan. 29, 2025
Acute
pancreatitis
(AP)
is
a
disease
characterized
by
an
acute
inflammatory
response
in
the
pancreas.
This
caused
abnormal
activation
of
pancreatic
enzymes
variety
etiologic
factors,
which
results
localized
response.
The
symptoms
this
include
abdominal
pain,
nausea
and
vomiting
fever.
These
are
induced
hyperinflammatory
oxidative
stress.
In
recent
years,
research
has
focused
on
developing
anti-inflammatory
antioxidative
therapies
for
treatment
(AP).
However,
there
still
limitations
to
approach,
including
poor
drug
stability,
low
bioavailability
short
half-life.
advent
nanotechnology
opened
up
novel
avenue
management
Nanomaterials
can
serve
as
efficacious
vehicle
conventional
pharmaceuticals,
enhancing
their
targeting
ability,
improving
prolonging
Moreover,
they
also
exert
direct
therapeutic
effect.
review
begins
introducing
general
situation
It
then
discusses
pathogenesis
current
status
treatment.
Finally,
it
considers
literature
related
nanomaterials.
objective
study
provide
comprehensive
existing
use
nanomaterials
particular,
changes
markers
outcomes
following
administration
examined.
done
with
intention
offering
insights
that
inform
subsequent
facilitate
clinical
application
