Early nanoparticle intervention preserves motor function following cervical spinal cord injury

Bioengineering & Translational Medicine, Journal Year: 2025, Volume and Issue: unknown

Published: April 29, 2025

Abstract Spinal cord injury (SCI) triggers an immediate influx of immune cells that secrete pro‐inflammatory cytokines and reactive oxygen species cause tissue damage is secondary to the initial physical trauma. We aim reprogram these promote a less inflammatory more pro‐regenerative environment. Herein, we investigated window in time during which poly(lactide‐co‐glycolide) nanoparticles (NPs) administration can successfully modulate response functional sparing. The dynamics cell infiltration were studied following injection NPs intravenously every 24 h for 7 days injury, with first starting at 2, 4, or hours post‐injury (hpi). At (dpi), early NP intervention decreased number infiltrating macrophages neutrophils, but delaying treatment until hpi increased neutrophils above control. All mice received had greater neuronal sparing contralateral timepoints neuromuscular junction innervation motor endplate neurons neural circuits 2 group corresponded function, as measured by ladder beam test. Collectively, results suggest preserve function SCI.

Language: Английский

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Harnessing nanomedicine for modulating microglial states in the central nervous system disorders: Challenges and opportunities

Biomedicine & Pharmacotherapy, Journal Year: 2024, Volume and Issue: 177, P. 117011 - 117011

Published: June 24, 2024

Microglia are essential for maintaining homeostasis and responding to pathological events in the central nervous system (CNS). Their dynamic multidimensional states different environments pivotal factors various CNS disorders. However, therapeutic modulation of microglial is challenging due intricate balance these cells maintain environment blood-brain barrier's restriction drug delivery. Nanomedicine presents a promising avenue addressing challenges, offering method targeted efficient states. This review covers challenges faced potential use nanoparticle-based delivery systems. We provide an in-depth examination nanoparticle applications modulating range disorders, encompassing neurodegenerative autoimmune diseases, infections, traumatic injuries, stroke, tumors, chronic pain, psychiatric conditions. highlights recent advancements future prospects nanomedicine modulation, paving way research clinical interventions

Language: Английский

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GsMTx4 ameliorates spinal cord injury by regulating microglial polarization through the Piezo1/NFκB/STAT6 pathway

Journal of Neurorestoratology, Journal Year: 2024, Volume and Issue: 12(4), P. 100144 - 100144

Published: Aug. 16, 2024

Language: Английский

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C–C motif chemokine ligand 2/C–C motif chemokine receptor 2 pathway as a therapeutic target and regulatory mechanism for spinal cord injury

Neural Regeneration Research, Journal Year: 2024, Volume and Issue: 20(8), P. 2231 - 2244

Published: July 29, 2024

Spinal cord injury involves non-reversible damage to the central nervous system that is characterized by limited regenerative capacity and secondary inflammatory damage. The expression of C-C motif chemokine ligand 2/C-C receptor 2 axis exhibits significant differences before after injury. Recent studies have revealed closely associated with responses recruitment immune cells following spinal injury, suggesting this a novel target regulatory control point for treatment. This review comprehensively examines therapeutic strategies targeting axis, along repair mechanisms linking Additionally, we summarize upstream downstream signaling pathways axis. primarily elaborates on latest progress research antagonistic drugs, approaches used exploit new targets within development targeted drugs. Nevertheless, there are presently no clinical relating focusing aims provide ideas future treatment

Language: Английский

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Macrophage Membrane Spontaneously Encapsulated Cyclodextrin-Based Nanomedicines for Improving Lipid Metabolism and Inflammation in Atherosclerosis

ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: 16(37), P. 49660 - 49672

Published: Sept. 6, 2024

Atherosclerosis is a persistent inflammatory condition of the blood vessels associated with abnormalities in lipid metabolism. Development biomimetic nanoplatforms provides an effective strategy. Herein, inspired by peptide CLIKKPF spontaneously coupling to phosphatidylserine (PS) on inner leaflet cell membranes specifically, MM@NPs were constructed macrophage membrane spontaneous encapsulation cyclodextrin-based nanoparticles modified and loaded hydrophobic compound resveratrol. could be specifically phagocytized activated endothelium overexpressed VCAM-1 for enhancing target delivery into pathological lesion. Additionally, ApoE

Language: Английский

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Tannic Acid-Based Biomimetic Nanomedicine with Pathological Reactive Oxygen Species-Responsive Cargo Release for Relieving Inflammation in Rheumatoid Arthritis

ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: 16(44), P. 59789 - 59802

Published: Oct. 25, 2024

Rheumatoid arthritis (RA) is a chronic disease characterized by immune cell infiltration and cartilage damage. The local lesion of RA shows severe oxidative stress proinflammatory cytokine secretion. For drug therapy, the efficacy agents, such as methotrexate (MTX), may be greatly limited, resulting from low bioavailability, clearance, toxic side effects. A nanocarrier (TA-PBA NPs) was developed with anti-inflammatory antioxidant activities, combined MTX to prepare nanomedicine (MTX for synergistic treatment RA. Moreover, inspired biological functions homing inflammation macrophages, biomimetic camouflaged macrophage membrane (MM@MTX constructed. TA-PBA NPs could timely promote release in response overaccumulated ROS exhibit high activities alleviating progression. experimental results confirmed that MM@MTX significantly reduce secretion cytokines (TNF-α) while increasing typical (IL-10), phenotype transformation macrophages M1 M2, up-regulate Nrf2-keap1 pathway-related proteins (HO-1 NRF2) positively regulate effectively inhibiting development. Thus, represent possible candidate safe efficient nanotherapy platform management.

Language: Английский

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Regulation of dynamic spatiotemporal inflammation by nanomaterials in spinal cord injury

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: Dec. 19, 2024

Spinal cord injury (SCI) is a common clinical condition of the central nervous system that can lead to sensory and motor impairment below level or permanent loss function in severe cases. Dynamic spatiotemporal neuroinflammation vital neurological recovery, which collectively constituted by dynamic changes series inflammatory cells, including microglia, neutrophils, astrocytes, among others. Immunomodulatory nanomaterials readily improve therapeutic effects simultaneously overcome various drawbacks associated with treatment, such as off-target side bioactivity immune agents during circulation. In this review, we discuss role inflammation secondary injuries after SCI, elaborate on mechanism action effect existing treating summarize mechanism(s) whereby they regulate inflammation. Finally, challenges prospects using nanotechnology modulate immunotherapy are discussed provide new insights for future treatment. Deciphering intricate mechanisms SCI requires further in-depth studies. Therefore, continues represent formidable challenge.

Language: Английский

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Identification of candidate biomarkers and pathways associated with multiple sclerosis using bioinformatics and next generation sequencing data analysis

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Dec. 7, 2023

Abstract Multiple sclerosis (MS) is the main reason for disability caused by autoimmunity. However, effective biomarkers MS have not been identified. This study explored molecular mechanisms and potential therapeutic targets occurrence progression using bioinformatics next generation sequencing (NGS) data analysis. NGS GSE138614, including patients with 25 normal control samples, were obtained from Gene Expression Omnibus (GEO) database Differentially expressed genes (DEGs) filtered subjected to gene ontology (GO) pathway enrichment analyses. Protein–protein interaction (PPI) network module analyses performed based on DEGs. MiRNA-hub regulatory TF-hub analysis built Cytoscape predict underlying microRNAs (miRNAs) transcription factors (TFs) associated hub genes. We also validated identified via receiver operating characteristic (ROC) curve A total of 959 DEGs (479 up regulated 480 down genes) detected. The GO terms pathways involve immune system process, developmental regulation cholesterol biosynthesis SREBP (SREBF). revealed that include LCK, PYHIN1, SLAMF1, DOK2, TAB2, CFTR, RHOB, LMNA, EGLN3 ERBB3 might be involved in development MS. present investigation illustrates a profile MS, which contribute interpretation provide novel

Language: Английский

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Nanosystems-enabled regenerative strategies for spinal cord Injury: Recent advances and future prospects

Materials & Design, Journal Year: 2023, Volume and Issue: 237, P. 112617 - 112617

Published: Dec. 30, 2023

Spinal cord injury (SCI) is a debilitating condition with limited treatment options, necessitating the exploration of innovative regenerative approaches. Nanosystems have emerged as promising tools for neural tissue engineering and drug delivery in SCI. This review provides concise overview current progress future directions nanosystems-enabled therapies It highlights underlying mechanisms SCI, including pathophysiology, inflammatory response, axonal degeneration. Various nanosystems, such nanoparticles nano-scaffolds, are discussed their potential promoting regeneration, neuroprotection, controlled delivery. Additionally, challenges clinical translation, safety considerations, regulatory aspects addressed, along assessment biocompatibility adverse effects. immense nanosystems SCI emphasizes need further research to overcome remaining hurdles facilitate implementation.

Language: Английский

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Prussian blue nanotechnology in the treatment of spinal cord injury: application and challenges

Frontiers in Bioengineering and Biotechnology, Journal Year: 2024, Volume and Issue: 12

Published: Sept. 11, 2024

Spinal cord injury (SCI) is a serious neurological condition that currently lacks effective treatments, placing heavy burden on both patients and society. Prussian blue nanoparticles exhibit great potential for treating spinal injuries due to their excellent physicochemical properties biocompatibility. These have strong anti-inflammatory antioxidant capabilities, effectively scavenge free radicals, reduce oxidative stress damage cells. nanotechnology shows broad application in drug delivery, bioimaging, cancer therapy, treatment, biosensors. This article reviewed the applications of injuries, explored challenges solutions associated with its application, discussed future prospects this technology SCI treatment.

Language: Английский

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