Biological carbon fixation benefits evaluation model construction and application based on atomic economy concept

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: March 3, 2025

Current methods for evaluating biocarbon fixation efficiency (BCF), such as genome-scale metabolic models and life cycle assessment, lack consideration of reaction atom economy fail to connect genetic relationships with the process. To address these limitations, we introduced an atomic evaluation index centered on enzyme kinetics, named Economic Indicators Real Biological Carbon Fixation Atoms (EIRCBFA), proposed a machine learning-based model assess BCF at both conditions protein levels. Using gradient boosting, achieved R² values 0.853 0.937, respectively, in five-fold cross-validation. The was validated by optimizing dihydroxyacetone (DHA) biosynthesis, where predictions were consistent traditional carbon trends. Notably, highest EIRCBFA mutant, FLS_F484E, produced 33.19 mg/L DHA, yield three times that wild-type enzyme. RAEKP provides valuable tool pathways their true fixed economy.

Language: Английский

Rapid Assembly of Ultrafine Palladium Nanoparticle-Decorated HOF-101 Triggered by Guest Enzyme Encapsulation

Inorganic Chemistry, Journal Year: 2024, Volume and Issue: 63(45), P. 21607 - 21616

Published: Oct. 29, 2024

Rapid enzyme immobilization is essential for catalysis and sensing applications, yet constructing effective systems challenging due to the need balance activity with properties of surrounding framework. Herein, taking glucose oxidase (GOx) as a model, rapid straightforward approach was presented synthesizing palladium nanoparticles (PdNPs)-decorated GOx encapsulated in HOF-101 nanocomposite materials (designated PdNPs/GOx@HOF-101) through an situ photoreduction enzyme-triggering encapsulation. The enzyme's surface residues trigger nucleation around it hydrogen-bonded bio interface, completing self-assembly 0.5 h. Furthermore, biocomposites loaded ultrafine PdNPs show satisfactory photoelectrochemical (PEC) properties. As proof-of-concept, PEC biosensor constructed by utilizing PdNPs/GOx@HOF-101 photoactive probe, which can quickly sensitively detect simultaneously remain stable within circumstance 30-60 °C pH 4-8. These attributes pave way diverse including improved techniques, advanced biosensors, more efficient biocatalytic processes.

Language: Английский

Advancements in Biological Conversion of C1 Feedstocks: Sustainable Bioproduction and Environmental Solutions

ACS Synthetic Biology, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 29, 2024

The use of one-carbon (C1) feedstocks, including carbon dioxide (CO

Language: Английский