Engineered M13-Derived Bacteriophages Capable of Gold Nanoparticle Synthesis and Nanogold Manipulations DOI Open Access
Joanna Karczewska-Golec, Kamila Sadowska, Piotr Golec

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(20), P. 11222 - 11222

Published: Oct. 18, 2024

For years, gold nanoparticles (AuNPs) have been widely used in medicine and industry. Although various experimental procedures reported for their preparation manipulation, none of them is optimal all purposes. In this work, we engineered the N-terminus pIII minor coat protein bacteriophage (phage) M13 to expose a novel HLYLNTASTHLG peptide that effectively specifically binds gold. addition binding gold, phage could synthesize spherical AuNPs 20 nm other sizes depending on reaction conditions, aggregate them, precipitate from colloid, as revealed by transmission electron microscopy (TEM), atomic force (AFM), scanning (SEM), well ultraviolet–visible (UV–vis) Fourier-transform infrared (FTIR) spectroscopic methods. We demonstrated exposing foreign selected phage-displayed library may serve sustainable molecular factory both synthesis subsequent overnight ions at room temperature neutral pH absence strong reducing agents, such commonly NaBH4. Taken together, results suggest potential applicability new, vitro-identified gold-binding diverse biomimetic manipulations.

Language: Английский

Characterizing the hard and soft nanoparticle-protein corona with multilayer adsorption DOI Creative Commons
Oriol Vilanova, Alberto Martinez‐Serra, Marco P. Monopoli

et al.

Frontiers in Nanotechnology, Journal Year: 2025, Volume and Issue: 6

Published: Jan. 17, 2025

Nanoparticles (NPs) in contact with biological fluid adsorb biomolecules into a corona. This corona comprises proteins that strongly bind to the NP (hard corona) and loosely bound (soft dynamically exchange surrounding solution. While kinetics of hard formation is relatively well understood, thanks experiments robust simulation models, experimental characterization soft present more significant challenge. Here, we review current state art introduce novel open-source computational model simulate its dynamic behavior, for which provide documentation. We focus on case transferrin (Tf) interacting polystyrene NPs as an illustrative example, demonstrating how this captures complexities offers deeper insights structure behavior. show dominated by glassy evolution relate crowding effects. work advances our understanding corona, bridging limitations improved techniques.

Language: Английский

Citations

1
Nanotechnology for vision restoration DOI
Guglielmo Lanzani, Greta Chiaravalli, Elisabetta Colombo

et al.

Nature Reviews Bioengineering, Journal Year: 2024, Volume and Issue: 2(10), P. 829 - 848

Published: July 18, 2024

Language: Английский

Citations

4
Protein Corona of Nanoparticles: Isolation and Analysis DOI Creative Commons

Yinuo Sun,

Yaxin Zhou, Mubashar Rehman

et al.

Chem & Bio Engineering, Journal Year: 2024, Volume and Issue: 1(9), P. 757 - 772

Published: Oct. 3, 2024

Nanoparticles entering biological systems or fluids inevitably adsorb biomolecules, such as protein, on their surfaces, forming a protein corona. Ensuing, the corona endows nanoparticles with new identity and impacts interaction between systems. Hence, development of reliable techniques for isolation analysis is key understanding behaviors nanoparticles. First, this review systematically outlines approach isolating corona, including centrifugation, magnetic separation, size exclusion chromatography, flow-field-flow fractionation, other emerging methods. Next, we qualitative quantitative characterization methods Finally, underscore necessary steps to advance efficiency fidelity nanoparticle surfaces. We anticipate that these insights into methodologies will profoundly influence technologies aimed at elucidating bionano interactions role in various biomedical applications.

Language: Английский

Citations

4
Extreme Tolerance of Nanoparticle‐Protein Corona to Ultra‐High Abundance Proteins Enhances the Depth of Serum Proteomics DOI Creative Commons

Qiqi Liu,

Mengjie Wang,

Xin Dai

et al.

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 22, 2025

Abstract The serum nanoparticle‐protein corona (NPC) provides specific disease information, thus opening a new avenue for high‐throughput in‐depth proteomics to facilitate biomarker discovery. Yet, little is known about the interactions between NPs and proteins, its role in enhanced depth of proteomics. Herein, series protein spike‐in experiments are conducted systematically investigate depletion enrichment during NPC formation. Proteomic concentration‐dependent, exhibits powerful tolerance ultra‐high abundant proteins. In addition, protein‐protein (PPI), especially those involving albumin, play pivotal promoting proteomic depth. Furthermore, triple‐protein assay established interrogate relationship binding affinity concentration. formation product balancing affinity, concentration, PPI. Overall, this study elucidates how achieve gain better understanding as an essential tool proteome profiling.

Language: Английский

Citations

0
The impact of macrophage phenotype and heterogeneity on the total internalized gold nanoparticle counts DOI Creative Commons
Henry Lee, Dimitri Vanhecke, Sandor Balog

et al.

Nanoscale Advances, Journal Year: 2024, Volume and Issue: 6(18), P. 4572 - 4582

Published: Jan. 1, 2024

Multimodal analysis and quantification of gold nanoparticles at the single-cell level using FIB-SEM tomography in human monocyte-derived macrophages revealed phenotype-dependent behaviors influencing distinct kinetics heterogeneity uptake.

Language: Английский

Citations

2
Matrix-Insensitive Sensor Arrays via Peptide-Coated Nanoparticles: Rapid Saliva Screening for Pathogens in Oral and Respiratory Diseases DOI
Benjamin Lam, Lubna Amer, Elizabeth Thompson

et al.

ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 26, 2024

Plasmonic nanoparticle-based biosensors often report a colorimetric signal through the aggregation or clustering of nanoparticles (NPs), but these mechanisms typically struggle to function in complex biofluids. Here, we matrix-insensitive sensor array approach detect bacteria, fungi, and viruses whose is based on dissociation peptide-aggregated NPs by thiolated polyethylene glycol (HS-PEG) polymers. We show that HS-PEGs differing sizes have varying capabilities dissociate citrate-capped gold nanoparticle (AuNP) silver (AgNP) assemblies. The dissociative abilities were used this discriminate at 90% confidence level microorganisms

Language: Английский

Citations

2
Nano‐Biointeractions of Functional Nanomaterials: The Emerging Role of Inter‐Organelle Contact Sites, Targeting, and Signaling DOI Creative Commons
Naym Blal, Giuseppe Bardi, Pier Paolo Pompa

et al.

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: unknown

Published: June 29, 2024

Abstract The study of nano‐biointeractions, at the forefront interdisciplinary research, unveils intricate interplays between nanomaterials (NMs) and intracellular organelles, which are pivotal hubs orchestrating diverse cellular processes. Thanks also to formation dynamic contacts among their membranes, organelles regulate lipid exchange, calcium signaling, metabolic pathways. Recently, potential role NMs in homeostasis through regulation organelle membrane contact sites (MCSs) is emerging, a complete overview this issue still lacking. This perspective aims elucidating synergy functional site underscoring advancing comprehension cell biology mechanisms fostering therapeutic breakthroughs. subject represents crucial aspect as it can reveal new molecular targets for potentially revolutionize strategies. Nanotechnology may offer unprecedented tools decipher manipulate interfaces with remarkable precision. Engineered serve versatile probes effectors, enabling targeted modulation unraveling intricacies governing dynamics. Furthermore, nano‐biointeraction‐driven insights hold promise innovations, offering novel avenues diseases linked dysregulated contacts.

Language: Английский

Citations

1
Biomimetic Plasmonic Nanophages by Head/Tail Self-Assembling: Gold Nanoparticle/Virus Interactions DOI
Hazem Ahmed, H. F. López,

Francesco Boselli

et al.

ACS Nano, Journal Year: 2024, Volume and Issue: 18(32), P. 21302 - 21315

Published: July 31, 2024

Gold nanoparticles (AuNPs), because of their dual plasmonic and catalytic functionalities, are among the most promising nanomaterials for development therapeutic diagnostic tools severe diseases such as cancer neurodegeneration. Bacteriophages, massively present in human biofluids, emerging revolutionary biotechnological they can be engineered to display multiple specific binding moieties, providing effective targeting ability, high stability, low cost, sustainable production. Coupling AuNPs with phages lead an advanced generation nanotools great potential biomedical applications. In study, we analyzed interactions between differently sized filamentous M13 phages, establishing characterization platform that combines analytical techniques computational models in-depth understanding these hybrid self-assembling systems. A precise structurally interaction AuNP–M13 complexes was observed, leading a peculiar head/tail "tadpole-like" configuration. silico simulations allowed explaining mechanisms underlying preferential assembly route information about AuNPs' size-dependent interplay capsid proteins. The structures were proven biomimetic, eluding formation biomolecular corona. By keeping biological identity virion, nanostructures maintained natural recognition/targeting ability even presence crowding. addition, able tune nanostructures' tropism toward E. coli based on AuNP size. Overall, our results set fundamental basis standard workflow phage-based nanotools, valuable wide spectrum nanotechnology

Language: Английский

Citations

1
Engineered M13-Derived Bacteriophages Capable of Gold Nanoparticle Synthesis and Nanogold Manipulations DOI Open Access
Joanna Karczewska-Golec, Kamila Sadowska, Piotr Golec

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(20), P. 11222 - 11222

Published: Oct. 18, 2024

For years, gold nanoparticles (AuNPs) have been widely used in medicine and industry. Although various experimental procedures reported for their preparation manipulation, none of them is optimal all purposes. In this work, we engineered the N-terminus pIII minor coat protein bacteriophage (phage) M13 to expose a novel HLYLNTASTHLG peptide that effectively specifically binds gold. addition binding gold, phage could synthesize spherical AuNPs 20 nm other sizes depending on reaction conditions, aggregate them, precipitate from colloid, as revealed by transmission electron microscopy (TEM), atomic force (AFM), scanning (SEM), well ultraviolet–visible (UV–vis) Fourier-transform infrared (FTIR) spectroscopic methods. We demonstrated exposing foreign selected phage-displayed library may serve sustainable molecular factory both synthesis subsequent overnight ions at room temperature neutral pH absence strong reducing agents, such commonly NaBH4. Taken together, results suggest potential applicability new, vitro-identified gold-binding diverse biomimetic manipulations.

Language: Английский

Citations

0