Evaluation of Cancer Cell Invasion Ability Based on Electrochemical Impedance

Biosensors, Journal Year: 2025, Volume and Issue: 15(2), P. 91 - 91

Published: Feb. 6, 2025

Cancer metastasis is the leading cause of cancer-related deaths, with ability cancer cells to invade blood vessels or lymphatic systems, determining their metastatic potential. Therefore, rapid and accurate assessment cell invasiveness crucial. Current methods, such as Transwell assay fluorescent labeling, are complex, invasive, may disrupt physiological state live cells. In this study, we introduce an electrochemical impedance-based method for evaluating invasiveness, combining microfluidic technologies monitor invasion process in a dynamic environment. A stable chip 30 μm interdigital electrodes was developed, optimized HeLa detection. We identified 1 kHz optimal frequency achieving maximum impedance resolution invasiveness. By correlating response Zcells/Zno-cells established reliable model. This model validated hydrogen peroxide cytotoxicity assay, showing high correlation optical staining minimal error 1.89%, underscoring its potential drug efficacy prediction. The proposed offers detection, low cost, requires no manual intervention, making it efficient tool assessing therapeutic research.

Language: Английский

Precision Spatial Control of Tumor‐Stroma Interactions in Cancer Models via 3D Bioprinting for Advanced Research and Therapy

Advanced Functional Materials, Journal Year: 2025, Volume and Issue: unknown

Published: April 24, 2025

Abstract As an emerging technology for modeling cancer tissue in vitro, 3D bioprinting facilitates precise spatial manipulation of cells and biomaterials, allowing the replication complex architectures accurate recapitulation tumor microenvironment (TME) features. This review initially elucidates mechanisms underlying tumorigenesis associated TME complexity, with a particular focus on roles stromal cell populations characteristics extracellular matrix (ECM) progression. Moreover, this work summarizes recent advances bioprinted models, emphasizing their application studying cell‐ECM interactions, stromal‐tumor crosstalk, vasculature formation investigating occurrence metastasis, as well utility high‐throughput drug screening therapeutic development. Finally, discusses advantages challenges creating biomimetic while providing insights into future development trajectories potential translational applications research personalized medicine. By focusing these critical dimensions, aims to highlight transformative role advancing vitro models ultimate goal improving treatment, prevention, patient outcomes.

Language: Английский