Liquid–liquid phase transition as a basis for novel materials for skin repair and regeneration DOI Creative Commons
Shunfeng Wang, Meik Neufurth, Hadrian Schepler

et al.

Journal of Materials Chemistry B, Journal Year: 2024, Volume and Issue: 12(38), P. 9622 - 9638

Published: Jan. 1, 2024

Inorganic materials are of increasing interest not only for bone repair but also other applications in regenerative medicine. In this study, the combined effects energy-providing, regeneratively active inorganic polyphosphate (polyP) and morphogenetically pearl powder on wound healing were investigated. Aragonite, mineralic constituent nacre thermodynamically unstable form crystalline calcium carbonate, was found to be converted into a soluble state presence Ca2+-containing exudate, particularly upon addition sodium polyP (Na-polyP), driven by transfer Ca2+ ions from aragonite polyP, leading liquid-liquid phase separation an aqueous Ca-polyP coacervate. This process is further enhanced nanoparticles (Ca-polyP-NP). Kinetic studies revealed that coacervation exudate very rapid results formation stronger gel with porous structure compared alone. Coacervate formation, enabled transition Na-polyP/Ca-polyP-NP could demonstrated hydroxyethyl cellulose-based hydrogel used treatment. Furthermore, it shown together strongly enhances proliferation mesenchymal stem cells promotes microtube vitro angiogenesis assay HUVEC endothelial cells. The latter effect confirmed gene expression studies, applying real-time polymerase chain reaction, using biomarker genes VEGF (vascular growth factor) hypoxia-inducible factor-1 α (HIF-1α). Division Escherichia coli suppressed when suspended matrix containing aragonite. potential medical relevance these findings supported animal study genetically engineered diabetic mice (db/db), which marked increase granulation tissue microvessel regenerating experimental wounds treated Ca-polyP-NP controls. Co-administration significantly accelerated healing-promoting db/db mice. Based results, we propose ability mixed coacervate aragonite, its energy (ATP)-generating function, can decisively contribute activity polymer repair.

Language: Английский

Influence of Polyphosphate on the Mineralization Balance of Tooth Enamel DOI Creative Commons
Jing Ru, Xiaochen Xu, Yuxuan Cheng

et al.

ACS Omega, Journal Year: 2025, Volume and Issue: unknown

Published: March 4, 2025

Dental minerals are in an equilibrium state of demineralization and remineralization, which can be disrupted by pathogenic bacteria to cause dental caries. While the inorganic polymer polyphosphate (polyP) is ubiquitous living organisms also widely involved mineralization regulations, its specific influence on balance teeth remains unclear. As a concept-and-proof study, effects polyP remineralization investigated enamel (the highly mineralized outer covering tissue teeth) from perspective balance. We found that high concentration (containing 1.0-20 mM P element, comparable higher than free phosphate ions body fluids) has capability demineralize aqueous solution, yet this effect absent simulated biological environments including fluid MEM (α-minimum essential medium) solutions. More importantly, with very low ≥5.0 μM P) able inhibit significantly. This suggests could impact preferentially inhibiting process, thereby disrupting necessary for maintaining health.

Language: Английский

Citations

0
Molecular Mechanism of Polyphosphate-Mediated Nanosheet Self-Assembly DOI
Ming Yang, Shiyi Qin, Xuebo Quan

et al.

Langmuir, Journal Year: 2025, Volume and Issue: unknown

Published: May 15, 2025

Polyphosphate (polyP), a biocompatible and biodegradable polymer, holds significant promise for drug delivery applications. Recent studies reveal that polyP Mn2+ ions can self-assemble into nanosheets, with cetrimonium (CTA) acting as templating agent. However, the underlying molecular mechanism remains poorly understood. Using coarse-grained dynamics simulations, we CTA form stable, sandwich-like nanostructure, positioned at center. Self-assembly is driven by hydrophobic interactions, curvature controlled surface tension, which determined interplay of electrostatic interactions polyP/CTA interface. The addition oleate solution flattens structure, while higher polyP-to-CTA ratios promote penetration. These findings highlight power simulations in uncovering self-assembly mechanisms advancing applications delivery.

Language: Английский

Citations

0
Review: functionalization of biopolymer-based electrospun nanofibers for wound healing DOI Creative Commons
Basma Ekram

Journal of Materials Science, Journal Year: 2025, Volume and Issue: unknown

Published: May 22, 2025

Language: Английский

Citations

0
Energetic Calcium Phosphate Nanominerals for Osteoporosis Treatment DOI

Xitong Cheng,

Yizheng Li,

Xiaoxue Chui

et al.

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 9, 2024

Abstract Energy metabolism disorders leading to tissue destruction are major causes of osteoporosis. While efficacious, bone repair strategies that modulate energy pose considerable challenges. Herein, an energetic calcium phosphate nanominerals (ECPN) is developed using polyphosphate as source for osteoporosis treatment. ECPN promotes adenosine triphosphate (ATP) production in the physiological environment, providing attain metabolic homeostasis. It significantly enhances rBMSCs’ autophagy capacity by activating AMPK‐related pathway, promoting osteogenic differentiation, and rebuilding regeneration microenvironment. ECPN's unique nanostructure can fully mineralize collagen fibers, enhancing matrix's mechanical properties. In vivo, rapidly infiltrates osteoporotic bones, fills defects, mineralizes matrix, new‐bone formation. The repaired exhibits properties comparable those normal bones. balances time‐sensitive need immediate matrix mineralization long‐term construction microenvironment during potential this fuel generating functional nanomaterials engineering has been underestimated past. concept nanomineral may elicit new trends engineering.

Language: Английский

Citations

2
Liquid–liquid phase transition as a basis for novel materials for skin repair and regeneration DOI Creative Commons
Shunfeng Wang, Meik Neufurth, Hadrian Schepler

et al.

Journal of Materials Chemistry B, Journal Year: 2024, Volume and Issue: 12(38), P. 9622 - 9638

Published: Jan. 1, 2024

Inorganic materials are of increasing interest not only for bone repair but also other applications in regenerative medicine. In this study, the combined effects energy-providing, regeneratively active inorganic polyphosphate (polyP) and morphogenetically pearl powder on wound healing were investigated. Aragonite, mineralic constituent nacre thermodynamically unstable form crystalline calcium carbonate, was found to be converted into a soluble state presence Ca2+-containing exudate, particularly upon addition sodium polyP (Na-polyP), driven by transfer Ca2+ ions from aragonite polyP, leading liquid-liquid phase separation an aqueous Ca-polyP coacervate. This process is further enhanced nanoparticles (Ca-polyP-NP). Kinetic studies revealed that coacervation exudate very rapid results formation stronger gel with porous structure compared alone. Coacervate formation, enabled transition Na-polyP/Ca-polyP-NP could demonstrated hydroxyethyl cellulose-based hydrogel used treatment. Furthermore, it shown together strongly enhances proliferation mesenchymal stem cells promotes microtube vitro angiogenesis assay HUVEC endothelial cells. The latter effect confirmed gene expression studies, applying real-time polymerase chain reaction, using biomarker genes VEGF (vascular growth factor) hypoxia-inducible factor-1 α (HIF-1α). Division Escherichia coli suppressed when suspended matrix containing aragonite. potential medical relevance these findings supported animal study genetically engineered diabetic mice (db/db), which marked increase granulation tissue microvessel regenerating experimental wounds treated Ca-polyP-NP controls. Co-administration significantly accelerated healing-promoting db/db mice. Based results, we propose ability mixed coacervate aragonite, its energy (ATP)-generating function, can decisively contribute activity polymer repair.

Language: Английский

Citations

0