Phenylboronic Acid-Modified Membrane-Like Magnetic Quantum Dots Enable the Ultrasensitive and Broad-Spectrum Detection of Viruses by Lateral Flow Immunoassay

ACS Nano, Journal Year: 2024, Volume and Issue: 18(26), P. 16752 - 16765

Published: June 20, 2024

Although lateral flow immunochromatographic assay (LFIA) is an effective point-of-care testing technology, it still cannot achieve broad-spectrum and ultrasensitive detection of viruses. Herein, we propose a multiplex LFIA platform using two-dimensional graphene oxide (GO)-based magnetic fluorescent nanofilm (GF@DQD) as multifunctional probe 4-aminophenylboronic acid (APBA) recognition molecule for viral glycoprotein detection. GF@DQD-APBA with enhanced magnetic/fluorescence properties universal capture ability multiple viruses was easily prepared through the electrostatic adsorption one layer density-controlled Fe3O4 nanoparticles (NPs) thousands small CdSe/ZnS-MPA quantum dots (QDs) on monolayer GO sheet followed by chemical coupling APBA QD surface. The enabled specific determination different target test strip arbitrary combination antibody-modified strip, thus greatly improving efficiency reducing cost difficulty proposed technique can simultaneously sensitively diagnose three newly emerged within 20 min limits down to pg/mL level. excellent practicability GF@DQD-APBA-LFIA also demonstrated in 34 clinical specimens positive SARS-CoV-2, revealing its potential epidemic control on-site

Language: Английский

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Evaluation of the Multidimensional Enhanced Lateral Flow Immunoassay in Point-of-Care Nanosensors

ACS Nano, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 23, 2024

Point-of-care (POC) nanosensors with high screening efficiency show promise for user-friendly manipulation in the ever-increasing on-site analysis demand illness diagnosis, environmental monitoring, and food safety. Currently, inspired by merits of integrating advanced nanomaterials, molecular biology, machine learning, artificial intelligence, lateral flow immunoassay (LFIA)-based POC have been devoted to satisfying commercial demands terms sensitivity, specificity, practicality. Herein, we examine use multidimensional enhanced LFIA various fields over past two decades, focusing on introducing nanomaterials improve acquisition capability small order magnitude targets through engineering transformations emphasizing interdomain fusion collaboratively address inherent challenges current applications, such as multiplexing, development detectors quantitative analysis, more practical sensitivity enhancement. Specifically, this comprehensive review encompasses latest advances comprehending an alternative signal transduction pattern, aiming achieve rapid, ultrasensitive, "sample-to-answer" available options progressive applications nanosensors. In summary, cross-collaboration disciplines, has potential break barriers toward commercialization laboratory-level nanosensors, thus leading emergence next generation LFIA.

Language: Английский

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Advancements in magnetic nanomaterial-assisted sensitive detection of foodborne bacteria: Dual-recognition strategies, functionalities, and multiplexing applications

Food Chemistry, Journal Year: 2025, Volume and Issue: 478, P. 143626 - 143626

Published: Feb. 27, 2025

Language: Английский

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Integrating magnetic-plasmonic and membrane-like nanotags for the sensitive and reliable detection of aflatoxin B1 in foodstuffs

Food Control, Journal Year: 2025, Volume and Issue: unknown, P. 111144 - 111144

Published: Jan. 1, 2025

Language: Английский

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3D Film-Like Nanozyme with a Synergistic Amplification Effect for the Ultrasensitive Immunochromatographic Detection of Respiratory Viruses

ACS Nano, Journal Year: 2024, Volume and Issue: 18(37), P. 25865 - 25879

Published: Sept. 2, 2024

Greatly improving the sensitivity and detection range of lateral flow immunoassays (LFAs) by at least 100 times without using additional instruments remains challenging. Herein, we develop a three-dimensional (3D) film-like nanozyme (GO-Pt

Language: Английский

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Lateral flow assays: Progress and evolution of recent trends in point-of-care applications

Materials Today Bio, Journal Year: 2024, Volume and Issue: 28, P. 101188 - 101188

Published: Aug. 7, 2024

Paper based point-of-care (PoC) detection platforms applying lateral flow assays (LFAs) have gained paramount approval in the diagnostic domain as well environmental applications owing to their ease of utility, low cost, and rapid signal readout. It has centralized aspect self-evaluation exhibiting promising potential last global pandemic era Covid-19 implementing management public health remote areas. In this perspective, present review is focused towards landscaping current framework LFAs along with integration components characteristics for improving assay by pushing limits. The highlights synergistic aspects designing, sample enrichment strategies, novel nanomaterials-based transducers, high-end analytical techniques that contribute significantly sensitivity specificity enhancement. Various recent studies are discussed supporting innovations LFA systems focus upon accuracy reliability PoC testing. also provides a comprehensive overview all possible difficulties commercialization subjecting its applicability pathogen surveillance, water food testing, disease diagnostics, agriculture issues.

Language: Английский

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Fe3O4@Au magnetic SERS nanotags-based vertical flow immunoassay for simultaneous detection of fumonisin B1, aflatoxin B1 and deoxnivalenol

Analytica Chimica Acta, Journal Year: 2025, Volume and Issue: 1348, P. 343837 - 343837

Published: Feb. 21, 2025

Language: Английский

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Recent Advances in Surface-Enhanced Raman Scattering for Pathogenic Bacteria Detection: A Review

Sensors, Journal Year: 2025, Volume and Issue: 25(5), P. 1370 - 1370

Published: Feb. 23, 2025

Bacterial infection is one of the common infectious diseases in clinical practice, and research on efficient detection bacteria has attracted much attention recent years. Currently, traditional methods are mainly based cell culturing, microscopic examination, molecular biology techniques, all which have disadvantages complex operation time-consuming. Surface-enhanced Raman spectroscopy (SERS) technology shown prominent advantages bacterial identification because merit high-sensitivity, fast unique fingerprint spectrum. This paper investigates discusses application SERS detection, systematically reviews progress applications, including nano-enhanced dielectric materials SERS, signal amplification labeled molecules, integration with microfluidic technology. Finally, analyzes challenges associated offers insights into future development trends.

Language: Английский

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Silver Nanocubes/Nano Mica Platelets Flexible Nanohybrid Substrates Modified by Zinc Oxide Quantum Dots with Synergistic 3D Lightning Rod Effect, and Electromagnetic and Chemical Enhancements for Highly Sensitive SERS Bacterial Biosensor

Materials Today Nano, Journal Year: 2025, Volume and Issue: unknown, P. 100601 - 100601

Published: Feb. 1, 2025

Language: Английский

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Magnetically optimized surface enhanced Raman scattering detection strategy and its sensing applications

Coordination Chemistry Reviews, Journal Year: 2024, Volume and Issue: 510, P. 215848 - 215848

Published: April 11, 2024

Language: Английский

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