An Advanced Multivalent Ligand‐Decorated Microsphere Enrichment System Efficiently Captures Circulating Tumor Cells In Vivo

Small, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 7, 2025

Abstract Capturing circulating tumor cells (CTCs) in vivo from the bloodstream lessens metastasis and recurrence risks. However, absence of CTC receptors due to epithelial‐mesenchymal transition (EMT), limited binding capacity a single ligand, complexity blood flow environment significantly reduce efficiency capture vivo. Herein, multivalent ligand‐decorated microsphere enrichment system (MLMES) is crafted that incorporates column replete with an immunosorbent precisely recognizes binds stably expressed cluster differentiation 44 (CD44) glucose transporter protein 1 (GLUT1) present on exterior CTCs. As peripheral flows through column, CTCs are efficiently captured, achieving rate up 64.2%, highest reported date. Moreover, MLMES demonstrates excellent biocompatibility, broad‐spectrum capture, storage stability. Importantly, it eliminates substantial quantity blood, reducing risk metastasis. This breakthrough method has broad clinical application potential preventing recurrence, bringing new possibilities for improving cancer treatment.

Language: Английский

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A Cascaded Chip for the High-Purity Capture and Distinguishing Detection of Phenotypic Circulating Tumor Cells in Colon Cancer

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 11, 2025

The low abundance, complex phenotypes, and need for sophisticated blood preprocessing pose substantial obstacles to the clinical implementation of circulating tumor cells (CTCs). Herein, we constructed a cascaded PMMA chip-based platform separation CTCs from other within samples, as well distinguishing detection epithelial mesenchymal CTCs. primary physical chip (PS-Chip) focused sorted whole via Dean flow fractionation (DFF) according size differences between cells, being capable eliminating approximately 93.7% red (RBCs) 68.4% white (WBCs) while maintaining CTC recovery rate around 90%. Subsequently, further purify isolated in upstream, partitioned immunoaffinity capture (PICD-Chip) featuring with two independent chambers (Zone 1, Zone 2) was designed, each which premodified Gel-GO/E/V-Apt complexes that specifically recognize distinct enabling residual upstream isolation. Upon subsequent introduction probes, namely EpCAM vimentin aptamer-modified mesoporous Pt nanoparticles (mPtNPs/E/V-Apt), into 1 2, respectively, heterogeneous ranging 5 200/mL captured were distinguished quantified utilizing exceptional peroxidase activity mPtNPs. integrated approach efficient enrichment differentiation phenotypic under requirement high purity has enabled successful application diagnosis colon cancer patients at different stages.

Language: Английский

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Dual-mode photoelectrochemical/colorimetric sensor for CTCs detection based on seaurchin-like LaNiCo/Pd nanozymes and Exo-DNAzyme cascade program

Sensors and Actuators B Chemical, Journal Year: 2025, Volume and Issue: unknown, P. 137837 - 137837

Published: April 1, 2025

Language: Английский

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Distinct CTC Specific RNA Profile Enables NSCLC Early Detection and Dynamic Monitoring of Advanced NSCLC

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: April 3, 2025

Circulating tumor cells (CTCs) hold significant potential as biomarkers for the diagnosis and management of non-small cell lung cancer (NSCLC). However, their clinical utility is limited by heterogeneity CTC subtypes need robust, quantitative assays. In this study, a RNA assay incorporating multi-antibody-based isolation specific mRNA quantification RT-ddPCR developed. Two distinct models are established: NSCLC ScoreD detecting early (stages I-II), ScoreM monitoring advanced III-IV), based on cohort criteria. demonstrates high diagnostic performance early-stage NSCLC, achieving an area under receiver operating characteristic curve (AUC) 0.93, significantly outperforming serum CEA (AUC = 0.70). Compared to ScoreD, captures key gene feature KRT19, whose fragment protein, CYFRA 21-1, used prognostic biomarker NSCLC. Notably, ScoresM exhibits more accurate warning patient responses different therapies than CYFRA21-1 levels, which may provide blood test-based improved treatment assessment in

Language: Английский

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DNA network-enhanced electrochemical cytosensor for analyzing mesenchymal CTCs and epithelial-mesenchymal transition in ovarian cancer

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 162728 - 162728

Published: April 1, 2025

Language: Английский

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