Engineered CRISPR/Cas Ribonucleoproteins for Enhanced Biosensing and Bioimaging DOI

Linxin Cao,

Zeyuan Wang, Chunyang Lei

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 11, 2025

CRISPR-Cas systems represent a highly programmable and precise nucleic acid-targeting platform, which has been strategically engineered as versatile toolkit for biosensing bioimaging applications. Nevertheless, their analytical performance is constrained by inherent functional activity limitations of natural CRISPR/Cas systems, underscoring the critical role molecular engineering in enhancing capabilities. This review comprehensively examines recent advancements ribonucleoproteins (RNPs) to enhance capabilities advanced detection cellular imaging. We explore innovative strategies developing enhanced RNPs, including Cas protein through mutagenesis fusion techniques, guide RNA via chemical structural modifications. Furthermore, we evaluate these RNPs' applications sensitive biomarker live-cell genomic DNA monitoring, while analyzing current challenges prospective developments RNP bioimaging.

Language: Английский

Sea Urchin-like Magnetic Microbeads-Based Electrochemical Biosensor for Highly Sensitive Detection of Metabolites DOI Creative Commons

Bin Chen,

Xiaosu Yuan,

Enze Tian

et al.

Biosensors, Journal Year: 2025, Volume and Issue: 15(4), P. 225 - 225

Published: April 2, 2025

Analyzing metabolite levels in bodily fluids is essential for disease diagnosis and surveillance. Electrochemical biosensors are ideal monitoring due to their high sensitivity, rapid response, low cost. The magnetic microbeads-based electrode functionalization method further promotes the automation development of electrochemical by eliminating tedious polishing process. In this study, we presented sea urchin-like microbeads (SMMBs) constructed an SMMB-based biosensor. specific morphology SMMBs provides a larger surface area abundant enzyme binding sites, thereby expanding active reaction interface on improving sensitivity Experiment results demonstrated that biosensor achieves μM level detection glucose. Furthermore, replacing anchored oxidase SMMBs, can be extended detect other metabolites, such as cholesterol. summary, provide new path handily construct hold great potential development.

Language: Английский

Citations

0
Engineered CRISPR/Cas Ribonucleoproteins for Enhanced Biosensing and Bioimaging DOI

Linxin Cao,

Zeyuan Wang, Chunyang Lei

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 11, 2025

CRISPR-Cas systems represent a highly programmable and precise nucleic acid-targeting platform, which has been strategically engineered as versatile toolkit for biosensing bioimaging applications. Nevertheless, their analytical performance is constrained by inherent functional activity limitations of natural CRISPR/Cas systems, underscoring the critical role molecular engineering in enhancing capabilities. This review comprehensively examines recent advancements ribonucleoproteins (RNPs) to enhance capabilities advanced detection cellular imaging. We explore innovative strategies developing enhanced RNPs, including Cas protein through mutagenesis fusion techniques, guide RNA via chemical structural modifications. Furthermore, we evaluate these RNPs' applications sensitive biomarker live-cell genomic DNA monitoring, while analyzing current challenges prospective developments RNP bioimaging.

Language: Английский

Citations

0