Bioprinting of GelMA-Based Hydrogels to Aid in Creation of Biomimetic 3D Models for Glioblastoma DOI Creative Commons

Kaitlyn Ann Rose Schroyer,

Klaus‐Peter Schmitz,

Gunjeeta Raheja

et al.

Micromachines, Journal Year: 2025, Volume and Issue: 16(6), P. 654 - 654

Published: May 29, 2025

Glioblastoma (GBM, isocitrate dehydrogenase wild-type) is the most common primary malignant brain tumor in adults and associated with a severely low survival rate. Treatments offer mere palliation are ineffective, due, part, to lack of understanding intricate mechanisms underlying disease, including contribution microenvironment (TME). Current GBM models continue face challenges as they critical components properties required. To address this limitation, we developed innovative practical three-dimensional (3D) structural mechanical biomimicry tunability. These allowed for more accurate emulation extracellular matrix (ECM) vasculature characteristics native TME. Additionally, 3D bioprinting was utilized integrate these complexities, employing hydrogel composite mimic environment that known contribute cell growth. First, examined changes physical resulted from adjoining hydrogels at diverse concentrations using Fourier-Transform Infrared Spectroscopy (FTIR), compression testing, scanning electron microscopy (SEM), rheological analysis, degradation analysis. Subsequently, refined optimized embedded processes. The resulting were structurally reliable reproducible, featuring integrated inner channels possessing tunable emulate ECM. Biocompatibility testing performed via live/dead AlamarBlue analyses cells (both commercial lines patient-derived lines) encapsulated constructs, along immunohistochemistry staining understand how ECM altered functions cells. observed behavior indicated greater functionality softer matrices, while incorporation hyaluronic acid (HA) into gelatin methacryloyl (gelMA) enhanced its findings underscore role TME components, particularly properties, influencing survival, proliferation, molecular expression, laying groundwork further mechanistic studies. outcomes validate potential leveraging modeling, providing fully controllable explore specific pathways therapeutic targets challenging study conventional model systems.

Language: Английский

Exosomes in the Chemoresistance of Glioma: Key Point in Chemoresistance DOI Creative Commons

Xu Guo,

Haozhe Piao,

Rui Sui

et al.

Journal of Cellular and Molecular Medicine, Journal Year: 2025, Volume and Issue: 29(4)

Published: Feb. 1, 2025

ABSTRACT Gliomas are the most ordinary primary virulent brain tumours and commonly used clinical treatments include tumour resection, radiation therapy chemotherapy. Although significant progress has been made in recent years progression‐free survival (PFS) overall (OS) for patients with high‐grade gliomas, prognosis remains poor. Chemoresistance refers to phenomenon of decreased sensitivity cells drugs, resulting reduced or ineffective drug efficacy, is an important cause failure Exosomes, a type extracellular vesicle, secreted by cancer various stromal microenvironment (TME) transfer their inclusions cells, increasing chemoresistance. Furthermore, depletion exosomes reverses certain detrimental effects on metabolism restores chemotherapeutic agents. Here, we summarised correlation between resistance agents glioma patients, mechanisms action involved value. We aimed afford new thoughts research, diagnosis intervention chemoresistance patients.

Language: Английский

Citations

1
Unlocking the Human Blood-brain barrier (BBB) Characteristics for the Development of Nano-Delivery Strategies for Central Nervous System Therapies DOI
Cemile Gokce, Bağdagül Altun,

Esen Kirit

et al.

Journal of Drug Delivery Science and Technology, Journal Year: 2025, Volume and Issue: unknown, P. 106961 - 106961

Published: April 1, 2025

Language: Английский

Citations

0
Brain organoids-on-chip for neural diseases modeling: History, challenges and trends DOI Creative Commons
Hongyong Zhang, Nan Huang, Sumin Bian

et al.

Journal of Pharmaceutical Analysis, Journal Year: 2025, Volume and Issue: unknown, P. 101323 - 101323

Published: April 1, 2025

Language: Английский

Citations

0
Targeted eradication of glioblastoma via venom decapeptide-conjugated dendrimers: Inducing nuclear translocation and autophagic cell death DOI
Haixin Qin, Siyuan Luo, Weimin Zuo

et al.

Journal of Controlled Release, Journal Year: 2025, Volume and Issue: unknown, P. 113780 - 113780

Published: April 1, 2025

Language: Английский

Citations

0
Bioprinting of GelMA-Based Hydrogels to Aid in Creation of Biomimetic 3D Models for Glioblastoma DOI Creative Commons

Kaitlyn Ann Rose Schroyer,

Klaus‐Peter Schmitz,

Gunjeeta Raheja

et al.

Micromachines, Journal Year: 2025, Volume and Issue: 16(6), P. 654 - 654

Published: May 29, 2025

Glioblastoma (GBM, isocitrate dehydrogenase wild-type) is the most common primary malignant brain tumor in adults and associated with a severely low survival rate. Treatments offer mere palliation are ineffective, due, part, to lack of understanding intricate mechanisms underlying disease, including contribution microenvironment (TME). Current GBM models continue face challenges as they critical components properties required. To address this limitation, we developed innovative practical three-dimensional (3D) structural mechanical biomimicry tunability. These allowed for more accurate emulation extracellular matrix (ECM) vasculature characteristics native TME. Additionally, 3D bioprinting was utilized integrate these complexities, employing hydrogel composite mimic environment that known contribute cell growth. First, examined changes physical resulted from adjoining hydrogels at diverse concentrations using Fourier-Transform Infrared Spectroscopy (FTIR), compression testing, scanning electron microscopy (SEM), rheological analysis, degradation analysis. Subsequently, refined optimized embedded processes. The resulting were structurally reliable reproducible, featuring integrated inner channels possessing tunable emulate ECM. Biocompatibility testing performed via live/dead AlamarBlue analyses cells (both commercial lines patient-derived lines) encapsulated constructs, along immunohistochemistry staining understand how ECM altered functions cells. observed behavior indicated greater functionality softer matrices, while incorporation hyaluronic acid (HA) into gelatin methacryloyl (gelMA) enhanced its findings underscore role TME components, particularly properties, influencing survival, proliferation, molecular expression, laying groundwork further mechanistic studies. outcomes validate potential leveraging modeling, providing fully controllable explore specific pathways therapeutic targets challenging study conventional model systems.

Language: Английский

Citations

0