Intra-axonal Nanomagnetic Forces Differentially Impact hTau40 Transport Dynamics in Primary Cortical and Hippocampal Neurons DOI
Mackenna K. Landis, Anja Kunze

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 18, 2025

A crucial aspect of neural engineering is the ability to manipulate proteins that are substantially involved in axonal outgrowth and maintenance. Previous work this field has shown applying low-magnitude (piconewton) forces early stage neurons can result altered distributions critical structural proteins, such as microtubule-associated protein Tau. Uncovering mechanisms Tau redistribution could provide a tool for manipulating dysregulated forms protein. This study examined how transport responded intra-axonal nanomagnetic (NMFs) primary cortical hippocampal neurons. High magnification, live cell fluorescent imaging was employed visualize both full-length human (hTau40) amine-terminated, starch-coated magnetic nanoparticles (afMNPs) observe these cell-internal impact hTau40 within axon. Here, we found afMNPs acted by pulling on puncta under NMF application, especially cells, where were more likely be Forces greater than 1 pN enabled differentiated speeds displacement based relative force direction. data indicates utilized engineer transport, even cells at later stages maturation.

Language: Английский

Intra-axonal Nanomagnetic Forces Differentially Impact hTau40 Transport Dynamics in Primary Cortical and Hippocampal Neurons DOI
Mackenna K. Landis, Anja Kunze

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 18, 2025

A crucial aspect of neural engineering is the ability to manipulate proteins that are substantially involved in axonal outgrowth and maintenance. Previous work this field has shown applying low-magnitude (piconewton) forces early stage neurons can result altered distributions critical structural proteins, such as microtubule-associated protein Tau. Uncovering mechanisms Tau redistribution could provide a tool for manipulating dysregulated forms protein. This study examined how transport responded intra-axonal nanomagnetic (NMFs) primary cortical hippocampal neurons. High magnification, live cell fluorescent imaging was employed visualize both full-length human (hTau40) amine-terminated, starch-coated magnetic nanoparticles (afMNPs) observe these cell-internal impact hTau40 within axon. Here, we found afMNPs acted by pulling on puncta under NMF application, especially cells, where were more likely be Forces greater than 1 pN enabled differentiated speeds displacement based relative force direction. data indicates utilized engineer transport, even cells at later stages maturation.

Language: Английский

Citations

0