UV-Cross-Linked DNA Nanomaterials Enable Robust Nanopatterning of Folate Ligands for Enhanced Cellular Uptake DOI
Tyler Brown,

Daniel Saliba,

Jakob Hartmann

et al.

Journal of the American Chemical Society, Journal Year: 2025, Volume and Issue: unknown

Published: May 22, 2025

The arrangement of ligands on a nanomaterial scaffold is powerful approach to enhance targeted cellular delivery. However, nanomaterial-mediated delivery often employs imprecise ligand conjugation, limiting the exploration optimal density and spatial organization. To address these challenges, we developed DNA nanomaterials with precisely spaced folate rigidified them via postassembly UV-based thymine cross-linking. These materials exhibit exceptional nuclease stability maintain structural integrity both under biologically relevant conditions during internalization into live HeLa cells. We used as scaffolds for patterning identified modes presentation uptake. Each step uptake process was probed, revealing synergistic effects stabilization precise mechanism, intracellular retention, export dynamics. then our nanopatterned vectors gene-silencing nucleic acid payload. By integrating optimized immobilization, successfully achieved gene silencing in receptor alpha-expressing cancer This work showcases effect that nanostructure fidelity rigidity have moieties. It introduces UV cross-linking critical tool nanomaterials, enabling applications therapeutic delivery, diagnostics, nanoscale cell surface engineering. In addition, this study reveals principles nanopatterning future systems precision, stability, biocompatibility.

Language: Английский

UV-Cross-Linked DNA Nanomaterials Enable Robust Nanopatterning of Folate Ligands for Enhanced Cellular Uptake DOI
Tyler Brown,

Daniel Saliba,

Jakob Hartmann

et al.

Journal of the American Chemical Society, Journal Year: 2025, Volume and Issue: unknown

Published: May 22, 2025

The arrangement of ligands on a nanomaterial scaffold is powerful approach to enhance targeted cellular delivery. However, nanomaterial-mediated delivery often employs imprecise ligand conjugation, limiting the exploration optimal density and spatial organization. To address these challenges, we developed DNA nanomaterials with precisely spaced folate rigidified them via postassembly UV-based thymine cross-linking. These materials exhibit exceptional nuclease stability maintain structural integrity both under biologically relevant conditions during internalization into live HeLa cells. We used as scaffolds for patterning identified modes presentation uptake. Each step uptake process was probed, revealing synergistic effects stabilization precise mechanism, intracellular retention, export dynamics. then our nanopatterned vectors gene-silencing nucleic acid payload. By integrating optimized immobilization, successfully achieved gene silencing in receptor alpha-expressing cancer This work showcases effect that nanostructure fidelity rigidity have moieties. It introduces UV cross-linking critical tool nanomaterials, enabling applications therapeutic delivery, diagnostics, nanoscale cell surface engineering. In addition, this study reveals principles nanopatterning future systems precision, stability, biocompatibility.

Language: Английский

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