Effect of Tyrosine-Containing Self-Assembling β-Sheet Peptides on Macrophage Polarization and Inflammatory Response DOI Creative Commons
Jacek K. Wychowaniec, Ezgi Irem Bektas,

Marcia Muerner

et al.

ACS Applied Materials & Interfaces, Journal Year: 2025, Volume and Issue: unknown

Published: April 15, 2025

Self-assembling peptides (SAPs) are fully defined nanobiomaterials offering unprecedented opportunities to control nanostructure and chemical attributes investigate manipulate cellular signals. To the influence of morphological characteristics on inflammatory signaling in native immunity, we designed five β-sheet SAPs: EFEFKFEFK (EF8), YEFEFKFEFK (YEF8), EFEFKFEFKY (EF8Y), YEFEFKFEFKY (YEF8Y), EYEFKFEFK (EYF8) (F: phenylalanine; E: glutamic acid; K: lysine, Y: tyrosine). The position tyrosine peptide sequence dictated self-assembly into nanostructures, with all SAPs self-assembling thin constituent nanofibers d ≈ 3.8 ± 0.4 nm, sequences YEF8 EF8 showing a propensity for associative bundling. These distinct induced contrasting responses monocytic model THP-1 cells-derived macrophages (MΦs). Presence soluble (at 2 mM) an anti-inflammatory response polarization toward M2 state, whereas displayed tendency inducing pro-inflammatory M1 state. EF8Y, YEF8Y, EYF8 did not induce our models. results were validated using peripheral blood mononuclear cells (PBMCs)-derived MΦs from human donors, confirming critical role as possible orchestrators repair tissues or inducers respectively. same THP-1-derived cultured 20 mM hydrogels obtained. findings will facilitate utilization this family immunomodulatory potentially changing course inflammation during progression various diseases.

Language: Английский

Noninvasive Monitoring of Palmitoyl Hexapeptide-12 in Human Skin Layers: Mechanical Interaction with Skin Components and Its Potential Skincare Benefits DOI Creative Commons
Cosimo Ligorio,

Elham Tavasoli,

Nevena Karaman‐Jurukovska

et al.

ACS Applied Bio Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 18, 2025

Self-assembling peptides (SAPs) represent a rich source of building blocks that interact with biological structures. For instance, cosmetic SAPs like Palmitoyl hexapeptide-12 have gained increasing interest for their anti-aging properties. However, short-term impact on the skin composition and mechanics remains unclear. In this study, battery label-free techniques is exploited to objectively monitor effects human skin. Orbital trapping secondary ion mass spectrometry (OrbiSIMS) used discern between sol gel forms, tracking its self-assembly penetration within full-thickness shown permeate both stratum corneum epidermal layers, initiating formation by harnessing endogenous ions. Hence, ability peptide strengthen repair barrier after delipidation also demonstrated through high-throughput mechanical characterization stimulated Raman scattering (SRS). Finally, co-assembling properties native molecules are via in vitro tests ex vivo histology. This study establishes methodological benchmark measuring hydration, introducing platform design capable create "biocooperative" structures benefits.

Language: Английский

Citations

0
Effect of Tyrosine-Containing Self-Assembling β-Sheet Peptides on Macrophage Polarization and Inflammatory Response DOI Creative Commons
Jacek K. Wychowaniec, Ezgi Irem Bektas,

Marcia Muerner

et al.

ACS Applied Materials & Interfaces, Journal Year: 2025, Volume and Issue: unknown

Published: April 15, 2025

Self-assembling peptides (SAPs) are fully defined nanobiomaterials offering unprecedented opportunities to control nanostructure and chemical attributes investigate manipulate cellular signals. To the influence of morphological characteristics on inflammatory signaling in native immunity, we designed five β-sheet SAPs: EFEFKFEFK (EF8), YEFEFKFEFK (YEF8), EFEFKFEFKY (EF8Y), YEFEFKFEFKY (YEF8Y), EYEFKFEFK (EYF8) (F: phenylalanine; E: glutamic acid; K: lysine, Y: tyrosine). The position tyrosine peptide sequence dictated self-assembly into nanostructures, with all SAPs self-assembling thin constituent nanofibers d ≈ 3.8 ± 0.4 nm, sequences YEF8 EF8 showing a propensity for associative bundling. These distinct induced contrasting responses monocytic model THP-1 cells-derived macrophages (MΦs). Presence soluble (at 2 mM) an anti-inflammatory response polarization toward M2 state, whereas displayed tendency inducing pro-inflammatory M1 state. EF8Y, YEF8Y, EYF8 did not induce our models. results were validated using peripheral blood mononuclear cells (PBMCs)-derived MΦs from human donors, confirming critical role as possible orchestrators repair tissues or inducers respectively. same THP-1-derived cultured 20 mM hydrogels obtained. findings will facilitate utilization this family immunomodulatory potentially changing course inflammation during progression various diseases.

Language: Английский

Citations

0