Liquid Biopsy on Microfluidics: From Existing Endogenous to Emerging Exogenous Biomarkers Analysis DOI

Xiaowen Ou,

Peng Chen, Bi‐Feng Liu

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 18, 2025

Liquid biopsy is an appealing approach for early diagnosis and assessment of treatment efficacy in cancer. Typically, liquid involves the detection endogenous biomarkers, including circulating tumor cells (CTCs), extracellular vesicles (EVs), DNA (ctDNA), RNA (ctRNA), proteins. The levels these biomarkers are higher cancer patients compared to those healthy individuals. However, clinical application using biomarker analysis faces challenges due its low abundance poor stability circulation. Recently, a promising strategy involving engineering exogenous probes has been developed overcome limitations. These activated within microenvironment, generating distinct markers that can be easily distinguished from background biological signals. Alternatively, labeled with intrinsic vivo detected vitro after metabolic processes. In this review, we primarily focus on microfluidic-based techniques allow transition analyzing existing emerging ones. First, introduce common as well synthetic Next, discuss recent advancements both biomarkers. Lastly, provide insights into future directions microfluidic systems.

Language: Английский

Fully Integrated Centrifugal Microfluidics for Rapid Exosome Isolation, Glycan Analysis, and Point-of-Care Diagnosis DOI
Xudong Zhao, Xiang Liu, Tucan Chen

et al.

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 27, 2025

Exosomes present in the circulatory system demonstrate considerable promise for diagnosis and treatment of diseases. Nevertheless, complex nature blood samples prevalence highly abundant proteins pose a significant obstacle to prompt effective isolation functional evaluation exosomes from blood. Here, we fully integrated lab-on-a-disc equipped with two nanofilters, also termed iExoDisc, which facilitates automated 400 μL within 45 min. By integrating plasma separation module, protein removal nanopore membrane-based total resulting exhibited significantly increased purity (∼3-6-fold) compared conventional ultracentrifugation polymer precipitation. Additionally, then successfully performed nontargeted targeted glycan profiling on derived clinical triple-negative breast cancer (TNBC) patients using MALDI-TOF-MS lectin microarray containing 56 kinds lectins. The findings both methodologies indicated that galactosylation sialylation exhibit potential as diagnostic indicators TNBC. Finally, by utilizing exosome-specific glycosylated CD63 proof-of-concept, realized integration point-of-care on-chip exosome situ detection 2 h. Thus, iExoDisc provides approach early detection, liquid biopsy, diagnosis.

Language: Английский

Citations

1
Artificial Intelligence Enhanced Microfluidic System for Multiplexed Point-of-care-testing of Biological Thiols DOI
Siyu Gao, Jingjing Wang,

Zeyu Miao

et al.

Talanta, Journal Year: 2025, Volume and Issue: 287, P. 127619 - 127619

Published: Jan. 23, 2025

Language: Английский

Citations

0
Freeze–Thaw Imaging for Microorganism Classification Assisted with Artificial Intelligence DOI
Han Xie,

Zhu Xubin,

Kaiyu Chen

et al.

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 19, 2025

Fast and cost-effective microbial classification is crucial for clinical diagnosis, environmental monitoring, food safety. However, traditional methods encounter challenges including intricate procedures, skilled personnel needs, sophisticated instrumentations. Here, we propose a microbe system, also termed freeze-thaw-induced floating pattern of AuNPs (FTFPA), coupled with artificial intelligence, which capable identifying microbes at cost $0.0023 per sample. Specifically, FTFPA utilizes coincubation microbes, resulting in distinct patterns upon freeze-thawing due to their weak interaction. These are digitized train models that distinguish nine various tasks. The positive sample detection model achieved an F1 score 0.976 (n = 194), while the multispecies task reached macro 0.859 1728). To address scalability lightweight requirements across diverse scenarios, categorized based on species levels. hierarchical 5184), order level Enterobacteriales 2550), Bacillales 1974) was 0.854, 0.907, 0.958, 0.843. In summary, our method user-friendly, requiring only simple equipment, easy operate, convenient, providing platform identification.

Language: Английский

Citations

0
Liquid Biopsy on Microfluidics: From Existing Endogenous to Emerging Exogenous Biomarkers Analysis DOI

Xiaowen Ou,

Peng Chen, Bi‐Feng Liu

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 18, 2025

Liquid biopsy is an appealing approach for early diagnosis and assessment of treatment efficacy in cancer. Typically, liquid involves the detection endogenous biomarkers, including circulating tumor cells (CTCs), extracellular vesicles (EVs), DNA (ctDNA), RNA (ctRNA), proteins. The levels these biomarkers are higher cancer patients compared to those healthy individuals. However, clinical application using biomarker analysis faces challenges due its low abundance poor stability circulation. Recently, a promising strategy involving engineering exogenous probes has been developed overcome limitations. These activated within microenvironment, generating distinct markers that can be easily distinguished from background biological signals. Alternatively, labeled with intrinsic vivo detected vitro after metabolic processes. In this review, we primarily focus on microfluidic-based techniques allow transition analyzing existing emerging ones. First, introduce common as well synthetic Next, discuss recent advancements both biomarkers. Lastly, provide insights into future directions microfluidic systems.

Language: Английский

Citations

0