The Analyst, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 1, 2025
A chemiluminescence enzyme immunoassay based on microfluidic magnetic droplets for multiplex sepsis biomarker screening, which has great potential broader applications in biomolecule detection and early disease diagnosis, is reported.
Language: Английский
Advanced Science, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 28, 2025
Navigating complex extracellular environments requires extensive deformation of cells and their nuclei. Most in vitro systems used to study nuclear deformations impose whole-cell confinement that mimics the physical crowding experienced by during 3D migration through tissues. Such systems, however, do not reproduce types expected occur line tissues such as endothelial or epithelial whose stems principally from topography underlying basement membrane. Here, it is shown myoblasts cultured on microgroove substrates mimic anisotropic membrane exhibit large-scale deformations, with partial complete penetration into microgrooves. These lead significant DNA damage are dynamic nuclei cyclically entering exiting Atomic force microscopy measurements show these cycles accompanied transient changes perinuclear stiffness. Interestingly, grooves driven cell-substrate adhesion stresses, a limited need for cytoskeleton-associated forces. Finally, demonstrated laminopathy patients abnormal microgrooves, raising possibility using novel functional diagnostic platform pathologies involve mechanics.
Language: Английский
Citations
0
Biomicrofluidics, Journal Year: 2025, Volume and Issue: 19(1)
Published: Jan. 1, 2025
Atmospheric ice-nucleating particles (INPs) make up a vanishingly small proportion of atmospheric aerosol but are key to triggering the freezing supercooled liquid water droplets, altering lifetime and radiative properties clouds having substantial impact on weather climate. However, INPs notoriously difficult model due lack information their global sources, sinks, concentrations, activity, necessitating development new instrumentation for quantifying characterizing in rapid automated manner. Microfluidic technology has been increasingly adopted by ice nucleation research groups recent years as means performing droplet analysis INPs, enabling measurement hundreds or thousands droplets per experiment at temperatures down homogeneous water. The potential microfluidics extends far beyond this, with an entire toolbox bioanalytical separation detection techniques developed over 30 medical applications. Such methods could easily be adapted biological biogenic INP revolutionize field, example, identification quantification bacteria fungi. Combined miniaturized sampling techniques, we can envisage deployment microfluidic sample-to-answer platforms automated, user-friendly field that would enable greater understanding seasonal activity. Here, review various components such platform incorporate highlight feasibility, challenges, endeavor, from assays separations bioanalysis.
Language: Английский
Citations
0
Electrophoresis, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 26, 2025
ABSTRACT We developed a novel electrorotation (ROT) device featuring microwell array with three electrodes. This allows to monitor the increase in membrane capacitance of cells subjected chemical stimulation. The is integrated into bottom fluidic channel and holds rotating during stimulation solution containing agent. Positive dielectrophoresis (p‐DEP) effectively traps microwells, whereas negative DEP (n‐DEP) facilitates rapid formation single‐cell presence. Alternating current (AC) voltages 120° phase shift applied across electrodes enable vertical simultaneous rotation cells. observed peak rate as function frequency, frequency spectrum shifting lower frequencies increased. A positive correlation was identified between capacitance, so monitoring low‐frequency range advantageous. Although n‐DEP at risks removing from continuous ROT achieved by regulating height center demonstrated induced Ca 2+ influx ionomycin. simple configuration statistical analysis rates without fluorescent labeling, making it suitable for label‐free assessments white blood cells’ responses stimuli.
Language: Английский
Citations
0
Analytica Chimica Acta, Journal Year: 2025, Volume and Issue: 1355, P. 343894 - 343894
Published: March 4, 2025
Shear flow deformability cytometry is an emerging microfluidic technique that has undergone significant advances in the last few years and offers considerable potential for clinical diagnostics disease monitoring. By simultaneously measuring mechanical morphological parameters of single cells, it a comprehensive extension traditional cell analysis, delivering unique insight into deformability, which gaining recognition as novel biomarker health disease. Due to its operating principle, method particularly suitable analysis blood samples. This review focuses on recent developments shear cytometry, widely adopted variant cytometry. It strong applications practice due robust simple operation, demonstrated with whole samples, well high throughput, can reach approximately 1000 cells per second. We begin by discussing some basic factors influence properties give overview operational principles samples from blood, cultured tissues. Next, we clinically relevant cancer cells. Finally, address key challenges adoption, such regulatory approval, scalable manufacturing, workflow integration, emphasizing need further validation studies facilitate implementation. article uniquely emphasizes relevance giving biomarkers studied In addition, addresses critical barriers translation. identifying these obstacles, this aims demonstrate bridge gap between research routine medical practice.
Language: Английский
Citations
0
The Analyst, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 1, 2025
A chemiluminescence enzyme immunoassay based on microfluidic magnetic droplets for multiplex sepsis biomarker screening, which has great potential broader applications in biomolecule detection and early disease diagnosis, is reported.
Language: Английский
Citations
0