Screening Oxidoreductase Activities of Nanozymes: Toward Activity‐Tailored Guidelines DOI Creative Commons
Giulia Mirra, Lorenzo Cursi, Luca Boselli

et al.

Small Science, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 6, 2024

Nanozymes (NZs) are nanomaterials able to mimic natural enzymes, offering the advantages of better durability, wider range operational conditions, and easier functionalization. In addition, some NZs exhibit multiple enzyme‐like activities effective tandem/self‐cascade reactions. this context, metallic metal–oxide nanoparticles among most promising candidates for biomedical/biotechnological applications, thanks their efficient oxidoreductase activities. However, characterizing is challenging because experimental set‐up different assays strongly influence performances. Consequently, currently available literature provides limited understanding characteristics various NZs, especially in terms activity‐related performance, hindering optimal selection applicative potential. Here, leveraging on accurate characterization methodology, we report a direct comparison several (gold‐Au, platinum‐Pt, palladium‐Pd, ceria, iron oxide) possessing multi‐enzymatic Our main findings indicate that 1) PtNZs outperform other tested catalase‐like activity; 2) Pt PdNZs present superior superoxide dismutase‐like 3) peroxidase‐ oxidase‐like activity, best‐performing NZ substrate‐dependent; 4) competitive reactions multifunctional play key role specific AuNZs can be choice peroxidase/oxidase configurations; 5) more than metal‐oxide ones, but 6) ceria presents unique phosphatase‐like activity.

Language: Английский

Screening Oxidoreductase Activities of Nanozymes: Toward Activity‐Tailored Guidelines DOI Creative Commons
Giulia Mirra, Lorenzo Cursi, Luca Boselli

et al.

Small Science, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 6, 2024

Nanozymes (NZs) are nanomaterials able to mimic natural enzymes, offering the advantages of better durability, wider range operational conditions, and easier functionalization. In addition, some NZs exhibit multiple enzyme‐like activities effective tandem/self‐cascade reactions. this context, metallic metal–oxide nanoparticles among most promising candidates for biomedical/biotechnological applications, thanks their efficient oxidoreductase activities. However, characterizing is challenging because experimental set‐up different assays strongly influence performances. Consequently, currently available literature provides limited understanding characteristics various NZs, especially in terms activity‐related performance, hindering optimal selection applicative potential. Here, leveraging on accurate characterization methodology, we report a direct comparison several (gold‐Au, platinum‐Pt, palladium‐Pd, ceria, iron oxide) possessing multi‐enzymatic Our main findings indicate that 1) PtNZs outperform other tested catalase‐like activity; 2) Pt PdNZs present superior superoxide dismutase‐like 3) peroxidase‐ oxidase‐like activity, best‐performing NZ substrate‐dependent; 4) competitive reactions multifunctional play key role specific AuNZs can be choice peroxidase/oxidase configurations; 5) more than metal‐oxide ones, but 6) ceria presents unique phosphatase‐like activity.

Language: Английский

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