Light‐Triggered Protease‐Mediated Release of Actin‐Bound Cargo from Synthetic Cells DOI Creative Commons
Mousumi Akter, Hossein Moghimianavval, Gary D. Luker

et al.

Advanced Biology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 18, 2025

Abstract Synthetic cells offer a versatile platform for addressing biomedical and environmental challenges, due to their modular design capability mimic cellular processes such as biosensing, intercellular communication, metabolism. Constructing synthetic capable of stimuli‐responsive secretion is vital applications in targeted drug delivery biosensor development. Previous attempts at engineering have been confined non‐specific cargo release via membrane pores, limiting the spatiotemporal precision specificity necessary selective secretion. Here, protein‐based termed TEV Protease‐mediated Releasable Actin‐binding Protein (TRAP) designed constructed selective, rapid, triggerable cells. TRAP bind tightly reconstituted actin networks proteolytically released from bound actin, followed by cell‐penetrating peptide translocation. TRAP's efficacy facilitating light‐activated both fluorescent luminescent proteins demonstrated. By equipping with controlled mechanism, paves way development biomaterials, cell‐based biosensing systems, therapeutic through integration living protein therapeutics.

Language: Английский

Light‐Triggered Protease‐Mediated Release of Actin‐Bound Cargo from Synthetic Cells DOI Creative Commons
Mousumi Akter, Hossein Moghimianavval, Gary D. Luker

et al.

Advanced Biology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 18, 2025

Abstract Synthetic cells offer a versatile platform for addressing biomedical and environmental challenges, due to their modular design capability mimic cellular processes such as biosensing, intercellular communication, metabolism. Constructing synthetic capable of stimuli‐responsive secretion is vital applications in targeted drug delivery biosensor development. Previous attempts at engineering have been confined non‐specific cargo release via membrane pores, limiting the spatiotemporal precision specificity necessary selective secretion. Here, protein‐based termed TEV Protease‐mediated Releasable Actin‐binding Protein (TRAP) designed constructed selective, rapid, triggerable cells. TRAP bind tightly reconstituted actin networks proteolytically released from bound actin, followed by cell‐penetrating peptide translocation. TRAP's efficacy facilitating light‐activated both fluorescent luminescent proteins demonstrated. By equipping with controlled mechanism, paves way development biomaterials, cell‐based biosensing systems, therapeutic through integration living protein therapeutics.

Language: Английский

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