A Miniature Modular Fluorescence Flow Cytometry System DOI Creative Commons
Shaoqi Huang, Jiale Li, Wei Li

et al.

Biosensors, Journal Year: 2024, Volume and Issue: 14(8), P. 395 - 395

Published: Aug. 16, 2024

Fluorescence flow cytometry is a powerful instrument to distinguish cells or particles labelled with high-specificity fluorophores. However, traditional complex, bulky, and inconvenient for users adjust fluorescence channels. In this paper, we present modular (M-FCM) system in which channels can be flexibly arranged. Modules particle focusing detection were developed. After hydrodynamical focusing, the measured modules, integrated situ illumination detection. The signal-to-noise ratio of reached 33.2 dB. crosstalk among was eliminated. M-FCM applied evaluate cell viability drug screening, agreeing well commercial cytometry. presents several outstanding features: flexibility setting channels, cost efficiency, compact construction, ease operation, potential upgrade multifunctional measurements. provides platform various biophysical measurements, e.g., electrical impedance refractive-index proposed work paves an innovative avenue multivariate analysis cellular characteristics.

Language: Английский

Multi-Vortex Regulation in a Simple Semicircular Microchannel with Ordered Micro-Obstacles for High-Throughput Buffer Exchange DOI
Shaofei Shen, Furong Zhang, Haodong Li

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 20, 2025

Microfluidics is an emerging technology for buffer exchange in bioprocessing applications. However, achieving with simplicity of operation and high throughput a straightforward channel design remains challenge. This study presents novel semicircular microchannel that allows the deterministic regulation helical Dean vortices through geometric confinement. By incorporating micro-obstacles into microchannels large dimensions (900 μm wide 100 high), we observe substantial enhancement secondary flows, leading to unique fluid distribution across range flow rates. enables particle separation efficiency (>96.27%) coupled low fluorescein purity (<4.46%) at 3 × 106 particles/min. The proposed methodology, characterized by ease production (simple dimensions), user-friendly (uniform rates both sheath sample inlets), efficient capabilities (typically mL min–1), demonstrates significant potential advancing microfluidic systems biological biomedical research.

Language: Английский

Citations

0
Biophysical assays to test cellular mechanosensing: moving towards high throughput DOI Creative Commons

Marta Cubero-Sarabia,

Anna Maria Kapetanaki,

Massimo Vassalli

et al.

Biophysical Reviews, Journal Year: 2024, Volume and Issue: 16(6), P. 875 - 882

Published: Dec. 1, 2024

Mechanosensitivity is the ability of cells to sense and respond mechanical stimuli. In order do this, are endowed with different components that allow them react a broad range stimuli, such as compression or shear forces, pressure, vibrations. This sensing process, mechanosensing, involved in fundamental physiological mechanisms, stem cell differentiation migration, but it also central development pathogenic states. Here, we review approaches have been proposed quantify mechanosensation living cells, specific focus on methodologies enable higher experimental throughput. aspect crucial fully understand nuances how impacts physiology pathology systems. We will discuss traditional methods for studying mechanosensing at level single particular attention activation mechanosensitive ion channel piezo1. Moreover, present recent attempts push analysis towards

Language: Английский

Citations

2
Research on the Method of Detecting TPN-Labeled Tumor Cells in Pleural Effusion Based on the Microfluidic Chip DOI Creative Commons

Xiaoyi Xun,

Shuang Song,

Yiran Luan

et al.

Micromachines, Journal Year: 2024, Volume and Issue: 15(8), P. 981 - 981

Published: July 30, 2024

The clinical diagnosis of a malignant pleural effusion (MPE) is still based on the detection tumor cells in effusion. question how to improve efficiency and accuracy detecting an MPE remains. This study explores use microfluidic technology concentrate achieved cell marker TPN microarray capture area. mitochondria-specific bio-probe that can identify basis differences mitochondrial potential. First, we designed chip analyze its performance. results show when total flow rate injected was 12 mL/h volume ratio separation liquid suspension 1:1, target (A549, MCF-7, Hela) were enriched purity improved 98.7-99.3%. Finally, from cancer patients used detect chip's ability isolate enrich cells. Furthermore, fluorescent identification within simultaneously chip. In conclusion, potential MPEs provided by structure analysis conditions explored this study.

Language: Английский

Citations

0
Continuous Inertial Alignment and Isolation of Spherical Microparticles and Nonspherical Flagellate Microalgae DOI
Xiaoming Chen,

Chungang Wu,

Jishun Shi

et al.

Journal of Chromatography A, Journal Year: 2024, Volume and Issue: 1739, P. 465509 - 465509

Published: Nov. 9, 2024

Language: Английский

Citations

0
A Microfluidic-Based Sensing Platform for Rapid Quality Control on Target Cells from Bioreactors DOI Creative Commons
Alessia Foscarini, Fabio Romano, Valeria Garzarelli

et al.

Sensors, Journal Year: 2024, Volume and Issue: 24(22), P. 7329 - 7329

Published: Nov. 16, 2024

We investigated the design and characterization of a Lab-On-a-Chip (LoC) cell detection system primarily designed to support immunotherapy in cancer treatment. Immunotherapy uses Chimeric Antigen Receptors (CARs) T Cell (TCRs) fight cancer, engineering response immune system. In recent years, it has emerged as promising strategy for personalized However, requires bioreactor-based culture expansion manual quality control (QC) modified cells, which is time-consuming, labour-intensive, prone errors. The miniaturized LoC device automated QC demonstrated here simple, low cost, reliable. Its final target become one building blocks an immunotherapy, would take place present labs procedures benefit throughput affordability. core commercial, on-chip-integrated capacitive sensor managed by microcontroller capable sensing cells accurately measured charge variations. hardware based on standardized components, makes suitable mass manufacturing. Moreover, unlike other solutions, no external AC source required. been characterized with line model selectively labelled gold nanoparticles simulate its future use bioreactors labelling can apply successfully engineered CAR-T-cells. Experiments were run both air-free drop microfluidics-and channel, where fluid volume was considerably lower than drop. showed good sensitivity even number cells-around 120, compared 10

Language: Английский

Citations

0
Cell elasticity measurement and sorting based on microfluidic techniques: advances and applications DOI Creative Commons

Jiahuan Yang,

Yong Liu, Bin Li

et al.

Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: 271, P. 116985 - 116985

Published: Nov. 25, 2024

Language: Английский

Citations

0
A Miniature Modular Fluorescence Flow Cytometry System DOI Creative Commons
Shaoqi Huang, Jiale Li, Wei Li

et al.

Biosensors, Journal Year: 2024, Volume and Issue: 14(8), P. 395 - 395

Published: Aug. 16, 2024

Fluorescence flow cytometry is a powerful instrument to distinguish cells or particles labelled with high-specificity fluorophores. However, traditional complex, bulky, and inconvenient for users adjust fluorescence channels. In this paper, we present modular (M-FCM) system in which channels can be flexibly arranged. Modules particle focusing detection were developed. After hydrodynamical focusing, the measured modules, integrated situ illumination detection. The signal-to-noise ratio of reached 33.2 dB. crosstalk among was eliminated. M-FCM applied evaluate cell viability drug screening, agreeing well commercial cytometry. presents several outstanding features: flexibility setting channels, cost efficiency, compact construction, ease operation, potential upgrade multifunctional measurements. provides platform various biophysical measurements, e.g., electrical impedance refractive-index proposed work paves an innovative avenue multivariate analysis cellular characteristics.

Language: Английский

Citations

0