Detection of protein oligomers with nanopores

Nature Reviews Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 5, 2025

Language: Английский

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Investigating the impact of Cu2+ on α-synuclein aggregation: A single-molecule approach

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 142161 - 142161

Published: March 1, 2025

Language: Английский

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Unveiling the Assembly Transition of Diphenylalanine and Its Analogs: from Oligomer Equilibrium to Nanocluster Formation

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: March 26, 2025

Peptide self-assembly is fundamental to various biological processes and holds significant potential for nanotechnology biomedical applications. Despite progress in understanding larger-scale assemblies, the early formation of low-molecular-weight oligomers remains poorly understood. In this study, we investigate aggregation behavior self-assembling diphenylalanine (FF) peptide its analogs. Utilizing single-nanopore analysis, detected characterized low-molecular-oligomer FF, N-tert-butoxycarbonyl-diphenylalanine (BocFF), fluorenylmethyloxycarbonyl-diphenylalanine (FmocFF), fluorenylmethyloxycarbonyl-pentafluoro-phenylalanine (Fmoc-F5-Phe) real time. This approach provided detailed insights into stages self-assembly, revealing dynamic kinetics oligomeric species. Analysis revealed that trimer key nucleus while dimer primary FmocFF Fmoc-F5-Phe aggregation, whereas both serve as nuclei BocFF. Mass photometry was employed track evolution these oligomers, transition from low- high-molecular-weight species, thereby elucidating intermediate phases process. Transmission electron microscopy Fourier transform infrared spectroscopy were further characterize final assembly states, offering high-resolution imaging morphological structures information on secondary structures. Based analyses, constructed a comprehensive graph correlates entire tested peptides across multiple scales. integrative provides holistic particularly toward mature supramolecular These findings shed light their pathways structural properties, advancing our

Language: Английский

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Multidimensional Investigations of Single Molecule Unfolding of Bovine Serum Albumin Using Plasmonic Nanopores

Nano Letters, Journal Year: 2025, Volume and Issue: unknown

Published: April 3, 2025

Direct detection of proteins, especially their conformation and configuration information, at the single molecule level, is challenging in various biotechnological fields. Plasmonic nanopores have raised attention as multidimensional biosensors with (SM) sensitivity. Here, we employ a gold plasmonic nanopore to monitor unfolding SM bovine serum albumin (BSA). The gradual collapse BSA structure induced by high bias voltages demonstrated through an increase fraction current blockade. Surface-enhanced Raman scattering (SERS) spectra provide structural evidence for protein unfolding, while optical force verified additional factor contributing deformation. effect on dwell time also investigated. present study reveals that offer observations which will drive further innovations analysis.

Language: Английский

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Effect of pyrimethanil on aβ42 aggregation mechanisms revealed at single entity level and molecular dynamic simulations

Biophysical Chemistry, Journal Year: 2025, Volume and Issue: 325, P. 107471 - 107471

Published: June 1, 2025

Language: Английский

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Tuning the Sensitivity of MoS₂ Nanopores: From Labeling to Labeling‐Free Detection of DNA Methylation

Small Methods, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 18, 2024

DNA methylation discrimination is often challenged by complicated pretreatment, insufficient sensitivity, and suboptimal accuracy. Here, single-molecule readout of reported using single-layer MoS

Language: Английский

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