MXene‐Based Photothermal‐Responsive Injectable Hydrogel Microsphere Modulates Physicochemical Microenvironment to Alleviate Osteoarthritis

Smart Medicine, Journal Year: 2025, Volume and Issue: 4(2)

Published: April 13, 2025

ABSTRACT Osteoarthritis (OA) is a physical lubrication microenvironment‐inadequate disease accompanied by sustained chronic chemical inflammation microenvironment and the progression of articular cartilage destruction. Despite promising OA treatment outcomes observed in enhancement inspired ball bearings to reduce friction support loads, therapeutic effect near‐infrared (NIR) irradiation‐based photothermal‐responsive controlled release “smart hydrogel microspheres” on remains unclear. Here, we prepared MXene/NIPIAM‐based injectable microspheres encapsulating diclofenac sodium using microfluidic system. Consequently, NIR demonstrate beneficial effects modulating microenvironment, laying foundation for application smart microsphere delivery systems loaded with bioactive factors (including agents, cells, factors) regulate multiple pathological microenvironments regenerative medicine.

Language: Английский

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Engineered Stem Cell Clusters for Extracellular Vesicles‐Mediated Gene Delivery to Rejuvenate Chondrocytes and Facilitate Chondrogenesis in Osteoarthritis Therapy

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: April 25, 2025

Abstract Gene therapy offers an ideal potential treatment strategy for osteoarthritis (OA). However, the safe and efficient delivery of therapeutic genes remains highly challenging because inactivation in direct miRNA, low transfection efficiency, a short half‐life. This study introduced gene using mesenchymal stem cells (MSCs) as platform achieved sustained via engineered MSCs‐derived extracellular vesicles (EVs). The miRNA‐874‐3p is combined with exosome‐targeting motif transfected into bone marrow (BMSCs). BMSCs motif+miR874 are then seeded onto hydrogel microspheres, creating BMSC /MS system OA treatment. In vitro experiments demonstrated that not only alleviated inflammation oxidative stress‐induced damage to chondrocytes by downregulating NF‐κB signaling pathway, thereby rejuvenating chondrocytes, but also promoted chondrogenesis inflammatory microenvironment. Furthermore, prolonged retention vivo, enabling gene, miRNA‐874‐3p, over extended duration. rat model, successfully delivered articular cartilage effectively degeneration. conclusion, this EVs‐mediated approach enables miRNA‐based viable alternative surgery provides novel option therapy.

Language: Английский

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Macrophage Membrane‐Biomimetic Multi‐Layered Nanoparticles Targeting Synovial Angiogenesis for Osteoarthritis Therapy

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 14, 2024

Abstract Osteoarthritis (OA) is an inflammatory and progressive joint disease characterized by angiogenesis‐mediated sustained, chronic, low‐grade synovitis. Anti‐angiogenesis emerging as a strategy for attenuating OA progression, but often compromised poor targeted drug delivery immune clearance. Recent studies have identified macrophages formed “protective barrier” in the lining layer (LL) of synovium, which blocked communication cavity sublining (SL) synovium. Inspired natural mimicry, macrophage membrane‐camouflaged explored to avoid Based on single cell RNA sequencing, CD34 + synovial cells are “sentinel cells” synovium angiogenesis. Consequently, antibody‐modified membrane constructed target new Hence, biomimetic multi‐layered nanoparticle (NP) developed that incorporates axitinib‐loaded poly(lactic‐co‐glycolic) acid (PLGA) with antibody modified (Atb@NP@Raw@CD34) specifically deliver axitinib (Atb) SL sustain inhibiting angiogenesis without elimination. It found Atb@NP@Raw@CD34 can pass through “barrier”, targeting cells, continuously releasing Atb anti‐angiogenesis Furthermore, vivo data demonstrated attenuate degeneration In conclusion, local injection presents promising approach clinically impeding progression.

Language: Английский

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Combating Cellular Aging: Frontiers in Biomaterials and Therapies

ACS Materials Letters, Journal Year: 2024, Volume and Issue: 7(1), P. 1 - 23

Published: Nov. 21, 2024

With the continuous increase in average life expectancy, population aging has become a significant health issue 21st century. The rising prevalence of age-related diseases brought tremendous pressure on social medical systems and economy. Against this backdrop, research anticellular hot spot field biomedicine, biomaterials are showing new trends potential area. Compared to drugs other traditional anti-aging strategies, can be personalized target characteristics different tissues, serving as drug delivery carriers enhance targeting, directly influence cellular process by modulating microenvironment. Furthermore, offer innovative approaches diagnosing building sophisticated models, which our understanding biology behind speed up advancement novel treatments. This review synthesizes latest advancements biomaterial for combating aging, exploring future prospects challenges.

Language: Английский

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