Polyphenol‐Regulated Bimetallic Artificial Metalloproteinases with Broad‐Spectrum RONS Scavenging Activities for Diabetic Wound Repair

Advanced Functional Materials, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Abstract Although numerous enzyme‐mimicking nanomaterials have been developed to regulate the highly reactive oxygen species (ROS)‐rich microenvironment of diabetic wounds for improving its healing, poor broad‐spectrum ROS‐scavenging capacity and biological toxicity resulting from side reactions still require improvement. To tackle these challenges, a polyphenol‐mediated copper‐bismuth bimetallic artificial metalloproteinases, CuBi‐TA@BSA, based on protein‐scaffold design strategy, is conceptually engineered. The Bi polyphenols can cooperatively enhance loading Cu ions their coordination within BSA scaffold. CuBi‐TA@BSA exhibits remarkable scavenging activity against various ROS via effectively donating electrons, as well reaction pathway specificity catalytic H 2 O decomposition without POD‐like activity. Moreover, in vitro vivo studies further confirm that protect cells oxidative damage, inhibit onset progression inflammation, promote wound healing. This work presents simple strategy biocatalysts scavenge accelerate wound‐healing process, also provides designing metalloproteinases antioxidative therapy.

Language: Английский

Inflammation‐Targeting Multienzyme Activity Carbon Dots Loaded with Methotrexate for Synergistic Immunotherapy in Rheumatoid Arthritis

Small, Journal Year: 2025, Volume and Issue: unknown

Published: April 4, 2025

In rheumatoid arthritis (RA), excessive reactive oxygen species (ROS) and chronic inflammation drive damage to the synovium, cartilage, bone. Developing precise synergistic therapy for RA is crucial improving remission rates. Here, carbon dots (CDs) with multienzyme activity inflammation-targeting capabilities are designed deliver methotrexate (MTX) treatment. Specifically, positively charged CDs porphyrin iron cores amino-functionalized surfaces synthesized simultaneously scavenge hydrogen peroxide, superoxide anions, hydroxyl radicals. Conjugation of MTX-loaded polyethylene glycol (CDs2-P@M) via Schiff base reaction significantly prolongs in vivo circulation time. collagen-induced rats, CDs2-P@M accumulates diseased joints, reducing ROS inflammatory cytokines, reprogramming macrophage phenotypes, inhibiting osteoclast activation, markedly symptoms. This approach targets microenvironment, minimizing MTX toxicity effectively reshaping immune homeostasis, halting tissue destruction, thus offering a new paradigm immunotherapy.

Language: Английский

ATP-enhanced platinum nanoparticle's peroxidase activity in alkaline environment for simplifying alkaline phosphatase detection

Sensing and Bio-Sensing Research, Journal Year: 2025, Volume and Issue: 48, P. 100798 - 100798

Published: April 29, 2025

Language: Английский