Advancements in Engineering Tetrahedral Framework Nucleic Acids for Biomedical Innovations DOI
Qin Fan,

Bing-Yao Sun,

Jie Chao

et al.

Small Methods, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 1, 2024

Tetrahedral framework nucleic acids (tFNAs) are renowned for their controllable self-assembly, exceptional programmability, and excellent biocompatibility, which have led to widespread application in the biomedical field. Beyond these features, tFNAs demonstrate unique chemical biological properties including high cellular uptake efficiency, structural bio-stability, tissue permeability, derived from distinctive 3D structure. To date, an extensive range of tFNA-based nanostructures intelligently designed developed various applications such as drug delivery, gene therapy, biosensing, engineering, among other emerging fields. In addition role delivery systems, also possess intrinsic that render them highly effective therapeutic agents treatment complex diseases, arthritis, neurodegenerative disorders, cardiovascular diseases. This dual functionality significantly enhances utility research, presenting valuable opportunities development next-generation medical technologies across diverse diagnostic platforms. Consequently, this review comprehensively introduces latest advancements field, with a focus on benefits nanoplatforms, inherent capabilities agents. Furthermore, current limitations, challenges, future perspectives explored.

Language: Английский

Circular Single‐Stranded DNA‐Based Artificial Nanoviruses Mitigate Colorectal Cancer Development DOI Open Access
Jinghao Wang, Pengfei Zhang,

Yonglian Huang

et al.

Small Methods, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 21, 2025

Abstract Colorectal cancer (CRC) remains a significant global health challenge, underscoring the need for innovative therapeutic strategies. Oncogenic miRNAs (oncomiRs) play biological role in initiation and progression of colorectal cancer. Inspired by cooperative mechanisms plant nanovirus, which employ multiple circular single‐stranded DNA (CssDNA) genomes, it is hypothesized that development delivery CssDNA to target oncomiRs would achieve benefits CRC. In this study, multi‐omics approach utilized identify key tumor suppressor genes (TSGs) their related implicated CRC, followed CssDNA, each loaded with miRNA binding sites targeting one oncomiR. When transfected into cells, these can effectively sequester corresponding restore expression TSGs, leading marked reduction CRC both vitro vivo. The findings highlight potential nanovirus‐inspired modulating miRNA‐mediated regulatory network This study lays groundwork non‐coding DNA‐based therapies broad implications treatment potentially other malignancies.

Language: Английский

Citations

0
Design and Characterization of DNA-Driven Condensates: Regulating Topology, Mechanical Properties, and Immunorecognition DOI Creative Commons
Elizabeth Skelly,

Christina J. Bayard,

Joel Jarusek

et al.

ACS Applied Materials & Interfaces, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Cells maintain spatiotemporal control over biochemical processes through the formation and dissolution of biomolecular condensates, dynamic membraneless organelles formed via liquid–liquid phase separation. Composed primarily proteins nucleic acids, these condensates regulate key cellular functions, their properties are influenced by concentration type molecules involved. The structural versatility challenges de novo design assembly with predefined properties. Through feedback between computational experimental approaches, we introduce a modular system for assembling using acid nanotechnology. By utilizing programmable oligonucleotides orthogonal synthesis methods, parameters, responsive behavior, immunorecognition products. Dissipative particle dynamics simulations predict some conditions to produce larger, well-defined compact, globular cores, while others result in smaller, more diffuse analogs. Fluorescence microscopy confirms findings microrheology demonstrates viscoelastic adaptability tested condensates. Nucleases trigger disruption structures, ethidium bromide intercalation protects from digestion. Immunostimulatory assays suggest condensate-specific activation IRF pathway cGAS-STING signaling. This study provides framework developing customizable various biological applications.

Language: Английский

Citations

0
Aminoguanidine-assembled functional DNA tetrahedron alleviates acute lung injury by targeting the cGAS-STING signaling pathway DOI Creative Commons
Yue Chen, Di Wu, Quan Li

et al.

Materials Today Bio, Journal Year: 2025, Volume and Issue: unknown, P. 101760 - 101760

Published: April 1, 2025

Language: Английский

Citations

0
Advancements in Engineering Tetrahedral Framework Nucleic Acids for Biomedical Innovations DOI
Qin Fan,

Bing-Yao Sun,

Jie Chao

et al.

Small Methods, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 1, 2024

Tetrahedral framework nucleic acids (tFNAs) are renowned for their controllable self-assembly, exceptional programmability, and excellent biocompatibility, which have led to widespread application in the biomedical field. Beyond these features, tFNAs demonstrate unique chemical biological properties including high cellular uptake efficiency, structural bio-stability, tissue permeability, derived from distinctive 3D structure. To date, an extensive range of tFNA-based nanostructures intelligently designed developed various applications such as drug delivery, gene therapy, biosensing, engineering, among other emerging fields. In addition role delivery systems, also possess intrinsic that render them highly effective therapeutic agents treatment complex diseases, arthritis, neurodegenerative disorders, cardiovascular diseases. This dual functionality significantly enhances utility research, presenting valuable opportunities development next-generation medical technologies across diverse diagnostic platforms. Consequently, this review comprehensively introduces latest advancements field, with a focus on benefits nanoplatforms, inherent capabilities agents. Furthermore, current limitations, challenges, future perspectives explored.

Language: Английский

Citations

1