A Stability Indicating HPLC Methodology Applying Quality by Design for the Concurrent Estimation of Luliconazole and Clobetasol Propionate in Cream Formulation

Separation Science Plus, Journal Year: 2025, Volume and Issue: 8(2)

Published: Feb. 1, 2025

ABSTRACT Antifungal agents containing azole ring (luliconazole [LULI]) and corticosteroid (clobetasol propionate [CLOB]) combination shows great therapeutic effects on skin infections by eliminating the fungus that is responsible for infection reducing inflammation of skin. The research work intended to create a liquid chromatographic methodology with stability study estimating LULI CLOB applying design experiment approach. were analyzed utilizing Hypersil BDS C 18 column (250 × 4.6 mm, 5 µm), using elution solvent comprising 0.1 M sodium phosphate buffer (pH 6.0) combined methanol in ratio 40:60 v/v. proposed method flow rate 1 mL/min detection wavelength 240 nm. A 3 2 full factorial was utilized optimize mobile phase. had undergone validation ICH strategies. force degradation conducted cream formulation both drugs. satisfactory linearity range found be 10–30 µg/mL, whereas 0.5–1.5 µg/mL CLOB. demonstrated good sensitivity, limit quantification 0.255 0.774 along 0.106 0.322 established precise (%RSD < 2) accurate (recovery between 98% 102%). studies aimed confirm efficient separation from their products. From chromatograms it has been any products, if present, do not interfere active constituents.

Language: Английский

Development of a Quality by Design-Based Ultra-Performance Liquid Chromatography Method for the Simultaneous Estimation of Casirivimab and Imdevimab with Greenness Metrics

Green Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 100248 - 100248

Published: March 1, 2025

Language: Английский

RP‐HPLC Method Development for Simultaneous Determination of Mesalamine and Quercetin in Pure Forms and in Solid Lipid Nanoparticle Formulations

Separation Science Plus, Journal Year: 2025, Volume and Issue: 8(4)

Published: April 1, 2025

ABSTRACT This study aimed to develop and validate a straightforward HPLC method for simultaneously estimating mesalamine quercetin in both pure forms solid lipid nanoparticle formulations. Following International Council Harmonisation Q2(R2) guidelines, the analytical was validated. Furthermore, this optimized applied measure percentage entrapment efficiency vitro drug release central composite design‐based The chromatogram showed peak of at retention times 2.28 8.79 min, respectively. For mesalamine, limits detection quantification were found be 1.64 4.97 µg/mL, Whereas quercetin, 2.10 µg/mL limit 6.36 nanoparticles 85.27 ± 1.74% 78.62 2.38%, indicated 98.83 2.94% 96.56 1.92% from over 24 h. results demonstrated that developed accurate, precise, robust, maintaining its reliability despite minor changes flow rate wavelength. Consequently, suitable various

Language: Английский