Quantification of Biomolecular Condensate Volume Reveals Network Swelling and Dissolution Regimes during Phase Transition DOI Creative Commons
Iris B. A. Smokers, Evan Spruijt

Biomacromolecules, Journal Year: 2024, Volume and Issue: 26(1), P. 363 - 373

Published: Dec. 2, 2024

Accurate determination of biomolecular condensate volume reveals that destabilization condensates can lead to either swelling or shrinking condensates, giving fundamental insights into the regulation cellular condensates. Determination and coacervate protocells is essential investigate their precise composition impact on (bio)chemical reactions are localized inside It not a straightforward task, as have tiny volumes, highly viscous, prone wetting. Here, we examine different strategies determine introduce two new methods, with which volumes 1 μL less (volume fraction 0.4%) be determined standard deviation 0.03 μL. Using these show depends degree physical cross-linking. These observations supported by Flory–Huggins theory profound effects in cell biology.

Language: Английский

Molecular determinants of condensate composition DOI Creative Commons
Alex S. Holehouse, Simon Alberti

Molecular Cell, Journal Year: 2025, Volume and Issue: 85(2), P. 290 - 308

Published: Jan. 1, 2025

Language: Английский

Citations

4
Chemically Triggered Reactive Coacervates Show Life-Like Budding and Membrane Formation DOI Creative Commons
Sudeep Koppayithodi, Nishant Singh

Journal of the American Chemical Society, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 28, 2025

Phase-separated coacervates can enhance reaction kinetics and guide multilevel self-assembly, mimicking early cellular evolution. In this work, we introduce "reactive" complex that undergo chemically triggered self-immolative transformations, directing the self-assembly of products within their matrix. These self-assemblies then evolve to show life-like properties such as budding membrane formation. We find coacervate composition critically influences rates product distribution guides hierarchical self-assembly. This work showcases a versatile platform influence pathways for controlled supramolecular synthesis self-organization in confined spaces.

Language: Английский

Citations

0
Toward Design Principles for Biomolecular Condensates for Metabolic Pathways DOI Creative Commons
Alain A.M. André,

Nikita Rehnberg,

Ankush Garg

et al.

Advanced Biology, Journal Year: 2025, Volume and Issue: unknown

Published: April 7, 2025

Abstract Biology uses membrane‐less organelles or biomolecular condensates as dynamic reaction compartments that can form dissolve to regulate biochemical pathways. This has led a flurry of research aiming design new synthetic function crucibles for enzymes and cascades in biotechnology. The mechanisms by which condensate enhance multistep processes including mass action, tuning the chemical environment, scaffolding metabolic channelling is reviewed. These are not inherently beneficial rate enzymatic but also inhibit reaction. Similarly, some aspects likely intrinsically inhibitory retardation diffusion, where net effect will be trade‐off between stimulatory effects. It discussed generalizable conclusions drawn so far how close it principles enzyme microbial cell factories reactions enhanced type suited

Language: Английский

Citations

0
Quantification of Biomolecular Condensate Volume Reveals Network Swelling and Dissolution Regimes during Phase Transition DOI Creative Commons
Iris B. A. Smokers, Evan Spruijt

Biomacromolecules, Journal Year: 2024, Volume and Issue: 26(1), P. 363 - 373

Published: Dec. 2, 2024

Accurate determination of biomolecular condensate volume reveals that destabilization condensates can lead to either swelling or shrinking condensates, giving fundamental insights into the regulation cellular condensates. Determination and coacervate protocells is essential investigate their precise composition impact on (bio)chemical reactions are localized inside It not a straightforward task, as have tiny volumes, highly viscous, prone wetting. Here, we examine different strategies determine introduce two new methods, with which volumes 1 μL less (volume fraction 0.4%) be determined standard deviation 0.03 μL. Using these show depends degree physical cross-linking. These observations supported by Flory–Huggins theory profound effects in cell biology.

Language: Английский

Citations

0