Standard Doses of Cholecalciferol Reduce Glucose and Increase Glutamine in Obesity-Related Hypertension: Results of a Randomized Trial

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(6), P. 3416 - 3416

Published: March 18, 2024

In arterial hypertension, the dysregulation of several metabolic pathways is closely associated with chronic immune imbalance and inflammation progression. With time, these disturbances lead to development progressive disease end-organ involvement. However, influence cholecalciferol on as a possible mechanism its immunomodulatory activity in obesity-related hypertension not known. phase 2, randomized, single-center, 24-week trial, we evaluated, secondary outcome, serum metabolome 36 age- gender-matched adults vitamin D deficiency, before after supplementation therapy along routine medication. The defined endpoint was assessment circulating metabolites using nuclear magnetic resonance-based metabolomics approach. Univariate multivariate analyses were used evaluate systemic alterations caused by cholecalciferol. comparison normotensive controls, hypertensive patients presented overall decreased expression amino acids (p < 0.05), including ketogenic glucogenic properties well aromatic acids. Following supplementation, increases observed glutamine 0.001) histidine levels other remaining unaffected. Glucose 0.05) acetate 24 weeks group taking supplement, changes saturation fatty also observed, suggesting role liposoluble lipid metabolism. Long-term chronically obese overweight hypertensives induced blood metabolome, which reflected metabolism may have fostered new microenvironment for cell proliferation biology. Of note, increased availability be relevant different T-cell subsets.

Language: Английский

The effect of intestinal flora metabolites on macrophage polarization

Heliyon, Journal Year: 2024, Volume and Issue: 10(15), P. e35755 - e35755

Published: Aug. 1, 2024

Intestinal flora metabolites played a crucial role in immunomodulation by influencing host immune responses through various pathways. Macrophages, as type of innate cell, were essential chemotaxis, phagocytosis, inflammatory responses, and microbial elimination. Different macrophage phenotypes had distinct biological functions, regulated diverse factors mechanisms. Advances intestinal sequencing metabolomics have enhanced understanding how affect functions. These varying effects on polarization different mechanisms influence. This study summarized the impact gut microbiota phenotype function, along with underlying associated produced flora.

Language: Английский

Hsa_Circ_0008035 drives immune evasion of gastric cancer via promoting EXT1-mediated nuclear translocation of PKM2

Translational Oncology, Journal Year: 2024, Volume and Issue: 48, P. 102004 - 102004

Published: July 24, 2024

Circular RNAs (circRNAs) have been reported to be associated with the malignant phenotypes of cancer. However, role and underlying mechanism hsa_Circ_0008035 in colorectal cancer (CRC) remains unclear. In this study, we elucidated pivotal hsa_circ_0008035 gastric progression immune evasion. Elevated levels patient serum correlated positively disease advancement, including tumor stages lymph node metastasis. Functional analyses revealed a negative association between CD8+ T cell number function. Mechanistically, encoded novel protein EXT1-219aa, suppressing EXT1 phosphorylation expression. Additionally, regulated pyruvate metabolism by influencing nucleus localization PKM2. The identified EXT1/PKM2 axis further underscored intricate regulatory mechanisms orchestrated cancer, offering potential diagnostic therapeutic implications ongoing pursuit targeted therapies for patients.

Language: Английский