Battling pain from osteoarthritis: causing novel cell death DOI Creative Commons
Yuheng Zhang,

Huaqiang Tao,

Liyuan Zhang

et al.

Acta Biochimica et Biophysica Sinica, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 1, 2024

Osteoarthritis (OA) is a significant contributor to pain and disability worldwide. Pain the main complaint of OA patients attending clinic has large impact on their quality life economic standards. However, existing treatments for OA-related have not been shown achieve good relief. The focus preventing slowing progression so that problem can be resolved. caused by complex, with nature, location, duration, intensity changing as disease progresses. Previous research highlighted role various forms cell death, such apoptosis necrosis, in OA. Emerging studies identified additional novel pyroptosis, ferroptosis, necroptosis are linked Different types death contribute tissue damage impacting inflammatory responses, reactive oxygen species (ROS) production, calcium ion levels, ultimately leading development pain. Evidence suggests targeting could help alleviate patients. This review delves into complex mechanisms pain, explores relationship between different modes proposes viable strategy treatment these conditions, goal providing scientific references future drugs.

Language: Английский

Ecdysterone Alleviates Atherosclerosis by Inhibiting NCF2 and Inhibiting Ferroptosis Mediated by the PI3K/Akt/Nrf2 Pathway DOI Creative Commons
Zhenyu Wang,

Fengchao Wu,

Yan Ju

et al.

Journal of Cellular and Molecular Medicine, Journal Year: 2025, Volume and Issue: 29(5)

Published: March 1, 2025

Ecdysterone (Ecd), an active ingredient in trianthema, has a strong anti-inflammatory effect. This study aimed to explore the potential mechanism by which Ecd improves atherosclerosis (AS). Here, we systematically investigated of human umbilical vein endothelial cells (HUVECs) treated with oxidised low-density lipoprotein (ox-LDL). In ox-LDL-treated HUVECs, promoted HUVEC viability as well inhibited ferroptosis and secretion inflammatory factors (TNF-α, IL-6 IL-1β). addition, expression neutrophil cytoplasmic factor 2 (NCF2) triggered PI3K/AKT/Nrf2 signalling pathway, thereby alleviating increase HUVECs. More importantly, constructed AS mouse model feeding ApoE-/- mice high-fat diet found that treatment alleviated vasculopathy arterial vivo, could be reversed overexpression NCF2. Overall, this showed protective effect on is mainly achieved inhibiting NCF2 activating PI3K/Akt/Nrf2 pathway inhibit ferroptosis. Therefore, may effective drug improve ferroptosis-induced inflammation.

Language: Английский

Citations

1
Exploring the Therapeutic Potential of MIR‐140‐3p in Osteoarthritis: Targeting CILP and Ferroptosis for Novel Treatment Strategies DOI Creative Commons
Feng Ma, Lexin Wang, Hao Chi

et al.

Cell Proliferation, Journal Year: 2025, Volume and Issue: unknown

Published: March 5, 2025

Osteoarthritis (OA) is a prevalent and debilitating joint disorder that affects millions of individuals worldwide, severely impairing mobility, independence, quality life. Emerging evidence suggests ferroptosis critical factor in OA pathogenesis. However, its precise involvement underlying mechanisms remain poorly understood. In this study, we first identified cartilage intermediate layer protein (CILP) mediates the regulation ferroptosis-related genes through hdWGCNA analysis combined with single-cell RNA sequencing. Further investigation revealed significant upregulation CILP expression C28/I2 cells under LPS induction. Mechanistically, bioinformatics differentially expressed miRNAs; qRT-PCR dual-luciferase experiment miR-140-3p was downregulated directly targets CILP. Experimental data further demonstrated regulates ferroptosis, inflammation, oxidative stress by targeting These findings offer valuable insights into molecular miR-140-3p/CILP axis regulating stress, thus providing foundation for developing therapeutic strategies OA.

Language: Английский

Citations

0
Bioinformatics analysis techniques identify the ferroptosis-related gene MYC as a potential therapeutic target for spinal cord injury: an observational study based on the GEO database DOI

Xiaowei Zha

Advanced technology in neuroscience ., Journal Year: 2025, Volume and Issue: 2(2), P. 59 - 71

Published: April 8, 2025

JOURNAL/atin/04.03/02274269-202506000-00001/figure1/v/2025-04-06T104049Z/r/image-tiff Spinal cord injury is a destructive disease with limited effective treatment options. Ferroptosis, form of regulated cell death, has been suggested to be related the pathogenesis spinal injury. This study aimed elucidate prognostic value and therapeutic potential ferroptosis-related genes in using advanced bioinformatics techniques. We retrieved datasets from GEO database identified differentially expressed between control groups. These were intersected identify genes. A hub gene was through cytoHubba plugin Cytoscape, miRNA–mRNA–transcription factor drug–biomarker networks constructed reveal molecular targets compounds. The results revealed highly upregulated gene, MYC , group. Subsequent analyses 30 miRNAs, transcription factors, drugs as associated this for findings suggest that analysis techniques provide new insights into role injury, highlighting biomarker target lays foundation personalized facilitating development strategies tailored unique characteristics individual patients.

Language: Английский

Citations

0
ADORA2B promotes proliferation and migration in head and neck squamous cell carcinoma and is associated with immune infiltration DOI Creative Commons

Pian Li,

Kai Pang,

Shuanghui Chen

et al.

BMC Cancer, Journal Year: 2025, Volume and Issue: 25(1)

Published: April 12, 2025

Adenosine A2B receptor (ADORA2B), a G protein-coupled receptor, is implicated in tumor progression and immune regulation various cancers. However, its specific role head neck squamous cell carcinoma (HNSC) remains largely unexplored. This study aims to elucidate the expression profile, prognostic value, modulatory role, therapeutic potential of ADORA2B HNSC. Comprehensive bioinformatics analyses were performed using TCGA GEO datasets evaluate expression, clinical correlations, significance Weighted gene co-expression network analysis (WGCNA) functional enrichment conducted explore ADORA2B-associated pathways. Immune infiltration was assessed via ESTIMATE single-sample set (ssGSEA). checkpoint blockade (ICB) therapy sensitivity drug analyzed IMvigor210 NCI-60 databases, respectively. In vitro experiments, including siRNA-mediated knockdown, CCK-8 assays, colony formation, wound healing validate oncogenic ADORA2B. significantly overexpressed HNSC tissues compared adjacent normal tissues, correlated with advanced stage as well poor overall survival (OS) progression-free (PFS). Functional revealed significant downregulation immune-related pathways high groups. High associated more immunosuppressive microenvironment (TME), characterized by lower stromal scores reduced infiltration. Immunotherapy response demonstrated that patients exhibited poorer outcomes following ICB therapy. Drug identified several agents, Ixazomib citrate, Masitinib, others, candidates for patients. experiments confirmed knockdown inhibited proliferation, migration, underscoring critical progression. key driver HNSC, contributing an TME. Its prognosis immunotherapy efficacy. Targeting may enhance overcome treatment resistance, highlighting diagnostic, prognostic, biomarker.

Language: Английский

Citations

0
Identification of Therapeutic Targets for Hyperuricemia: Systematic Genome-Wide Mendelian Randomization and Colocalization Analysis DOI Creative Commons

Na Chen,

Leilei Gong, Li Zhang

et al.

Biomedicines, Journal Year: 2025, Volume and Issue: 13(5), P. 1022 - 1022

Published: April 23, 2025

Background: At present, there are still limitations and challenges in the treatment of hyperuricemia (HUA). Mendelian randomization (MR) has been widely used to identify new therapeutic targets. Therefore, we conducted a systematic druggable genome-wide MR explore potential targets drugs for HUA. Methods: We integrated genome data; blood, kidney, intestinal expression quantitative trait loci (eQTLs); HUA-associated association study (GWAS) data analyze causal relationships between drug target genes HUA using method. Summary-data-based (SMR) analysis Bayesian colocalization were assess causality. In addition, phenome-wide studies, protein network construction, enrichment significant evaluate their biological functions side effects. Finally, performed prediction molecular docking targeting these treatment. Results: Overall, identified 22 significantly associated with through MR, SMR, analyses. Among them, two prior (ADORA2B NDUFC2) reached statistically levels at least tissues intestine. Further results from studies revealed that no effects ADORA2B or NDUFC2. Moreover, screened 15 could serve as candidates development. Conclusions: This provides genetic evidence supporting benefits treatment, offering insights into development targeted

Language: Английский

Citations

0
To verify the biological characteristics of disulfidptosis associated gene ADORA2B in esophageal cancer DOI Creative Commons
Yixiao Cui,

Yuhan Deng,

Zhenhua Wu

et al.

BMC Gastroenterology, Journal Year: 2025, Volume and Issue: 25(1)

Published: May 19, 2025

Language: Английский

Citations

0
Battling pain from osteoarthritis: causing novel cell death DOI Creative Commons
Yuheng Zhang,

Huaqiang Tao,

Liyuan Zhang

et al.

Acta Biochimica et Biophysica Sinica, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 1, 2024

Osteoarthritis (OA) is a significant contributor to pain and disability worldwide. Pain the main complaint of OA patients attending clinic has large impact on their quality life economic standards. However, existing treatments for OA-related have not been shown achieve good relief. The focus preventing slowing progression so that problem can be resolved. caused by complex, with nature, location, duration, intensity changing as disease progresses. Previous research highlighted role various forms cell death, such apoptosis necrosis, in OA. Emerging studies identified additional novel pyroptosis, ferroptosis, necroptosis are linked Different types death contribute tissue damage impacting inflammatory responses, reactive oxygen species (ROS) production, calcium ion levels, ultimately leading development pain. Evidence suggests targeting could help alleviate patients. This review delves into complex mechanisms pain, explores relationship between different modes proposes viable strategy treatment these conditions, goal providing scientific references future drugs.

Language: Английский

Citations

0