Thiacloprid Exposure Induces Oxidative Stress, Endoplasmic Reticulum Stress, and Apoptosis in the Liver of Mauremys reevesii

Ecology and Evolution, Journal Year: 2025, Volume and Issue: 15(2)

Published: Feb. 1, 2025

ABSTRACT Among neonicotinoid insecticides, thiacloprid (THI) is extensively utilized in agricultural practices, which poses a potential toxicity risk to aquatic fauna. Turtles, integral ecosystems, have not yet been comprehensively assessed for their vulnerability THI exposure. In this study, we aimed evaluate the effects of on oxidative stress, endoplasmic reticulum stress (ERS), and apoptosis turtles. We categorized Mauremys reevesii into three groups: control group two experimental groups exposed environmentally relevant (4.5 μg/mL) high (15 mg/mL) concentrations THI, respectively. Transcriptome analysis revealed that genes significantly associated with elimination superoxide radicals, organelle inner membrane functions, peroxiredoxin activity, apoptotic pathways were abundantly expressed high‐concentration group. Notably, exposure led marked increase glutathione peroxidase (GPX) dismutase (SOD) activities, whereas catalase (CAT) activity declined malondialdehyde (MDA) levels rose, indicating presence stress. Moreover, upregulated expression ER marker GRP78. Simultaneously, mRNA pivotal unfolded protein response genes, including AFT6, AFT4, IRE1α, CHOP, XBP1, eIF2α, elevated Furthermore, activated activities caspase‐3, caspase‐8, caspase‐9 enzymes liver tissue. The anti‐apoptotic gene Bcl‐2 was downregulated, pro‐apoptotic Bax caspase‐3 upregulated, leading an hepatic cells following Collectively, our study indicates can induce damage turtles through promotion ERS, apoptosis. These findings gain deeper understanding toxic keystone species thereby improving overall environmental impacts.

Language: Английский

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Hepatoprotective Effects of Royal Jelly Against Vincristine-Induced Hepatotoxicity in Rats: A Biochemical and Molecular Study

Life, Journal Year: 2025, Volume and Issue: 15(3), P. 459 - 459

Published: March 14, 2025

Vincristine (VCR) is a chemotherapeutic agent classified as vinca alkaloid. Royal jelly (RJ) significant bee product produced by worker bees, characterized its high protein content. This study aims to investigate the protective effects of RJ against VCR-induced liver damage. VCR was intraperitoneally administered at dose 0.1 mg/kg body weight (b.w.) and orally doses 150 300 b.w. Both treatments were applied rats on days 1–6 9–14. The composition analyzed using LC-MS/MS, revealing presence 15 different phytochemical compounds with strong antioxidant properties. Serum samples obtained from for ALT, ALP, AST levels. While these enzyme levels significantly elevated in group, notable reduction observed following administration. Additionally, SOD, CAT, GPx, GSH parameters, along MDA levels, evaluated tissue samples. results indicated decrease activities/levels an increase group. Furthermore, ELISA used assess JAK2, STAT3, mTOR/PI3K/AKT signaling pathways. administration led JAK2 STAT3 In addition, mRNA transcription inflammation (NF-κB, TNF-α, IL-1β), endoplasmic reticulum (ER) stress (IRE-1, GRP78, PERK, ATF-6), autophagy markers (LC3A LC3B) examined. A inflammation, ER stress, autophagy-related VCR-treated Lastly, expression Bax, Bcl-2, Caspase-3, NF-κB evaluated. treatment increased Caspase 3, whereas Bcl-2 decreased. However, administration, all parameters reversed, demonstrating improvements. conclusion, findings suggest that may exert effect

Language: Английский

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Antioxidant Potential and Phytochemical Profile of Althaea (Hatmi) and Hibiscus Flower Extracts: A Comprehensive Analysis

Türk doğa ve fen dergisi :/Türk doğa ve fen dergisi, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 20, 2024

In this study, the bioactive components and antioxidant properties of Althaea (Hatmi) Hibiscus plants were assessed using various methods. Both aqueous ethanol extracts these yielded distinct effective results. Antioxidant activity was evaluated 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), Ferric Reducing Power (FRAP) assays at concentrations 25, 50, 75, 100 mg/mL. Hatmi extracts, both aqueous, exhibited high DPPH activity, particularly 75 mg/mL, while showed a linear increase in with concentration, reaching 2000 µM Trolox Equivalent (TE) /g dry weight (DW) ABTS assays, lower more effective, but higher greater activity. FRAP results indicated 2700 TE/g DW concentrations. Phenolic analysis revealed levels apigenin 7-glucoside, hesperidin, caffeic acid Hatmi, contained significant amounts chlorogenic quercetin. Gas Chromatography-Mass Spectrometry (GC-MS) that had abundance volatile organic compounds compared to Hibiscus.

Language: Английский

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