Sensitivity to photoperiod is a complex trait in Camelina sativa DOI Creative Commons
Bryan A. Ramirez‐Corona, Eric A. Seagren, Carissa Sherman

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 29, 2024

Summary Day neutrality, or insensitivity to photoperiod (day length), is an important domestication trait in many crop species. Although the oilseed Camelina sativa has been cultivated since Neolithic era, day-neutral accessions have yet be described. We sought leverage genetic diversity existing germplasms identify C. with low sensitivity for future engineering of this trait. quantified variation response across 161 by measuring hypocotyl length four-day-old seedlings grown long-day and short-day conditions, finding wide response. Similarly, soil-grown adult plants from selected showed several traits; however, responses seedling traits were not correlated, suggesting complex mechanistic underpinnings. RNA-seq experiments reference accession Licalla identified differentially regulated Arabidopsis syntelogs involved response, including COL2, FT, LHY WOX4 , expression these genes did correlate differences their sensitivity. Taken together, we show that all tested some degree likely complex, involving separable traits. Significance Statement neutrality (photoperiod insensitivity) a common domesticated crops; ancient remained photoperiod-sensitive, which limits seed yields. Here, conserved cultivars, albeit different degrees, establish trait, will require achieve day neutrality.

Language: Английский

Homozygosity mapping identified loci and candidate genes responsible for freezing tolerance in Camelina sativa DOI Creative Commons
TM Shaikh, Mukhlesur Rahman, Timothy P. L. Smith

et al.

The Plant Genome, Journal Year: 2023, Volume and Issue: 16(2)

Published: March 9, 2023

Homozygosity mapping is an effective tool for detecting genomic regions responsible a given trait when the phenotype controlled by limited number of dominant or co-dominant loci. Freezing tolerance major attribute in agricultural crops such as camelina. Previous studies indicated that freezing differences between tolerant (Joelle) and susceptible (CO46) variety camelina were small genes. We performed whole genome homozygosity to identify markers candidate genes difference these two genotypes. A total 28 F3 RILs sequenced ∼30× coverage, parental lines >30-40× coverage with Pacific Biosciences high fidelity technology 60× using Illumina sequencing. Overall, about 126k homozygous single nucleotide polymorphism identified differentiate both parents. Moreover, 617 also families fixed tolerance/susceptibility. All mapped contigs forming contiguous stretch chromosome 11. The detected 9 blocks among selected 22 strong similarity near blocks. Two differentially expressed during cold acclimation largest block contained cold-regulated plant thionin putative rotamase cyclophilin 2 gene previously associated resistance arabidopsis (Arabidopsis thaliana). second contains several cysteine-rich RLK receptor serine/threonine kinase gene. hypothesize one more may be primarily varieties.

Language: Английский

Citations

3
Genome-scale investigation and identification of variations associated with early flowering based on whole genome resequencing and transcriptome integrated analysis in tree peony DOI
Lili Guo, Yuying Li, Lei Yang

et al.

Scientia Horticulturae, Journal Year: 2022, Volume and Issue: 310, P. 111695 - 111695

Published: Nov. 26, 2022

Language: Английский

Citations

4
Assessment of molecular diversity in doubled haploid lines of camelina (Camelina sativa(L.) Crantz), as a new emerging oil crop DOI

Mozafar Sadeghikian,

Abdollah Najaphy, Danial Kahrizi

et al.

Plant Genetic Resources, Journal Year: 2022, Volume and Issue: 20(4), P. 249 - 254

Published: Aug. 1, 2022

Abstract Camelina ( sativa (L.) Crantz), an oilseed crop, belongs to the Brassicaceae family. Two unique features of camelina in comparison with main oil crops are adaptation different environments and also its composition. The development doubled haploid plants is one essential methods for crop improvement. study genetic diversity important step planning breeding programmes. This research was conducted evaluate variation 81 lines obtained from 15 crosses by inter simple sequence repeat (ISSR) markers. total number amplified bands 243, which 239 (98.3%) showed polymorphism. percentage polymorphic varied between 93.75 100. size ranged 50 1700 base pairs. informative ISSRs were identified estimating marker features: polymorphism information content, effective multiplex ratio, index resolving power. Three markers had higher power values (9.88, 8.5 7.46) most identify lines. Cluster analysis based on complete algorithm divided into five groups, indicating relatively clear configuration geographic distribution patterns parents Principal coordinate classified six groups. ISSR detected high reveal findings this research, along characterization biochemical traits lines, can improve programmes achieve high-yielding varieties better content.

Language: Английский

Citations

3
Allopolyploidy expanded gene content but not pangenomic variation in the hexaploid oilseedCamelina sativa DOI Creative Commons
Kevin A. Bird, Jordan R. Brock, Paul Grabowski

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 16, 2024

ABSTRACT Ancient whole-genome duplications (WGDs) are believed to facilitate novelty and adaptation by providing the raw fuel for new genes. However, it is unclear how recent WGDs may contribute evolvability within polyploids. Hybridization accompanying some combine divergent gene content among diploid species. Some theory evidence suggest that polyploids have a greater accumulation tolerance of presence-absence genomic structural variation, but what extent either true. To test polyploidy influence pangenomic we sequenced, assembled, annotated twelve complete, chromosome-scale genomes Camelina sativa , an allohexaploid biofuel crop with three distinct subgenomes. Using comparative analyses, characterized variation both between We found over 75% ortholog clusters core in <10% sequence space was affected rearrangements. In contrast, 19% were unique one subgenome, majority these Camelina-specific (no Arabidopsis). identified inversion vernalization requirements winter-type Camelina, enrichment genes enzymatic processes related seed oil quality Camelina’s glucosinolate profile. Genes traits exhibited little variation. Our results reveal minimal this species, instead show hybridization accompanied WGD benefit merging diverged different

Language: Английский

Citations

0
Sensitivity to photoperiod is a complex trait in Camelina sativa DOI Creative Commons
Bryan A. Ramirez‐Corona, Eric A. Seagren, Carissa Sherman

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 29, 2024

Summary Day neutrality, or insensitivity to photoperiod (day length), is an important domestication trait in many crop species. Although the oilseed Camelina sativa has been cultivated since Neolithic era, day-neutral accessions have yet be described. We sought leverage genetic diversity existing germplasms identify C. with low sensitivity for future engineering of this trait. quantified variation response across 161 by measuring hypocotyl length four-day-old seedlings grown long-day and short-day conditions, finding wide response. Similarly, soil-grown adult plants from selected showed several traits; however, responses seedling traits were not correlated, suggesting complex mechanistic underpinnings. RNA-seq experiments reference accession Licalla identified differentially regulated Arabidopsis syntelogs involved response, including COL2, FT, LHY WOX4 , expression these genes did correlate differences their sensitivity. Taken together, we show that all tested some degree likely complex, involving separable traits. Significance Statement neutrality (photoperiod insensitivity) a common domesticated crops; ancient remained photoperiod-sensitive, which limits seed yields. Here, conserved cultivars, albeit different degrees, establish trait, will require achieve day neutrality.

Language: Английский

Citations

0