An RNA ligase partner for the prokaryotic protein-only RNase P: insights into the functional diversity of RNase P from genome mining DOI Creative Commons
R. Seshadri, Venkat Gopalan

mBio, Journal Year: 2025, Volume and Issue: unknown

Published: April 29, 2025

ABSTRACT RNase P can use either an RNA- or a protein-based active site to catalyze 5′-maturation of transfer RNAs (tRNAs). This distinctive attribute in the biocatalytic repertoire raises questions about underlying evolutionary driving forces, especially if each variant somehow affords selective advantage under certain conditions. Upon mining all publicly available prokaryotic genomes and examining gene co-occurrence, we discovered that RNA ligase with circularization activity was significantly overrepresented contain protein form P. unexpected linkage inspires testable ideas understand bases for scenarios might favor variants different architectures/make-up.

Language: Английский

The Influence of Chitosan Derivatives in Combination with Bacillus subtilis Bacteria on the Development of Systemic Resistance in Potato Plants with Viral Infection and Drought DOI Creative Commons

Liubov Yarullina,

J. N. Kalatskaja, Vyacheslav Tsvetkov

et al.

Plants, Journal Year: 2024, Volume and Issue: 13(16), P. 2210 - 2210

Published: Aug. 9, 2024

Viral diseases of potatoes are among the main problems causing deterioration in quality tubers and loss yield. The growth development potato plants largely depend on soil moisture. Prevention strategies require comprehensive protection against pathogens abiotic stresses, including modeling beneficial microbiome agroecosystems combining microorganisms immunostimulants. Chitosan its derivatives have great potential for use agricultural engineering due to their ability induce plant immune responses. effect chitosan conjugate with caffeic acid (ChCA) combination Bacillus subtilis 47 transcriptional activity PR protein genes changes proteome during virus Y (PVY) infection drought was studied. mechanisms increasing resistance PVY lack moisture associated activation transcription encoding proteins: protective (PR-1), chitinase (PR-3), thaumatin-like (PR-5), protease inhibitor (PR-6), peroxidase (PR-9), ribonuclease (PR-10), as well qualitative quantitative proteome. revealed expression marker systemic acquired induced under influence combined treatment B. indicate that, plants, formation viral conditions proceeds synergistically. By two-dimensional electrophoresis S. tuberosum leaf proteins followed by MALDI-TOF analysis, 10 were identified, content composition which differed depending experiment variant. In infected treated ChCA, synthesis proteinaceous RNase P 1 oxygen-evolving enhancer 2 enhanced normal humidity, 20 kDa chaperonin TMV N-like coat detected, intensively accumulated leaves Y-virus. ChCA reduced these leaves, subtilis, not detected at all, both humidity moisture, suggests promising bacteria regulation resistance.

Language: Английский

Citations

5
An RNA ligase partner for the prokaryotic protein-only RNase P: insights into the functional diversity of RNase P from genome mining DOI Creative Commons
R. Seshadri, Venkat Gopalan

mBio, Journal Year: 2025, Volume and Issue: unknown

Published: April 29, 2025

ABSTRACT RNase P can use either an RNA- or a protein-based active site to catalyze 5′-maturation of transfer RNAs (tRNAs). This distinctive attribute in the biocatalytic repertoire raises questions about underlying evolutionary driving forces, especially if each variant somehow affords selective advantage under certain conditions. Upon mining all publicly available prokaryotic genomes and examining gene co-occurrence, we discovered that RNA ligase with circularization activity was significantly overrepresented contain protein form P. unexpected linkage inspires testable ideas understand bases for scenarios might favor variants different architectures/make-up.

Language: Английский

Citations

0