Cell cycle–independent integration of stress signals by Xbp1 promotes Non-G1/G0 quiescence entry

The Journal of Cell Biology, Journal Year: 2021, Volume and Issue: 221(1)

Published: Oct. 25, 2021

Cellular quiescence is a nonproliferative state required for cell survival under stress and during development. In most quiescent cells, proliferation stopped in reversible of low Cdk1 kinase activity; many organisms, however, states with high-Cdk1 activity can also be established through still uncharacterized or developmental mechanisms. Here, we used microfluidics approach coupled to phenotypic classification by machine learning identify pathways associated starvation-triggered Saccharomyces cerevisiae. We found that low- shared core stress-associated processes, such as autophagy, protein aggregation, mitochondrial up-regulation, but differed the nuclear accumulation transcription factors Xbp1, Gln3, Sfp1. The decision between was controlled cycle-independent which acted time-delayed integrator duration stimuli. Our results show how stress-activated promote cellular outside G1/G0.

Language: Английский

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Novel B-DNA dermatophyte assay for demonstration of canonical DNA in dermatophytes: Histopathologic characterization by artificial intelligence

Clinics in Dermatology, Journal Year: 2024, Volume and Issue: 42(3), P. 233 - 258

Published: Jan. 6, 2024

Language: Английский

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Old yeasts, young beer—The industrial relevance of yeast chronological life span

Yeast, Journal Year: 2021, Volume and Issue: 38(6), P. 339 - 351

Published: May 12, 2021

Abstract Much like other living organisms, yeast cells have a limited life span, in terms of both the maximal length time cell can stay alive (chronological span) and number divisions it undergo (replicative span). Over past years, intensive research revealed that span depends on genetic background environmental factors. Specifically, presence stress factors, reactive oxygen species, availability nutrients profoundly impact signaling cascades involved response to these including target rapamycin (TOR) cyclic adenosine monophosphate (cAMP)/protein kinase A (PKA) pathways, play central role. Interestingly, also has direct implications for its use industrial processes. In beer brewing, example, inoculation finished with live cells, process called “bottle conditioning” helps improve product's shelf by clearing undesirable carbonyl compounds such as furfural 2‐methylpropanal cause staling. However, this effect reductive metabolism is thus inherently cells' chronological span. Here, we review mechanisms underlying yeast. We discuss how insight connects observations ultimately opens new routes towards superior yeasts help contribute more sustainable industry.

Language: Английский

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Tup1 is critical for transcriptional repression in Quiescence in S. cerevisiae

PLoS Genetics, Journal Year: 2022, Volume and Issue: 18(12), P. e1010559 - e1010559

Published: Dec. 21, 2022

Upon glucose starvation, S . cerevisiae shows a dramatic alteration in transcription, resulting wide-scale repression of most genes and activation some others. This coincides with an arrest cellular proliferation. A subset such cells enters quiescence, reversible non-dividing state. Here, we demonstrate that the conserved transcriptional corepressor Tup1 is critical for after depletion. We show Tup1-Ssn6 binds new targets upon depletion, where it remains as enter G0 phase cell cycle. In addition, represses variety metabolism transport genes. explored how mediated accomplished demonstrated coordinates Rpd3L complex to deacetylate H3K23. found Isw2 affect nucleosome positions at transporter HXT family during G0. Finally, microscopy revealed quarter deletion contain multiple DAPI puncta. Taken together, these findings role reprogramming response environmental cues leading quiescent

Language: Английский

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Metabolic dynamics during the cell cycle

Current Opinion in Systems Biology, Journal Year: 2022, Volume and Issue: 30, P. 100415 - 100415

Published: Feb. 11, 2022

While we have a solid understanding of the cell biological and biochemical control aspects eukaryotic growth division process, much less is known about metabolic biosynthetic dynamics during cycle. Here, review recent discoveries made at single-cell population level that show budding yeast (Saccharomyces cerevisiae) metabolism oscillates in synchrony with cycle actively dividing cells, as well independently when halted. In fact, emerging evidence suggests cycle-independent oscillations interact elements machinery via several possible mechanisms. Furthermore, reports indicate different processes exhibit temporally changing activity patterns Thus, resources are drawn from primary dynamic manner, potentially giving rise to oscillations. Finally, highlight work mammalian cells indicating similar might also exist higher eukaryotes.

Language: Английский

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