Oxygenation influences xylose fermentation and gene expression in the yeast genera Spathaspora and Scheffersomyces

Biotechnology for Biofuels and Bioproducts, Journal Year: 2024, Volume and Issue: 17(1)

Published: Feb. 7, 2024

Abstract Background Cost-effective production of biofuels from lignocellulose requires the fermentation d -xylose. Many yeast species within and closely related to genera Spathaspora Scheffersomyces (both order Serinales) natively assimilate ferment xylose. Other consume xylose inefficiently, leading extracellular accumulation xylitol. Xylitol excretion is thought be due different cofactor requirements first two steps metabolism. Xylose reductase (XR) generally uses NADPH reduce xylitol, while xylitol dehydrogenase (XDH) NAD + oxidize xylulose, creating an imbalanced redox pathway. This imbalance particularly consequential in hypoxic or anoxic environments. Results We screened growth xylose-fermenting high moderate aeration identified both ethanol producers producers. Selected were further characterized for their XR XDH preferences by enzyme assays gene expression patterns RNA-Seq. Our data revealed that metabolism more balanced some species, but it strongly affected oxygen levels. Under aeration, most switched accumulation, despite availability ample accept electrons NADH. switch was followed decreases activity genes metabolism, suggesting bottlenecks are not always preferences. Finally, we expressed XYL multiple a strain Saccharomyces cerevisiae . Recombinant S. expressing XYL1 xylosifermentans , which encodes without preference, showed improved anaerobic on as primary carbon source compared stipitis Conclusion Collectively, our do support hypothesis occurs primarily differences between dehydrogenase; instead, plays major role response have also Sc. potential can engineered into improve biofuel production.

Language: Английский

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Yeasts from tropical forests: Biodiversity, ecological interactions, and as sources of bioinnovation

Yeast, Journal Year: 2023, Volume and Issue: 40(11), P. 511 - 539

Published: Nov. 1, 2023

Abstract Tropical rainforests and related biomes are found in Asia, Australia, Africa, Central South America, Mexico, many Pacific Islands. These encompass less than 20% of Earth's terrestrial area, may contain about 50% the planet's biodiversity, endangered regions vulnerable to deforestation. have a great diversity substrates that can be colonized by yeasts. unicellular fungi contribute recycling organic matter, serve as food source for other organisms, or ecological interactions benefit harm plants, animals, fungi. In this review, we summarize most important studies yeast biodiversity carried out these biomes, well new data, discuss ecology genera frequently isolated from tropical forests potential microorganisms bioinnovation. We show forest represent tremendous species. Although studies, using culture‐dependent methods, already been Africa Australasia remain an underexplored novel hope review will encourage researchers study yeasts unexplored habitats.

Language: Английский

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Vanderwaltozyma urihicola sp. nov., a yeast species isolated from rotting wood and beetles in a Brazilian Amazonian rainforest biome

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Journal Year: 2025, Volume and Issue: 75(3)

Published: March 13, 2025

Five yeast isolates belonging to a candidate for novel species were obtained from rotting wood and the gut of passalid beetle larva in site Amazonian rainforest biome Brazil. Sequence analysis Internal Transcribed Spacer (ITS)-5.8S region D1/D2 domains large subunit rRNA gene showed that represent genus Vanderwaltozyma . The closest relative is huisunica These differs due 44 nt substitutions 21 indels sequences ITS region, as well by 15 four domains. A phylogenomic with genomes sequenced this an outgroup other genus. We propose name urihicola sp. nov. (CBS 18107 T , MycoBank MB 856975) accommodate these isolates. homothallic, producing one two ascospores per ascus. habitat V. Brazilian biome.

Language: Английский

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Innovative pathways for ethanol production: Harnessing xylose’s bioenergy potential using Brazilian wild isolated yeasts

Bioresource Technology, Journal Year: 2024, Volume and Issue: 404, P. 130930 - 130930

Published: June 4, 2024

Language: Английский

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Spathaspora marinasilvae sp. nov., a xylose‐fermenting yeast isolated from galleries of passalid beetles and rotting wood in the Amazonian rainforest biome

Yeast, Journal Year: 2024, Volume and Issue: 41(7), P. 437 - 447

Published: June 8, 2024

Abstract Four yeast isolates were obtained from rotting wood and galleries of passalid beetles collected in different sites the Brazilian Amazonian Rainforest Brazil. This produces unconjugated allantoid asci each with a single elongated ascospore curved ends. Sequence analysis internal transcribed spacer‐5.8 S region D1/D2 domains large subunit ribosomal RNA (rRNA) gene showed that represent novel species genus Spathaspora . The is phylogenetically related to subclade containing arborariae suhii Phylogenomic based on 1884 single‐copy orthologs for set whose whole genome sequences are available confirmed represented by strain UFMG‐CM‐Y285 close Sp. name marinasilvae sp. nov. proposed accommodate species. holotype CBS 13467 T (MycoBank 852799). was able accumulate xylitol produce ethanol d ‐xylose, trait biotechnological interest common several

Language: Английский

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Fungal numbers: global needs for a realistic assessment

Fungal Diversity, Journal Year: 2024, Volume and Issue: 128(1), P. 191 - 225

Published: Nov. 7, 2024

Language: Английский

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Sugiyamaella bielyi f. a., sp. nov. and Sugiyamaella amazoniana f. a., sp. nov., two yeast species isolated from passalid beetles and rotting wood in Amazonia

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Journal Year: 2023, Volume and Issue: 73(4)

Published: April 19, 2023

Sixteen yeast isolates representing two novel species of the genus Sugiyamaella were obtained from passalid beetles, their galleries and rotting wood collected in three sites Amazonian Forest Brazil. Sequence analyses ITS-5.8S region D1/D2 domains large subunit rRNA gene showed that first species, described here as amazoniana f. a., sp. nov. (holotype CBS 18112, MycoBank 847461) is phylogenetically related to S. bonitensis with these differing by 37 nucleotide substitutions six gaps sequences. represented nine guts beetles Popilius marginatus, Veturius magdalenae, sinuosus Spasalus aquinoi, a beetle gallery wood. The second bielyi 18148, 847463), most several undescribed species. based on seven V. magdalenae sinuosus, Both appear be associated ecological niches biome.

Language: Английский

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Yueomyces silvicola sp. nov., a novel ascomycetous yeast species unable to utilize ammonium, glutamate, and glutamine as sole nitrogen sources

Yeast, Journal Year: 2023, Volume and Issue: 40(11), P. 540 - 549

Published: Oct. 11, 2023

Five yeast strains isolated from tree bark and rotten wood collected in central southwestern China, together with four Brazilian (three soil rotting an Amazonian rainforest biome one Bromeliad Alagoas state) Costa Rican strain a flower beetle, represent new species closely related Yueomyces sinensis Saccharomycetaceae, as revealed by the 26S ribosomal RNA gene D1/D2 domain internal transcribed spacer region sequence analysis. The name silvicola sp. nov. is proposed for this holotype China General Microbiological Culture Collection Center 2.6469 (= Japan of Microorganisms 34885). exhibits whole-genome average nucleotide identity value 77.8% Y. sinensis. two shared unique physiological characteristics being unable to utilize ammonium majority amino acids, including glutamate glutamine, sole nitrogen sources. Among 20 acids tested, only leucine tyrosine can be utilized species. Genome comparison showed that GAT1, which encodes GATA family protein participating transcriptional activation nitrogen-catabolic genes Saccharomyces cerevisiae, absent However, failure ammonium, glutamate, are generally preferred sources microorganisms, implies more complicated alterations metabolism pathway might occur genus Yueomyces.

Language: Английский

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Spencermartinsiella nicolii sp. nov., a potential opportunistic pathogenic yeast species isolated from rotting wood in Brazil

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Journal Year: 2024, Volume and Issue: 74(9)

Published: Sept. 6, 2024

Nineteen isolates representing a candidate for novel yeast species belonging to the genus Spencermartinsiella were recovered from rotting wood samples collected at different sites in Atlantic Rainforest and Amazonian Forest ecosystems Brazil. Similarity search of nucleotide sequence intergenic spacer (ITS)-5.8S large subunit D1/D2 regions ribosomal gene cluster showed that this is closely related cellulosicola . The differ by four substitutions domain six 31 indels ITS region holotype S. Phylogenomic analysis based on 1474 single-copy orthologues set whose whole genome sequences are available confirmed phylogenetically close low average identity value 83% observed between confirms they distinct. produced asci with hemispherical ascospores. name nicolii sp. nov. proposed. CBS 14238 T MycoBank number MB855027. Interestingly, was identical an uncultured strain causing systemic infection male adult crocodile ( Crocodylus niloticus ). characterization some virulence factors antifungal susceptibility suggest may be opportunistic pathogen animals, including humans; grow 37 °C.

Language: Английский

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Assembly and comparative genome analysis of a Patagonian Aureobasidium pullulans isolate reveals unexpected intraspecific variation

Yeast, Journal Year: 2023, Volume and Issue: 40(5-6), P. 197 - 213

Published: April 28, 2023

Aureobasidium pullulans is a yeast-like fungus with remarkable phenotypic plasticity widely studied for its importance the pharmaceutical and food industries. So far, genomic studies strains from all over world suggest they constitute genetically unstructured population, no association by habitat. However, mechanisms which this genome supports so many permutations are still poorly understood. Recent works have shown of sequencing yeast genomes extreme environments to increase repertoire diversity unconventional yeasts. In study, we present draft A. strain Patagonian hotspot, re-evaluate taxonomic classification based on taxogenomic approaches, annotate high-depth transcriptomic data. Our analysis suggests isolate could be considered novel variant at an early stage speciation process. The discovery divergent in genomically homogeneous group, such as pullulans, can valuable understanding evolution species. identification characterization new variants will not only allow finding unique traits biotechnological importance, but also optimize choice whose phenotypes characterized, providing elements explore questions about adaptation.

Language: Английский

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