bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown
Published: Dec. 21, 2023
Abstract Two endangered Przewalski’s horse stallions were cloned from fibroblast cells cultured and cryopreserved in 1980. These are clones of a male that lived 1975-1998 pedigree analyses identified as genetically valuable for present-day conservation breeding. This is the first time multiple healthy have been produced an species.
Language: Английский
Animals, Journal Year: 2025, Volume and Issue: 15(5), P. 613 - 613
Published: Feb. 20, 2025
Cloning from historically cryopreserved cells offers a potential means to restore lost genetic variation or increase the representation of particular lineages within bottlenecked species, provided such biobanked materials are archived for rescue applications. One species which cloning can provide management benefits is Przewalski's horse, Equus przewalskii. All ~1800 living horses, distributed across ex situ breeding facilities and in reintroduction sites, descended one more five founder established by 12 horses captured wild between 1898 1947. Since 1970s, San Diego Zoo Wildlife Alliance Biodiversity Bank's Frozen Zoo® (Escondido, CA, USA) has tissues 575 individuals spanning many generations. A pedigree analysis subset deceased represented revealed an underrepresented male that lived 1975 1998, Studbook Number 615 (also known as Kuporovitch), who would be high value if cloned. Here, we report two healthy clones were produced this cell line using cross-species somatic nuclear transfer 2020 2023. Their identification was verified standard horse-pedigree genotyping panel, and, clone, whole genome sequencing comparison original donor performed. This first time multiple surviving perinatal period have been endangered species.
Language: Английский
Citations
1
Development, Journal Year: 2024, Volume and Issue: 151(20)
Published: Oct. 9, 2024
ABSTRACT Wildlife biodiversity is essential for healthy, resilient and sustainable ecosystems. For biologists, this diversity also represents a treasure trove of genetic, molecular developmental mechanisms that deepen our understanding the origins rules life. However, rapid decline in reported recently foreshadows potentially catastrophic collapse many important ecosystems associated irreversible loss forms life on planet. Immediate action by conservationists all stripes required to avert disaster. In Spotlight, we draw together insights proposals discussed at recent workshop hosted Revive & Restore, which gathered experts discuss how stem cell technologies can support traditional conservation techniques help protect animal biodiversity. We reprogramming, vitro gametogenesis, disease modelling embryo modelling, highlight prospects leveraging beyond mammalian species.
Language: Английский
Citations
6
Molecular Ecology Resources, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 10, 2025
ABSTRACT Illegal wildlife trade is a growing problem internationally. Poaching of animals not only leads to the extinction populations and species but also has serious consequences for ecosystems economies. This study introduces molecular marker system that authorities can use detect substantiate trafficking. SNPSTR markers combine short tandem repeats with single nucleotide polymorphisms within an amplicon increase discriminatory power. Within FOGS (Forensic Genetics Species Protection) project, we have established sets 74 vertebrate species. On average, each set consists 19 82 SNPs per set. More than 1300 over 300 STR were identified. Also, through its biobanking pipeline, project enabled cryopreservation somatic cells from 91 as well viable tissues later cell initiation further 109 species, providing future strategies ex situ conservation. In addition, many more fixed DNA samples endangered biobanked. Therefore, was interdisciplinary study, combining forensics conservation tools. The culture information are accessible database ( https://fogs‐portal.de/data ) open scientists, researchers, breeders worldwide protect illegal trade.
Language: Английский
Citations
0
Theriogenology Wild, Journal Year: 2025, Volume and Issue: unknown, P. 100119 - 100119
Published: Jan. 1, 2025
Language: Английский
Citations
0
Gene Reports, Journal Year: 2025, Volume and Issue: unknown, P. 102166 - 102166
Published: Feb. 1, 2025
Language: Английский
Citations
0
Biopreservation and Biobanking, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 12, 2025
Nearly one-third of flora, fauna, and funga species on Earth are threatened with extinction. In response, the prevalence repositories-often called "biobanks" or "genome resource banks"-for storing biological materials from has become more widespread. This research examined trends for (1) terminology, (2) taxa representation, (3) global distribution, (4) operational approach biobanks versus genome banks relating to zoos wildlife. Our literature search results indicate that although banking began earlier in 1990s, biobanking seen a surge publications over 3.5× since 2020. Genome bank articles were highly focused mammals (68%), while multi-taxonomic overviews less-studied taxa. parameters found largest number wildlife Europe (18) lowest South America (2), though likely impacted by being completed English. Additionally, only 28% (7/25) biodiversity hotspots contain biobank based our methodology. While not all efforts published reported, these findings suggest "biobank" will be widely used term future, is needed non-mammalian taxa, there geographical gaps biobanks, conservation programs should focus biospecimens wide set individuals develop assisted reproductive technologies concomitantly goal maintaining healthy, sustainable populations long term.
Language: Английский
Citations
0
Frontiers in Marine Science, Journal Year: 2025, Volume and Issue: 11
Published: Feb. 17, 2025
Biorepositories, or biobanks, are vital to marine science. Their collections safeguard biological knowledge, enable follow-up studies and reproducibility confirmations, help extend ecological baselines. Biorepository networks data portals aggregate catalogs facilitate open material exchange. Such integrations enrich contextual support holistic ecosystem-based research management. In the Arctic, where researchers face vast scales, rapidly changing ecosystems, limited resampling opportunities, biobanking builds capacities. However, polar biodiversity remains underrepresented in collections. Heterogeneous methodologies documentation practices hinder integrations. And science faces high institutional cultural barriers. Here, we explore potential of amplify impact individual studies. We address gaps standardization vouchering suggest improvements funding publishing models incentivize collaboration. bring together calls for advancements from diverse perspectives provide examples expeditions, databases, specimen collections, standards. The general analysis is illustrated with two case studies, showcasing range field: inclusion citizen observations cetacean monitoring, preservation specimens environmental microbiome former, strategies harmonizing collection global databases. latter, propose cooperative field intact living (complex microbial community) cryopreservation. Our perspective frames as a strategy, essential accelerating under current climate change-related pressures. advocate international investment precautionary approach academic conservation stewardship Arctic heritage.
Language: Английский
Citations
0
Biopreservation and Biobanking, Journal Year: 2025, Volume and Issue: unknown
Published: March 27, 2025
Sampling skin fragments has been an important strategy for genetic studies and ex situ conservation, aiding in the preservation of diversity Neotropical deer other wild species. From moment collection field, transport media must ensure tissue viability by providing necessary nutrients until laboratory processing culture or cryopreservation. This study aimed to evaluate effects temperature storage duration on cell growth using two types media: Dulbecco's modified Eagle medium (DMEM) supplemented with 15% fetal bovine serum 0.9% physiological saline solution. Skin were collected from inguinal region five captive gray-brocket (Subulo gouazoubira) divided into small samples, which randomly assigned each medium. The samples stored at 5°C 24°C 24 72 hours, followed cryopreservation thawing assess histomorphology, apoptosis (TUNEL test), growth, (Trypan blue MTT assay), mitotic index. results showed that solution is as efficient DMEM maintaining viability, 80% viable cells observed no significant difference after storing different (p > 0.05). Cell morphology did not change response media, temperature, duration. We recovered metaphases all conditions, a similar index those presented biopsies. These feasibility during hours guaranteeing reproductive biotechnologies. may contribute sampling places where displacement large equipment limited, allowing establishment simplified protocols step accessing material individuals inhabiting isolated localities.
Language: Английский
Citations
0
In Vitro Cellular & Developmental Biology - Animal, Journal Year: 2025, Volume and Issue: unknown
Published: April 23, 2025
Language: Английский
Citations
0
Animals, Journal Year: 2024, Volume and Issue: 14(5), P. 686 - 686
Published: Feb. 22, 2024
The vulnerable status of the Amazon manatee, Trichechus inunguis, indicates need to seek measures guarantee its conservation. In this context, cultivation cells in vitro is a strategy that should at least preservation their genetic material. Thus, we established for first time primary culture Amazonian manatee fibroblasts (TINsf) from skin biopsy young male. Karyotypic analysis 3rd, 7th, and 12th passages confirmed taxonomic identity species T. inunguis (2n = 56/NF 92) indicated presents genomic stability. Gene protein expression vimentin 13th passage show predominant presence TINsf. To test cell line’s responsiveness materials demonstrate possible application culture, it was exposed andiroba seed oil (ASO), viability proliferative capacity were evaluated. ASO demonstrated toxic effects highest concentrations longest exposure times tested, reproducing results observed human cultures, indicating applicability TINsf toxicological biotechnological studies. After cryopreservation, line maintained potential, establishment new available future
Language: Английский
Citations
3