Insights into Grapevine Defence Response Against Fungal and Oomycete Diseases Towards a Sustainable Plant Breeding DOI
Mariana Patanita, Maria do Rosário Félix, Joana A. Ribeiro

et al.

Published: Jan. 1, 2023

Downy mildew, powdery grey mould, black rot, and grapevine trunk diseases are among the most important fungal or oomycete affecting grapevine, with a high economic impact in all growing areas. In recent decades, extensive efforts have been made to reduce use of agrochemicals viticulture, turning management these major challenge. Given urgent need identify develop new sustainable effective protection strategies, better understanding plant defence mechanisms is essential more tolerant plants provide valuable insights for disease management. Although advances molecular technologies allowed identification several resistance genes, little known about complexity involved grapevine-pathogen interactions. This chapter aims bring together up-to-date findings on regulation against by enumerating promising candidate genes that offer broad possibilities inducing specific pathogens. We also focus functional genomics as tool study immunity different pathogens, particular emphasis emerging will undoubtedly contribute breeding.

Language: Английский

DNA-free genome editing in grapevine using CRISPR/Cas9 ribonucleoprotein complexes followed by protoplast regeneration DOI Creative Commons

Samaneh Najafi,

Edoardo Bertini, Erica D’Incà

et al.

Horticulture Research, Journal Year: 2022, Volume and Issue: 10(1)

Published: Oct. 26, 2022

CRISPR/Cas9 genome editing technology can overcome many limitations of traditional breeding, offering enormous potential for crop improvement and food production. Although the direct delivery Cas9-single guide RNA (sgRNA) ribonucleoprotein (RNP) complexes to grapevine (Vitis vinifera) protoplasts has been shown before, regeneration edited into whole plants not reported. Here, we describe an efficient approach obtain transgene-free by transfection subsequent isolated from embryogenic callus. As proof concept, a single-copy green fluorescent protein reporter gene (GFP) in cultivar Thompson Seedless was targeted knocked out RNPs protoplasts. activity, guided two independent sgRNAs, confirmed loss GFP fluorescence. The GFP- monitored throughout development, confirming that were comparable morphology growth habit wild-type controls. We report first highly protocol DNA-free preassembled Cas9-sgRNA RNP protoplasts, helping address regulatory concerns related genetically modified plants. This could encourage application genetic other woody

Language: Английский

Citations

43
European grapevine moth, Lobesia botrana Part II: Prevention and management DOI
Giovanni Benelli, Andrea Lucchi, Gianfranco Anfora

et al.

Entomologia Generalis, Journal Year: 2023, Volume and Issue: 43(2), P. 281 - 304

Published: April 28, 2023

Lobesia botrana (Denis & Schiffermüller) (Lepidoptera: Tortricidae), commonly known as the European grapevine moth (EGVM), is a primary pest of vineyards.This article provides an updated review its monitoring, modelling, and management tools.EGVM strategies analysed here include insecticide-based control, insecticide resistance, side-effects (particularly those caused by exposure to sublethal doses pesticides), cultural sterile insect technique, pheromone-mediated control (with special reference pheromone-based mating disruption), biological area-wide programs.Lastly, we outline significant challenges for future EGVM research sustainable implementation.

Language: Английский

Citations

25
Simultaneous editing of two DMR6 genes in grapevine results in reduced susceptibility to downy mildew DOI Creative Commons
Lisa Giacomelli,

Tieme Zeilmaker,

Oscar Giovannini

et al.

Frontiers in Plant Science, Journal Year: 2023, Volume and Issue: 14

Published: Aug. 21, 2023

The reduction of pesticide treatments is paramount importance for the sustainability viticulture, and it can be achieved through a combination strategies, including cultivation vines ( Vitis vinifera ) that are resistant or tolerant to diseases such as downy mildew (DM). In many crops, knock-out Downy Mildew Resistant 6 DMR6 proved successful in controlling DM-resistance, but effect mutations genes not yet known grapevine. Today, gene editing serves crop improvement with small specific while maintaining genetic background commercially important clones. Moreover, recent technological advances allowed produce non-transgenic grapevine clones by regeneration protoplasts edited CRISPR/Cas9 ribonucleoprotein. This approach may revolutionize production new varieties clones, requires knowledge about targets impact on plant phenotype fitness different cultivars. this work we generated single double mutants susceptibility (S) using CRISPR/Cas9, showed only combined VviDMR6-1 VviDMR6-2 effective reducing DM two table-grape cultivars increasing levels endogenous salicylic acid. Therefore, both necessary control real-world agricultural settings, which could potentially lead unwanted phenotypes. Additional research, trials conducted experimental vineyards, required gain deeper understanding -based resistance.

Language: Английский

Citations

18
The pathogenicity of Plasmopara viticola: a review of evolutionary dynamics, infection strategies and effector molecules DOI Creative Commons
Catarina Gouveia, Rita B. Santos, Catarina Paiva-Silva

et al.

BMC Plant Biology, Journal Year: 2024, Volume and Issue: 24(1)

Published: April 24, 2024

Abstract Oomycetes are filamentous organisms that resemble fungi in terms of morphology and life cycle, primarily due to convergent evolution. The success pathogenic oomycetes lies their ability adapt overcome host resistance, occasionally transitioning new hosts. During plant infection, these secrete effector proteins other compounds during as a molecular arsenal contributes success. Genomic sequencing, transcriptomic analysis, proteomic studies have revealed highly diverse repertoires among different oomycete pathogens, highlighting adaptability evolution potential. obligate biotrophic Plasmopara viticola affects grapevine plants ( Vitis vinifera L.) causing the downy mildew disease, with significant economic impact. This disease is devastating Europe, leading substantial production losses. Even though well-known pathogen, date there scarce reviews summarising pathogenicity, virulence, genetics mechanisms interaction grapevine. review aims explore current knowledge infection strategy, lifecycle, molecules, pathogenicity . recent sequencing genome has provided insights into understanding strategies employed by this pathogen. Additionally, we will highlight contributions omics technologies unravelling ongoing oomycete, including first in-plant proteome analysis

Language: Английский

Citations

7
Genome editing of a recalcitrant wine grape genotype by lipofectamine‐mediated delivery of CRISPR/Cas9 ribonucleoproteins to protoplasts DOI Creative Commons
Giorgio Gambino, Floriana Nuzzo, Amedeo Moine

et al.

The Plant Journal, Journal Year: 2024, Volume and Issue: 119(1), P. 404 - 412

Published: April 22, 2024

SUMMARY The main bottleneck in the application of biotechnological breeding methods to woody species is due vitro regeneration recalcitrance shown by several genotypes. On other side, species, especially grapevine ( Vitis vinifera L.), use most pesticides and expensive inputs agriculture, making development efficient approaches genetic improvement absolutely urgent. Genome editing an extremely promising technique particularly for wine grape genotypes, as it allows modify desired gene a single step, preserving all quality traits selected appreciated elite varieties. A genome protocol production transgene‐free plants, exploiting lipofectamine‐mediated direct delivery CRISPR–Cas9 ribonucleoproteins (RNPs) target phytoene desaturase gene, reported. We focused on Nebbiolo V. ), recalcitrant genotype used produce outstanding wines, such Barolo Barbaresco. PEG‐mediated method available literature employed highly embryogenic genotypes did not allow proper embryo Nebbiolo. Lipofectamines, contrary, have negative impact protoplast viability plant regeneration, leading obtainment fully developed edited plants after about 5 months from transfection. Our work represents one first examples lipofectamine delivering reagents protoplasts. important result achieved could be extended varieties species.

Language: Английский

Citations

5
The Role of Italy in the Use of Advanced Plant Genomic Techniques on Fruit Trees: State of the Art and Future Perspectives DOI Open Access
Luca Nerva, Lorenza Dalla Costa, Angelo Ciacciulli

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(2), P. 977 - 977

Published: Jan. 4, 2023

Climate change is deeply impacting the food chain production, lowering quality and yield. In this context, international scientific community has dedicated many efforts to enhancing resilience sustainability in agriculture. Italy among main European producers of several fruit trees; therefore, national research centers universities undertook initiatives maintain specificity ‘Made Italy’ label. Despite their importance, crops are suffering from difficulties associated with conventional breeding approaches, especially terms financial commitment, land resources availability, long generation times. The ‘new genomic techniques’ (NGTs), renamed as ‘technologies for assisted evolution’ (TEAs), reduce time required obtain genetically improved cultivars while precisely targeting specific DNA sequences. This review aims illustrate role Italian use NGTs, a focus on Citrus, grapevine, apple, pear, chestnut, strawberry, peach, kiwifruit. For each crop, key genes traits which working, well technological improvements advancements regeneration local varieties, presented. Lastly, placed legal aspects contexts.

Language: Английский

Citations

12
A high-throughput ResNet CNN approach for automated grapevine leaf hair quantification DOI Creative Commons
Nagarjun Malagol,

Tanuj Rao,

Anna Werner

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 10, 2025

Abstract The hairiness of the leaves is an essential morphological feature within genus Vitis that can serve as a physical barrier. A high leaf hair density present on abaxial surface grapevine influences their wettability by repelling forces, thus preventing pathogen attack such downy mildew and anthracnose. Moreover, hairs favorable habitat may considerably affect abundance biological control agents. unavailability accurate efficient objective tools for quantifying makes study intricate challenging. Therefore, validated high-throughput phenotyping tool was developed established in order to detect quantify using images single discs convolution neural networks (CNN). We trained modified ResNet CNNs with minimalistic number efficiently classify area covered hairs. This approach achieved overall model prediction accuracy 95.41%. As final validation, 10,120 input from segregating F1 biparental population were used evaluate algorithm performance. CNN-based phenotypic results compared ground truth data received two experts revealed strong correlation R values 0.98 0.92 root-mean-square error 8.20% 14.18%, indicating performance consistent expert evaluations outperforms traditional manual rating. Additional validation between vs. non-expert six varieties showed non-experts contributed over- underestimation trait, absolute 0% 30% -5% -60%, respectively. Furthermore, panel 16 novice evaluators produced significant bias set varieties. Our provide clear evidence need hairiness.

Language: Английский

Citations

0
Biocontrol Potential of Endophytic Fungi Against Postharvest Grape Pathogens DOI
Amr H. Hashem, Amer M. Abdelaziz, Mohamed S. Attia

et al.

Published: Jan. 1, 2025

Language: Английский

Citations

0
Chitinases: Key players in plant defense mechanisms against fungal pathogens DOI

Gaytri Mahajan,

Nivedita Sharma, Manpreet Kaur

et al.

Physiological and Molecular Plant Pathology, Journal Year: 2025, Volume and Issue: unknown, P. 102664 - 102664

Published: March 1, 2025

Language: Английский

Citations

0
Wild grapes of Armenia: unexplored source of genetic diversity and disease resistance DOI Creative Commons
K. Margaryan, Reinhard Töpfer,

Boris Gasparyan

et al.

Frontiers in Plant Science, Journal Year: 2023, Volume and Issue: 14

Published: Dec. 8, 2023

The present study is the first in-depth research evaluating genetic diversity and potential resistance of Armenian wild grapes utilizing DNA-based markers to understand signature this unexplored germplasm. In proposed research, five geographical regions with known viticultural history were explored. A total 148 unique genotypes collected included in 48 individuals previously as seed. 24 nSSR utilized establish a fingerprint database infer information on population structure. Three linked Ren1 locus analyzed identify against powdery mildew. According molecular fingerprinting data, V. sylvestris gene pool conserves high diversity, displaying 292 different alleles 12.167 allele per loci. clustering analyses parameters supported eight groups 5.6% admixed proportion. polymorphism at revealed that 28 carried three R-alleles 34 two associated PM among 107 individuals. This richness represents an immense reservoir under-explored breeding potential. Therefore, continued survey efforts are crucial for conservation, sustainable management, utilization grape resources face emerging challenges viticulture.

Language: Английский

Citations

9