mSphere, Journal Year: 2024, Volume and Issue: unknown
Published: Dec. 9, 2024
It is unclear whether human progression to active tuberculosis disease (TB) risk signatures are viable endpoint criteria for evaluations of treatments in development. TB the deadliest infectious globally and more efficacious vaccines needed reduce this mortality. However, immune correlates protection either preventing infection with
Language: Английский
Respiratory Research, Journal Year: 2025, Volume and Issue: 26(1)
Published: March 5, 2025
This study aims to explore the role of autophagy-associated genes (ATG) and their epigenetic markers in progression mycobacterium tuberculosis (M. tb) infection, test effects de-methylation agents on macrophage functions against TB. ATG expressions gene promoter DNA methylation levels blood immune cells were measured 60 patients with active pulmonary TB disease, 31 subjects latent infection (LTBI), 15 non-infected healthy (NIHS). An vitro monocytic THP-1 cell culture model under M. tb-specific antigen stimuli was applied. LC3B protein expression M1/M2a monocyte, ATG5 M2a, mean region peripheral mononuclear all increased versus either LTBI or NIHS group. The negatively correlated its expressions. discrimination disease from optimally captured by prediction scores, which combined (+) percentage level, male gender, body mass index. both M2a neutrophil decreased after 6-month anti-TB therapy, but hypermethylated persisted. In 5-Aza-2'-deoxycytidine treatment improved bactericidal, apoptosis phagocytosis through augmenting autophagy flux via mechanisms other than demethylation cells. Increased hypermethylation may serve as biomarkers for tb while use agent be a potential approach host-directed immunotherapy disease.
Language: Английский
Citations
0
The Lancet Microbe, Journal Year: 2025, Volume and Issue: unknown, P. 101134 - 101134
Published: April 1, 2025
Language: Английский
Citations
0
Frontiers in Public Health, Journal Year: 2025, Volume and Issue: 13
Published: May 23, 2025
Introduction This study aimed to investigate the prevalence of resistance first-line anti-tuberculosis (TB) drugs and molecular mechanisms underlying mutations in patients with culture-positive Mycobacterium tuberculosis complex (MTBC). The findings provide a data basis for developing more precise regionally tailored anti-TB treatment regimens. Methods From 2015 2022, total 3,605 strains isolated from 10 designated TB medical institutions main urban county/township areas Luoyang City, China, were confirmed as MTBC members through polymerase chain reaction (PCR) targeting specific insertion sequence IS6110. Drug susceptibility testing using proportional method was performed analyze patterns drugs, namely, isoniazid (INH), rifampin (RFP), streptomycin (SM), ethambutol (EMB). Molecular drug conducted on resistant multicolor melting curve analysis (MMCA) determine mutation associated phenotypic resistance. Results Among cases, 79.5% (2,866 cases) male, 64.9% (2,341 resided county township areas, 64.8% (2,336 younger than 60 years. rates highest lowest, SM (16.5%), INH (15.7%), RFP (9.9%), EMB (6.4%). overall significantly higher areas. During period, proportion mono-resistance (MR-TB), multidrug-resistant (MDR-TB) polydrug-resistant (PDR-TB) decreased by 59.2% (12.9–5.3%), 40.3% (12.4–7.4%), 68.3% (6.9–2.2%), respectively. predominant MDR-TB PDR-TB MDR4 (INH + RIF SM) PDR2 SM). significant observed rpsL 43 (66.2%, 344 cases), katG 315 (70.6%, 361 rpoB 529-533 (54.0%, 183 embB 306 (56.5%, 108 cases). Resistance cases frequently involved combinations hotspot but not strictly confined these sites. Conclusion Tuberculosis have declined over time, distinct regional variations patterns. Significant responsible predominantly involve common mutations, they are limited these.
Language: Английский
Citations
0
Biochemical Genetics, Journal Year: 2024, Volume and Issue: unknown
Published: May 30, 2024
Language: Английский
Citations
3
Medicina, Journal Year: 2024, Volume and Issue: 60(12), P. 2071 - 2071
Published: Dec. 16, 2024
Background and Objectives: Coinfection with SARS-CoV-2 extrapulmonary tuberculosis (extraPTB) presents unique clinical challenges due to dual inflammatory responses potential differences in patient profiles compared those infection alone. This study uniquely contributes the underexplored interaction between extraPTB SARS-CoV-2, focusing on systemic inflammation as a critical determinant of outcomes. Materials Methods: retrospective, cross-sectional included 123 patients aged 19–91 years, hospitalized at Victor Babeș Hospital Timișoara from March 2020 2022. We 23 coinfected 100 age-matched SARS-CoV-2-only patients. Clinical records were examined for demographic, clinical, laboratory data. Results: The group was younger, 65% under 40 presented significantly higher IL-6, PCT, transaminase levels. Coexisting COPD type 2 diabetes independent predictors coinfection. A SpO2 diagnosis positively associated coinfection likelihood (OR = 5.37), while CT scores indicated less pulmonary involvement Non-fatal outcomes more frequent (95.7% sensitivity), only one had fatal outcome versus 17 group. Low elevated IL-6 significant mortality, severe symptoms tripling fatality odds. Conclusions: is younger age, heightened inflammation, longer hospital stays but does not increase mortality risk These findings underscore importance monitoring markers developing tailored management strategies improve long-term care patients, especially resource-limited settings.
Language: Английский
Citations
1
Archivos de Bronconeumología, Journal Year: 2024, Volume and Issue: 60(5), P. 312 - 315
Published: Feb. 13, 2024
Language: Английский
Citations
0
Turkish Journal of Clinics and Laboratory, Journal Year: 2024, Volume and Issue: 15(2), P. 169 - 175
Published: June 25, 2024
Aim: We aimed to determine the antituberculosis drug susceptibility status in Mycobacterium tuberculosis complex isolates, considering that current resistance rates will be an important indicator of prevalence primary future. Material and Methods: All cases whose culture sample was taken with clinical suspicion at Samsun Training Research Hospital period between January 2018 December 2023 who had a positive result least one Acid-fast stain (AFS) methods were included study. Results: The average age patients 54.5±18.5 (range: 17-93) 398 (74.1%) male. In study, 77.3% samples sputum 15.8% bronchoalveolar lavage. 474 (88.3%) isolates M. complex. 49% AFS method, 96.5% Mycobacteria Growth Indicator Tube (MGIT), 84.5% Löwenstein-Jensen (LJ) medium. 10.6%, 2.8%, 1.1% 7.0% for isoniazid, rifampicin, ethambutol streptomycin, respectively. resistant rifampicin also isoniazid. rate multidrug-resistant found 2.8%. single 14.0%. It determined before pandemic significantly higher than during period. Conclusion: isoniazid streptomycin around 10% general anti-tuberculosis drugs decreased
Language: Английский
Citations
0
BMC Infectious Diseases, Journal Year: 2024, Volume and Issue: 24(1)
Published: Oct. 30, 2024
China is ranked third globally in terms of burden and has a moderately high to prevalence tuberculosis (TB). This study meticulously investigated the notification rates TB assessed epidemic from 2000 2021. The aim was provide robust supporting data that crucial for enhancing prevention control strategies.
Language: Английский
Citations
0
mSphere, Journal Year: 2024, Volume and Issue: unknown
Published: Dec. 9, 2024
It is unclear whether human progression to active tuberculosis disease (TB) risk signatures are viable endpoint criteria for evaluations of treatments in development. TB the deadliest infectious globally and more efficacious vaccines needed reduce this mortality. However, immune correlates protection either preventing infection with
Language: Английский
Citations
0