Bioassay-Guided Isolation of Piplartine from Piper purusanum Yunck (Piperaceae) and Evaluation of Its Toxicity Against Aedes aegypti Linnaeus, 1762, Anopheles darlingi Root, 1926 (Culicidae), and Non-Target Animals DOI Creative Commons
André Correa de Oliveira, Felipe M. A. da Silva,

Ingrity Suelen de Sá

et al.

Plants, Journal Year: 2025, Volume and Issue: 14(5), P. 774 - 774

Published: March 3, 2025

Aedes aegypti and Anopheles darlingi are the primary vectors of dengue malaria in Brazil. Natural products currently regarded as promising alternatives for their control, offering environmentally friendly solutions larval management due to low toxicity non-target organisms. Thus, Piplartine, isolated first time from Piper purusanum, exhibited larvicidal activity against Ae. An. (LC50 14.56 26.44 μg/mL), occasioned by overproduction reactive oxygen nitrogen species (66.67 ± 7% 86.33 6%). Furthermore, piplartine enhanced key detoxifying enzymes, including catalase (87.00 9 94.67 μmol H2O2 consumed per minute mg protein), glutathione S-transferase (76.00 1 134.00 μmol/min/mg), mixed-function oxidase (26.67 5 55.00 nmol cti mg⁻¹ α-esterase, β-esterase (27.67 7 46.33 protein). In contrast, inhibited acetylcholinesterase (43.33 48.00 2 μmol/min/mg) compared negative control DMSO (87.33 146.30 3 μmol/min/mg). It is important highlight that showed no lethal effects on aquatic insects, with 100% survival observed at a concentration 264.4 μg/mL. α-cypermethrin demonstrated acute rapid organisms, only 9.1% survival. These findings larvicide selective environmental impact, suitable integrated strategies.

Language: Английский

Bioassay-Guided Isolation of Piplartine from Piper purusanum Yunck (Piperaceae) and Evaluation of Its Toxicity Against Aedes aegypti Linnaeus, 1762, Anopheles darlingi Root, 1926 (Culicidae), and Non-Target Animals DOI Creative Commons
André Correa de Oliveira, Felipe M. A. da Silva,

Ingrity Suelen de Sá

et al.

Plants, Journal Year: 2025, Volume and Issue: 14(5), P. 774 - 774

Published: March 3, 2025

Aedes aegypti and Anopheles darlingi are the primary vectors of dengue malaria in Brazil. Natural products currently regarded as promising alternatives for their control, offering environmentally friendly solutions larval management due to low toxicity non-target organisms. Thus, Piplartine, isolated first time from Piper purusanum, exhibited larvicidal activity against Ae. An. (LC50 14.56 26.44 μg/mL), occasioned by overproduction reactive oxygen nitrogen species (66.67 ± 7% 86.33 6%). Furthermore, piplartine enhanced key detoxifying enzymes, including catalase (87.00 9 94.67 μmol H2O2 consumed per minute mg protein), glutathione S-transferase (76.00 1 134.00 μmol/min/mg), mixed-function oxidase (26.67 5 55.00 nmol cti mg⁻¹ α-esterase, β-esterase (27.67 7 46.33 protein). In contrast, inhibited acetylcholinesterase (43.33 48.00 2 μmol/min/mg) compared negative control DMSO (87.33 146.30 3 μmol/min/mg). It is important highlight that showed no lethal effects on aquatic insects, with 100% survival observed at a concentration 264.4 μg/mL. α-cypermethrin demonstrated acute rapid organisms, only 9.1% survival. These findings larvicide selective environmental impact, suitable integrated strategies.

Language: Английский

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