Biosynthesized sulfur nanoparticles: a novel strategy to enhance antioxidant secondary metabolites in Lotus arabicus L. callus cultures DOI Creative Commons
Sherien E. Sobhy, Asmaa M. Khalifa, E. S. E. Hafez

et al.

BMC Plant Biology, Journal Year: 2025, Volume and Issue: 25(1)

Published: May 7, 2025

Abstract Background Secondary metabolites are distinct compounds with significant medicinal value, yet their production and chemical synthesis present considerable challenges. This necessitates the development of innovative strategies to improve yield. study investigated potential biosynthesized sulfur nanoparticles (SNPs) as an eco-friendly elicitor enhance antioxidant secondary in Lotus arabicus L. callus cultures. Results After seven weeks, induced calli L were transferred MS media supplemented SNPs at different concentrations (0, 25, 50, 100, 200 mg/l). The results indicated that (100 mg/l) significantly higher profiles for biomass metabolite compared control treatments. Enzyme activities related biosynthesis, specifically phenylalanine ammonia lyase (PAL) polyphenol oxidase (PPO) enhanced a dose-dependent manner, greatest increases observed 100 mg/l SNPs. also modulated oxidative stress markers (MDA H 2 O ), generally improving growth conditions by reducing stress, except highest concentration mg/l. Additionally, application markedly upregulated expression levels six crucial genes biosynthesis pathway ( chalcone synthase CHS), PAL), flavonol FLS), isomerase CHI) , hydroxycinnamoyl CoA quinate transferase (HQT) deoxyxylulose phosphate reductoisomerase DXR )). Quantitative HPLC profiling 16 phenolic flavonoid revealed supplementation resulted noticeable boots majority measured SNP supplementation. Conclusion Overall, culture positively influenced molecular physiological levels, increasing its use.

Language: Английский

Biosynthesized sulfur nanoparticles: a novel strategy to enhance antioxidant secondary metabolites in Lotus arabicus L. callus cultures DOI Creative Commons
Sherien E. Sobhy, Asmaa M. Khalifa, E. S. E. Hafez

et al.

BMC Plant Biology, Journal Year: 2025, Volume and Issue: 25(1)

Published: May 7, 2025

Abstract Background Secondary metabolites are distinct compounds with significant medicinal value, yet their production and chemical synthesis present considerable challenges. This necessitates the development of innovative strategies to improve yield. study investigated potential biosynthesized sulfur nanoparticles (SNPs) as an eco-friendly elicitor enhance antioxidant secondary in Lotus arabicus L. callus cultures. Results After seven weeks, induced calli L were transferred MS media supplemented SNPs at different concentrations (0, 25, 50, 100, 200 mg/l). The results indicated that (100 mg/l) significantly higher profiles for biomass metabolite compared control treatments. Enzyme activities related biosynthesis, specifically phenylalanine ammonia lyase (PAL) polyphenol oxidase (PPO) enhanced a dose-dependent manner, greatest increases observed 100 mg/l SNPs. also modulated oxidative stress markers (MDA H 2 O ), generally improving growth conditions by reducing stress, except highest concentration mg/l. Additionally, application markedly upregulated expression levels six crucial genes biosynthesis pathway ( chalcone synthase CHS), PAL), flavonol FLS), isomerase CHI) , hydroxycinnamoyl CoA quinate transferase (HQT) deoxyxylulose phosphate reductoisomerase DXR )). Quantitative HPLC profiling 16 phenolic flavonoid revealed supplementation resulted noticeable boots majority measured SNP supplementation. Conclusion Overall, culture positively influenced molecular physiological levels, increasing its use.

Language: Английский

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