Human heart assembloids with autologous tissue-resident macrophages recreate physiological immuno-cardiac interactions DOI Open Access
Colin O’Hern,

Sammantha Caywood,

Shakhlo Aminova

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 15, 2024

Interactions between the developing heart and embryonic immune system are essential for proper cardiac development maintaining homeostasis, with disruptions linked to various diseases. While human pluripotent stem cell (hPSC)-derived organoids valuable models studying organ function, they often lack critical tissue-resident cells. Here, we introduce an advanced assembloid model, termed hHMA (human heart-macrophage assembloid), which fully integrates autologous tissue- resident macrophages (MPs) pre-existing (hHOs). Through multi-omic analyses, confirmed that these MPs phenotypically similar remain viable in assembloids over time. The inclusion of significantly impacts development, influencing cellular composition, boosting communication, remodeling extracellular matrix, promoting ventricular morphogenesis, enhancing sarcomeric maturation. Our findings indicate contribute homeostasis via efferocytosis, integrate into cardiomyocyte electrical system, support catabolic metabolism. To demonstrate versatility this developed a platform study arrhythmias by chronic exposure pro-inflammatory factors arrhythmogenesis clinical settings, successfully replicating key features inflammasome-mediated atrial fibrillation. Overall, work introduces robust examining role cells disease mechanisms, drug discovery, bridging gap

Language: Английский

Exploring therapy for tooth eruption disturbances from the perspective of ECM-macrophage DOI

Han Qin,

Jun Cai

Medical Hypotheses, Journal Year: 2025, Volume and Issue: unknown, P. 111662 - 111662

Published: May 1, 2025

Language: Английский

Citations

0
Human heart assembloids with autologous tissue-resident macrophages recreate physiological immuno-cardiac interactions DOI Open Access
Colin O’Hern,

Sammantha Caywood,

Shakhlo Aminova

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 15, 2024

Interactions between the developing heart and embryonic immune system are essential for proper cardiac development maintaining homeostasis, with disruptions linked to various diseases. While human pluripotent stem cell (hPSC)-derived organoids valuable models studying organ function, they often lack critical tissue-resident cells. Here, we introduce an advanced assembloid model, termed hHMA (human heart-macrophage assembloid), which fully integrates autologous tissue- resident macrophages (MPs) pre-existing (hHOs). Through multi-omic analyses, confirmed that these MPs phenotypically similar remain viable in assembloids over time. The inclusion of significantly impacts development, influencing cellular composition, boosting communication, remodeling extracellular matrix, promoting ventricular morphogenesis, enhancing sarcomeric maturation. Our findings indicate contribute homeostasis via efferocytosis, integrate into cardiomyocyte electrical system, support catabolic metabolism. To demonstrate versatility this developed a platform study arrhythmias by chronic exposure pro-inflammatory factors arrhythmogenesis clinical settings, successfully replicating key features inflammasome-mediated atrial fibrillation. Overall, work introduces robust examining role cells disease mechanisms, drug discovery, bridging gap

Language: Английский

Citations

3