The Effect of Temperature on Gonadal Sex Differentiation of Yesso Scallop Patinopecten yessoensis DOI Creative Commons

Tian Liu,

Ruojiao Li,

Liangjie Liu

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2022, Volume and Issue: 9

Published: Jan. 31, 2022

Many marine organisms are generally poikilotherms, making seawater temperature one of the most important environmental factors affecting gonadal sex differentiation. Mollusca is second-largest animal phylum with diverse reproductive systems, but studies on impact differentiation limited to a few sequential hermaphrodites. By combining morphological and molecular analyses, we investigated effect commercially gonochoristic scallop Patinopecten yessoensis in field under laboratory conditions. Based relative expression FoxL2 Dmrt1L gonads 6- 12 month-old scallops, found scallops start differentiate at 7 months old September when was 21°C. To eliminate other than differentiation, compared development juvenile different temperatures (21, 16 11°C) After 50 days treatment, 11°C group contain more germ cell types, have higher rates 21°C group. But no obvious bias observed. These results suggest that high (21°C) inhibits whereas low (11°C) accelerates by 2 for this cold-water species. It also supports P. rather protandrous hermaphroditic. Our study addresses first time an influence associated genetic controls will facilitate better understanding how affect especially less studied molluscs.

Language: Английский

Single cell genomics as a transformative approach for aquaculture research and innovation DOI Creative Commons
Rose Ruiz Daniels, Richard S. Taylor, Diego Robledo

et al.

Reviews in Aquaculture, Journal Year: 2023, Volume and Issue: 15(4), P. 1618 - 1637

Published: March 7, 2023

Abstract Single cell genomics encompasses a suite of rapidly maturing technologies that measure the molecular profiles individual cells within target samples. These approaches provide large up‐step in biological information compared to long‐established ‘bulk’ methods profile average all sample, and have led transformative advances understanding cellular biology, particularly humans model organisms. The application single is fast expanding non‐model taxa, including aquaculture species, where numerous research applications are underway with many more envisaged. In this review, we highlight potential research, considering barriers solutions broad uptake these technologies. Focusing on transcriptomics, outline considerations for experimental design, essential requirement obtain high quality cells/nuclei sequencing ectothermic aquatic species. We further data analysis bioinformatics considerations, tailored studies under‐characterized genomes our knowledge heterogeneity marker genes immature. Overall, review offers useful source researchers aiming apply address challenges faced by global sector though an improved biology.

Language: Английский

Citations

21
Genome Survey of the Freshwater Mussel Venustaconcha ellipsiformis (Bivalvia: Unionida) Using a Hybrid De Novo Assembly Approach DOI Creative Commons
Sébastien Renaut, Davide Guerra,

Walter R. Hoeh

et al.

Genome Biology and Evolution, Journal Year: 2018, Volume and Issue: 10(7), P. 1637 - 1646

Published: June 4, 2018

Freshwater mussels (Bivalvia: Unionida) serve an important role as aquatic ecosystem engineers but are one of the most critically imperilled groups animals. Here, we used a combination sequencing strategies to assemble and annotate draft genome Venustaconcha ellipsiformis, which will valuable genomic resource given ecological value unique "doubly uniparental inheritance" mode mitochondrial DNA transmission freshwater mussels. The described here was obtained by combining high-coverage short reads (65× coverage Illumina paired-end 11× mate-pairs sequences) with low-coverage Pacific Biosciences long (0.3× coverage). Briefly, final scaffold assembly accounted for total size 1.54 Gb (366,926 scaffolds, N50 = 6.5 kb, 2.3% "N" nucleotides), representing 86% predicted 1.80 Gb, while over third (37.5%) consisted repeated elements >85% core eukaryotic genes were recovered. Given genetic bottlenecks V. ellipsiformis populations result glaciations events, heterozygosity also found be remarkably low (0.6%), in contrast other sequenced bivalve species. Finally, reassembled full six polymorphic sites respect previously published reference. This opens way comparative genomics studies identify related adaptations their distinctive inheritance mechanism.

Language: Английский

Citations

45
Deciphering the Link between Doubly Uniparental Inheritance of mtDNA and Sex Determination in Bivalves: Clues from Comparative Transcriptomics DOI Creative Commons

Charlotte Capt,

Sébastien Renaut, Fabrizio Ghiselli

et al.

Genome Biology and Evolution, Journal Year: 2018, Volume and Issue: 10(2), P. 577 - 590

Published: Jan. 18, 2018

Bivalves exhibit an astonishing diversity of sexual systems and sex-determining mechanisms. They can be gonochoric, hermaphroditic or androgenetic, with both genetic environmental factors known to determine influence sex. One unique system involving the mitochondrial genome has also been hypothesized exist in bivalves doubly uniparental inheritance (DUI) mtDNA. However, link between DUI sex determination remains obscure. In this study, we performed a comparative gonad transcriptomics analysis for two DUI-possessing freshwater mussel species better understand mechanisms underlying these bivalves. We used BLAST reciprocal identify orthologs Venustaconcha ellipsiformis Utterbackia peninsularis compared our results previously published sex-specific bivalve transcriptomes conserved genes. data other candidate genes possibly involved regulation DUI. A total ∼12,000 orthologous relationships were found, 2,583 differentially expressed species. Among genes, key reported vertebrates (e.g., Sry, Dmrt1, Foxl2) identified, suggesting that some steps sex-determination pathway may deeply metazoans. Our support hypothesis modified ubiquitination mechanism could responsible retention paternal mtDNA male bivalves, revealed DNA methylation Globally, suggest sets associated are similar distantly-related

Language: Английский

Citations

41
Did doubly uniparental inheritance (DUI) of mtDNA originate as a cytoplasmic male sterility (CMS) system? DOI Creative Commons
Sophie Breton, Donald T. Stewart, Julie Brémaud

et al.

BioEssays, Journal Year: 2022, Volume and Issue: 44(4)

Published: Feb. 16, 2022

Abstract Animal and plant species exhibit an astonishing diversity of sexual systems, including environmental genetic determinants sex, with the latter material in mitochondrial genome. In several hermaphroditic plants for example, sex is determined by interaction between cytoplasmic male sterility (CMS) genes nuclear restorer genes. Specifically, CMS involves aberrant that prevent pollen development specific restore it, leading to a mixture female (male‐sterile) individuals population (gynodioecy). Such mitochondrial‐nuclear determination system thought be rare outside plants. Here, we present one possible case animals. We hypothesize only exception strict maternal mtDNA inheritance animals, doubly uniparental (DUI) bivalves, might have originated as sex‐determination system. document explore similarities exist DUI CMS, propose various ways test our hypothesis.

Language: Английский

Citations

20
Exploring the evolution of sex determination related genes through a cross-species genomics analysis DOI Creative Commons
Filippo Nicolini, Sergey V. Nuzhdin, Fabrizio Ghiselli

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 31, 2025

Abstract The molecular basis of sex determination (SD), while being extensively studied in model organisms, remains poorly understood many animal groups. Bivalves, a diverse class molluscs with variety reproductive modes, represent an ideal yet challenging clade for investigating SD and the evolution sexual systems. However, absence comprehensive framework has limited progress this field, particularly regarding study sex-determination related genes (SRGs). In study, we performed genome-wide sequence evolutionary analysis Dmrt, Sox, Fox gene families more than 40 bivalve species. For first time, provide extensive phylogenetic-aware dataset these SRGs, find support to hypothesis that Dmrt-1L Sox-H may act as primary sex-determining genes, by showing their high levels diversity within genomic context. To validate our findings, same two well-characterized systems, mammals Drosophila . former, found male Sry exhibits pattern amino acid similar bivalves, consistent its role master regulator. contrast, no such was observed among fruit fly cascade, which is controlled chromosomic mechanism. Overall, findings highlight similarities some mammal possibly driven comparable architecture cascades. This work underscores once again importance employing comparative approach when understudied non-model

Language: Английский

Citations

0
A High-Quality Reference Genome for a Parasitic Bivalve with Doubly Uniparental Inheritance (Bivalvia: Unionida) DOI Creative Commons
Chase H. Smith

Genome Biology and Evolution, Journal Year: 2021, Volume and Issue: 13(3)

Published: Feb. 10, 2021

From a genomics perspective, bivalves (Mollusca: Bivalvia) have been poorly explored with the exception for those of high economic value. The bivalve order Unionida, or freshwater mussels, has interest in recent genomic studies due to their unique mitochondrial biology and peculiar life cycle. However, hindered by lack high-quality reference genome. Here, I present genome assembly Potamilus streckersoni using Pacific Bioscience single-molecule real-time long reads 10X Genomics-linked read sequencing. Further, use RNA sequencing from multiple tissue types stages annotate final was far superior any previously published mussel represented 2,368 scaffolds (2,472 contigs) 1,776,755,624 bp, scaffold N50 2,051,244 bp. A proportion comprised repetitive elements (51.03%), aligning characteristics other bivalves. functional annotation returned 52,407 gene models (41,065 protein, 11,342 tRNAs), which concordant estimated number genes species. This genetic resource, along future developing assemblies annotations, will be integral toward unraveling bases ecologically evolutionarily important traits this hyper-diverse group.

Language: Английский

Citations

27
Validating a molecular clock for nudibranchs—No fossils to the rescue DOI Creative Commons
Kara K S Layton, Nerida G. Wilson

Ecology and Evolution, Journal Year: 2024, Volume and Issue: 14(2)

Published: Feb. 1, 2024

Time calibrated phylogenies are typically reconstructed with fossil information but for soft-bodied marine invertebrates that lack hard parts, a record is lacking. In these cases, biogeographic calibrations or the rates of divergence related taxa often used. Although nudibranch have advanced input molecular data, no study has derived rate this diverse group invertebrates. Here, we use an updated closure date Isthmus Panama (2.8 Ma) to derive first chromodorid nudibranchs using multigene data from geminate pair broad phylogeographic sampling. Examining species

Language: Английский

Citations

3
Sex determination in the oyster Crassostrea gigas - A large longitudinal study of population sex ratios and individual sex changes DOI Creative Commons
Coralie Broquard, Anne‐Sophie Martinez,

Élise Maurouard

et al.

Aquaculture, Journal Year: 2019, Volume and Issue: 515, P. 734555 - 734555

Published: Oct. 14, 2019

Language: Английский

Citations

25
Variability of mitochondrial ORFans hints at possible differences in the system of doubly uniparental inheritance of mitochondria among families of freshwater mussels (Bivalvia: Unionida) DOI Creative Commons
Davide Guerra, Manuel Lopes‐Lima, Elsa Froufe

et al.

BMC Evolutionary Biology, Journal Year: 2019, Volume and Issue: 19(1)

Published: Dec. 1, 2019

Supernumerary ORFan genes (i.e., open reading frames without obvious homology to other genes) are present in the mitochondrial genomes of gonochoric freshwater mussels (Bivalvia: Unionida) showing doubly uniparental inheritance (DUI) mitochondria. DUI is a system which distinct female-transmitted and male-transmitted mitotypes coexist single species. In families Unionidae Margaritiferidae, transition from dioecy hermaphroditism loss appear be linked, this event seems affect integrity genes. These observations led hypothesis that ORFans have role and/or sex determination. Complete genome sequences however scarce for most mussels, therefore hindering clear localization various lineages comprehensive understanding influence on sexual systems. Therefore, we sequenced characterized eleven new mitogenomes poorly sampled mussel gather information evolution variability their protein products.We obtained ten complete plus one almost mitogenome sequence representative species (gonochoric hermaphroditic) Hyriidae, Mulleriidae, Iridinidae. only Margaritiferidae while non-DUI Iridinidae, Mulleriidae lack them completely, independently system. Comparisons among proteins translated newly already known ones provide evidence conserved structures, as well family-specific features.The show comparable organization secondary structures different supports physiological role, but also distinctive features. Given latter observation fact can either highly mutated or completely absent secondarily lost depending respective family, hypothesize some aspects connection ORFans, systems, may differ unionids.

Language: Английский

Citations

24
Multi-tissue RNA-Seq Analysis and Long-read-based Genome Assembly Reveal Complex Sex-specific Gene Regulation and Molecular Evolution in the Manila Clam DOI Creative Commons
Ran Xu, Jacopo Martelossi,

Morgan Smits

et al.

Genome Biology and Evolution, Journal Year: 2022, Volume and Issue: 14(12)

Published: Dec. 1, 2022

The molecular factors and gene regulation involved in sex determination gonad differentiation bivalve molluscs are unknown. It has been suggested that doubly uniparental inheritance (DUI) of mitochondria may be these processes species such as the ubiquitous commercially relevant Manila clam, Ruditapes philippinarum. We present first long-read-based de novo genome assembly a RNA-Seq multi-tissue analysis 15 females males. highly contiguous was used reference to investigate expression, alternative splicing, sequence evolution, tissue-specific co-expression networks, sexual contrasting SNPs. Differential expression (DE) differential splicing (DS) analyses revealed sex-specific transcriptional gonads, but not somatic tissues. Co-expression networks complex genes gonad-associated modules showed high tissue specificity. However, male patterns evolution One set related structural organization gametes presented slow pleiotropy, whereas another enriched reproduction-related characterized by fast Sexual SNPs were found overrepresented mitochondrial-related functions, providing new candidates for investigating relationship between DUI species. Together, results increase our understanding role DE, DS, an understudied taxon. also provide resourceful genomic data studies regarding diagnosis breeding bivalves.

Language: Английский

Citations

13