Mesenchymal stem cell exosomes as a cell-free therapy for nerve injury–induced pain in rats DOI

Sheng‐Jie Shiue,

Ruey-Horng Rau,

Han-Shiang Shiue

et al.

Pain, Journal Year: 2018, Volume and Issue: 160(1), P. 210 - 223

Published: Sept. 3, 2018

Abstract Nerve injury–induced neuropathic pain is difficult to treat. In this study, we used exosomes derived from human umbilical cord mesenchymal stem cell (UCMSC) as a cell-free therapy for nerve in rats. Isolated UCMSC range size 30 160 nm and contain CD63, HSP60, CD81 exosome markers. After L5/6 spinal ligation surgery, single intrathecal injection of reversed ligation–induced mechanical thermal hypersensitivities right hindpaw rats at initial well-developed stages. Moreover, continuous infusion achieved excellent preventive reversal effects pain. immunofluorescent lots Exo-green-labelled could be found majorly the ipsilateral L5 dorsal horn, root ganglion, peripheral axons, suggesting homing ability exosomes. They also appeared central terminals or bodies IB4 + , CGRP NF200 sensory neurons. addition, treatment suppressed upregulation c-Fos, CNPase, GFAP, Iba1. All these data suggest that analgesic may involve their actions on neuron glial cells. Exosomes inhibited level TNF-α IL-1β, while enhanced IL-10, brain-derived neurotrophic factor, line–derived factor ganglion nerve-ligated rats, indicating anti-inflammatory proneurotrophic abilities. Protein analysis revealed content vascular endothelial growth C, angiopoietin-2, fibroblast factor-2 summary, UCMSCs considered novel therapeutic approach

Language: Английский

The use of brain organoids to investigate neural development and disease DOI
Elizabeth Di Lullo, Arnold R. Kriegstein

Nature reviews. Neuroscience, Journal Year: 2017, Volume and Issue: 18(10), P. 573 - 584

Published: Sept. 7, 2017

Language: Английский

Citations

672
Biofilm-specific antibiotic tolerance and resistance DOI
Ingar Olsen

European Journal of Clinical Microbiology & Infectious Diseases, Journal Year: 2015, Volume and Issue: 34(5), P. 877 - 886

Published: Jan. 28, 2015

Language: Английский

Citations

655
Adverse Health Consequences of Performance-Enhancing Drugs: An Endocrine Society Scientific Statement DOI Open Access
Harrison G. Pope, Ruth I. Wood, Alan D. Rogol

et al.

Endocrine Reviews, Journal Year: 2013, Volume and Issue: 35(3), P. 341 - 375

Published: Dec. 17, 2013

Despite the high prevalence of performance-enhancing drug (PED) use, media attention has focused almost entirely on PED use by elite athletes to illicitly gain a competitive advantage in sports, and not health risks PEDs. There is widespread misperception that safe or adverse effects are manageable. In reality, vast majority users but rather nonathlete weightlifters, greatly underappreciated. This scientific statement synthesizes available information medical consequences identifies gaps knowledge, aims focus community policymakers as an important public problem. frequently consume highly supraphysiologic doses PEDs, combine them with other PEDs and/or classical drugs abuse, display additional associated risk factors. been linked increased death wide variety cardiovascular, psychiatric, metabolic, endocrine, neurologic, infectious, hepatic, renal, musculoskeletal disorders. Because randomized trials cannot ethically duplicate large many factors we need observational studies collect valid outcome data addition, regarding mechanisms which exert their effects, interactive sports injuries high-risk behaviors. We also assess therapeutic interventions for treating such anabolic-androgen steroid withdrawal syndrome. Finally, raise awareness serious

Language: Английский

Citations

568
An Inflammation-Centric View of Neurological Disease: Beyond the Neuron DOI Creative Commons
Stephen D. Skaper,

Laura Facci,

Morena Zusso

et al.

Frontiers in Cellular Neuroscience, Journal Year: 2018, Volume and Issue: 12

Published: March 21, 2018

Inflammation is a complex biological response fundamental to how the body deals with injury and infection eliminate initial cause of cell effect repair. Unlike normally beneficial acute inflammatory response, chronic inflammation can lead tissue damage ultimately its destruction, often results from an inappropriate immune response. in nervous system ('neuroinflammation'), especially when prolonged, be particularly injurious. While per se may not disease, it contributes importantly disease pathogenesis across both peripheral (neuropathic pain, fibromyalgia) central (e.g. Alzheimer Parkinson multiple sclerosis, motor neuron disease. ischemia traumatic brain injury, depression, autism spectrum disorder) systems. The existence extensive lines communication between represents principle underlying neuroinflammation. Immune cell-derived molecules are critical for regulation host responses inflammation. Although these mediators originate various non-neuronal cells, important sources above neuropathologies appear microglia mast together astrocytes possibly also oligodendrocytes. Understanding neuroinflammation requires appreciation that – interactions, glia cells themselves, integral part process. Within this context occupies key niche orchestrating process, initiation prolongation. This review will describe current state knowledge concerning biology neuroinflammation, emphasizing cell-glia glia-glia then conclude consideration cell’s endogenous mechanisms might leveraged provide therapeutic strategy target

Language: Английский

Citations

393
Opioid-induced hyperalgesia: Cellular and molecular mechanisms DOI

Laurie-Anne Roeckel,

Glenn-Marie Le Coz,

Claire Gavériaux‐Ruff

et al.

Neuroscience, Journal Year: 2016, Volume and Issue: 338, P. 160 - 182

Published: June 24, 2016

Language: Английский

Citations

345
Towards a low-carbon society: A review of lithium resource availability, challenges and innovations in mining, extraction and recycling, and future perspectives DOI
Carlito Baltazar Tabelin, J.A. Dallas, Sophia Casanova

et al.

Minerals Engineering, Journal Year: 2021, Volume and Issue: 163, P. 106743 - 106743

Published: Feb. 10, 2021

Language: Английский

Citations

342
Bacterial Neuroactive Compounds Produced by Psychobiotics DOI
Rebecca Wall, John F. Cryan, R. Paul Ross

et al.

Advances in experimental medicine and biology, Journal Year: 2014, Volume and Issue: unknown, P. 221 - 239

Published: Jan. 1, 2014

Language: Английский

Citations

317
The Crystallization of Monosodium Urate DOI

Miguel Martillo,

Lama Nazzal,

Daria B. Crittenden

et al.

Current Rheumatology Reports, Journal Year: 2013, Volume and Issue: 16(2)

Published: Dec. 20, 2013

Language: Английский

Citations

277
Are intact peptides absorbed from the healthy gut in the adult human? DOI Open Access
Warren Miner-Williams,

Bruce R. Stevens,

Paul J. Moughan

et al.

Nutrition Research Reviews, Journal Year: 2014, Volume and Issue: 27(2), P. 308 - 329

Published: Dec. 1, 2014

For over 100 years it was believed that dietary protein must be completely hydrolysed before its constituent amino acids could absorbed via specific acid transport systems. It is now known the uptake of di- and tripeptides into enterocyte considerable, being transported across intestinal endothelium by PepT1 H + /peptide co-transporter. There also evidence some may survive cytosolic hydrolysis intact basolateral membrane. However, other than antigen sampling, larger macromolecules healthy adult human remains a controversial issue as there little unequivocal in vivo to support this postulation. The aim present review critically evaluate scientific peptides/proteins are epithelia pass hepatic portal system. question absorption oliogopeptides paramount emerging science food-derived bioactive peptides, their mode action physiological effects. Overall, we conclude tripeptides, can cross gut wall enter system physiologically relevant concentrations.

Language: Английский

Citations

225
Evidence of both systemic inflammation and neuroinflammation in fibromyalgia patients, as assessed by a multiplex protein panel applied to the cerebrospinal fluid and to plasma DOI Creative Commons
Emmanuel Bäckryd, Lars Tanum,

Anne‐Li Lind

et al.

Journal of Pain Research, Journal Year: 2017, Volume and Issue: Volume 10, P. 515 - 525

Published: March 1, 2017

In addition to central hyperexcitability and impaired top-down modulation, chronic inflammation probably plays a role in the pathophysiology of fibromyalgia (FM). Indeed, on basis both animal experiments human studies involving analysis cytokines other inflammation-related proteins different body fluids, neuroinflammatory mechanisms are considered be many pain conditions. However, concerning FM, previous plasma/serum and/or cerebrospinal fluid (CSF) cytokine have looked only at few predetermined candidates. Instead analyzing substances time, we used new multiplex protein panel enabling simultaneous 92 proteins. Hence, investigated CSF plasma inflammatory profiles 40 FM patients compared with from healthy controls (n=10) blood donor (n=46). Using multivariate data by projection, found evidence neuroinflammation (as assessed CSF) systemic plasma). Two groups (one for one plasma) highly discriminating between presented. Notably, high levels chemokine CX3CL1 (also known as fractalkine). addition, findings IL-8 were replicated, plasma. This is first time that such an extensive profile has been described patients. seems characterized objective biochemical alterations, lingering characterization its essentially idiopathic or even psychogenic should seen definitively outdated.

Language: Английский

Citations

199